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    MICROORGANISMS AS CARRIERS OF NUCLEOTIDE SEQUENCES CODING FOR ANTIGENS AND PROTEIN TOXINS, PROCESS OF MANUFACTURING AND USES THEREOF
    7.
    发明申请
    MICROORGANISMS AS CARRIERS OF NUCLEOTIDE SEQUENCES CODING FOR ANTIGENS AND PROTEIN TOXINS, PROCESS OF MANUFACTURING AND USES THEREOF 审中-公开
    微生物作为核苷酸序列编码抗原和蛋白毒素的方法，其制造方法及其用途

    公开(公告)号：WO2008058944A1

    公开(公告)日：2008-05-22

    申请号：PCT/EP2007/062237

    申请日：2007-11-13

    申请人： AETERNA ZENTARIS GMBH

    发明人： GENTSCHEV, Ivaylo ,  FENSTERLE, Joachim ,  RAPP, Ulf R. ,  GOEBEL, Werner

    IPC分类号： C12N15/62 ,  A61K39/385

    CPC分类号： C12N15/62 ,  A61K39/00 ,  A61K2039/5156 ,  A61K2039/523 ,  A61K2039/6037 ,  C07K2319/02 ,  C07K2319/035 ,  C07K2319/036 ,  C07K2319/55 ,  C12N15/625 ,  Y02A50/472 ,  Y02A50/476 ,  Y02A50/484

    摘要： The invention relates to a microorganism as a carrier of nucleotide sequences coding for antigens and protein toxins comprising the following components: (I) at least one nucleotide sequence coding for at least one complete or partial antigen of at least one wild-type or mutated protein; and (II) at least one nucleotide sequence coding for at least one protein toxin and/or at least one protein toxin subunit; and (III) a) at least one nucleotide sequence coding for at least one transport system which enables the expression of the expression products of component (I) and component (II) on the outer surface of the microorganism and/or enables the secretion of the expression products of component (I) and component (II); and/or coding for at least one signal sequence which enables the secretion of the expression products of component (I) and component (II); and/or (III) b) optionally, at least one nucleotide sequence coding for at least one protein for lysing the microorganism in the cytosol of mammalian cells and for intracellularly releasing plasmids or expression vectors, which are contained in the lysed microorganism; and (IV) at least one nucleotide sequence for at least one activation sequence for the expression of one or more of components (I) to (III), wherein said activation sequence can be activated in the microorganism and/or is tissue cell-specific, tumor cell-specific, macrophage-specific, dendrite-specific, lymphocyte-specific, function-specific or non-cell-specific"; wherein any of components (I) to (IV) can be present either once or several times and either identical or different. Also disclosed are a process of manufacturing thereof, corresponding plasmids or expression vectors and uses of the microorganism as a medicament.

    摘要翻译： 本发明涉及作为编码抗原和蛋白质毒素的核苷酸序列的载体的微生物，其包含以下组分：（I）编码至少一种野生型或突变型蛋白质的至少一种完整或部分抗原的至少一种核苷酸序列 ; 和（II）编码至少一种蛋白质毒素和/或至少一种蛋白质毒素亚基的至少一个核苷酸序列; 和（III）a）至少一个编码至少一种转运系统的核苷酸序列，其能够将组分（I）和组分（II）的表达产物表达在微生物的外表面上和/或使得能够分泌 组分（I）和组分（II）的表达产物; 和/或编码能够分泌组分（I）和组分（II）的表达产物的至少一种信号序列; 和/或（III）b）任选地至少一种编码至少一种蛋白质的核酸序列，用于裂解哺乳动物细胞的细胞质中的微生物和细胞内释放质粒或表达载体，所述质粒或表达载体包含在裂解的微生物中; 至少一个用于表达一种或多种组分（I）至（III）的活化序列的至少一个核苷酸序列，其中所述活化序列可以在微生物中活化和/或是组织细胞特异性 ，肿瘤细胞特异性，巨噬细胞特异性，枝晶特异性，淋巴细胞特异性，功能特异性或非细胞特异性“;其中组分（I）至（IV）中的任何一种可以存在一次或数次， 还公开了其制备方法，相应的质粒或表达载体以及微生物作为药物的用途。