公开(公告)号：WO2007112794A1

公开(公告)日：2007-10-11

申请号：PCT/EP2006/070265

申请日：2006-12-29

Applicant: KERYOS SPA ,  VERONESE, Francesco Maria ,  PASUT, Gianfranco ,  TONON, Giancarlo ,  SCHREPFER, RODOLFO

Inventor： VERONESE, Francesco Maria ,  PASUT, Gianfranco ,  TONON, Giancarlo ,  SCHREPFER, RODOLFO

IPC: C08G65/329 ,  A61K47/48

CPC classification number: C08G65/329 ,  A61K47/60 ,  C07K1/1077 ,  C08G65/333

Abstract: There are disclosed chemically active poly(ethylene glycols) and other hydrophilic polymers that are suitable for coupling to pharmaceutically or diagnostically active agents such as peptides, oligonucleotides, proteins or non- peptide molecules. The compounds are represented by the formula Poly-(X-NH-CO-A) n wherein: Poly is a hydrophilic polymer having a molecular weight of from about 300 to 100000 Daltons; A together with -NH-CO- forms a reactive group; X is a spacer moiety or a bond; n is an integer comprised between 1 and 50. The active agents of interest which may be conjugated to the disclosed compounds may be selected from hemoglobin, insulin, urokinase, alpha- interferon, G-CSF, hGH, asparaginase, adenosine deaminase, superoxide dismutase and catalase.

Abstract translation: 公开了化学活性聚（乙二醇）和其它亲水性聚合物，其适于与药学或诊断活性剂如肽，寡核苷酸，蛋白质或非肽分子偶联。 所述化合物由下式表示：Poly-（X-NH-CO-A）n N n其中：Poly是分子量为约300至100000道尔顿的亲水性聚合物; A与-NH-CO-一起形成反应性基团; X是间隔部分或键; 可以与所公开的化合物缀合的感兴趣的活性剂可以选自血红蛋白，胰岛素，尿激酶，α-干扰素，G-CSF，hGH，天冬酰胺酶，腺苷脱氨酶，超氧化物歧化酶 和过氧化氢酶。

公开(公告)号：WO0240652A3

公开(公告)日：2002-10-31

申请号：PCT/EP0113257

申请日：2001-11-14

Applicant: KERYOS SPA ,  VANONI MARCO ,  TORTORA PAOLO ,  TONON GIANCARLO ,  TAYLOR JEOFFREY ,  ORSINI GAETANO

Inventor： VANONI MARCO ,  TORTORA PAOLO ,  TONON GIANCARLO ,  TAYLOR JEOFFREY ,  ORSINI GAETANO

IPC: C12N15/09 ,  C12N1/19 ,  C12N9/60 ,  C12N15/57 ,  C12P21/06 ,  C12N5/10 ,  C12P21/00

CPC classification number: C12N9/60 ,  C12P21/06

Abstract: The present invention relates to the expression and secretion in Saccharomyces cerevisiae of readily purifiable soluble variants of the Kex1 endopeptidase of Kluyveromyces lactis in vitro processing of recombinant proteins usable in industrial applications. The soluble Kex1 endoproteases described here are free from the transmembrane domain of the native enzyme; the deletion of the transmembrane domain is achieved by removing at least 57 amino acid residues from the C-terminal.

Abstract translation: 本发明涉及酿酒酵母Kex1内肽酶的易于纯化的可溶性变体的酿酒酵母中的表达和分泌，并且其纯化和用于本发明的第i＆ 可用于工业应用的重组蛋白的体外加工。 这里描述的可溶性Kex1内切蛋白酶不含天然酶的跨膜结构域; 通过从C-末端去除至少57个氨基酸残基来实现跨膜结构域的缺失。

公开(公告)号：WO2003031630A1

公开(公告)日：2003-04-17

申请号：PCT/IT2002/000646

申请日：2002-10-10

Applicant: KERYOS SPA ,  FAZIO, VITO ,  RINALDI, Monica ,  SONZOGNI, Laura ,  TONON, Giancarlo ,  ORSINI, Gaetano

Inventor： FAZIO, VITO ,  RINALDI, Monica ,  SONZOGNI, Laura ,  TONON, Giancarlo ,  ORSINI, Gaetano

IPC: C12N15/63

CPC classification number: C12N15/85 ,  A61K48/00 ,  A61K2039/53 ,  C12N2830/42 ,  C12N2840/203 ,  C12N2840/206

Abstract: The subject of the present invention is the construction of multicistronic eukaryotic plasmid expression vectors in which it is possible to express from two to four genes simultaneously and which are characterized by differently regulated bicistronic transcription units. The distinctive characteristic of these vectors is the presence of a CAP-independent translation initiation mechanism which is based on the ability of an IRES (internal ribosomal entry site) sequence to translate two proteins under the control of a single promoter. This family of multicistronic vectors can advantageously be used in various biotechnological applications in whcih the simultaneous expression of two or more genes is necessary, such as gene transfer protocols, DNA-immunization, or for the expression of different molecules in the same cell.

Abstract translation: 本发明的主题是多顺反子真核质粒表达载体的构建，其中可以同时从两个到四个基因表达，其特征在于不同程度的双顺反子转录单位。 这些载体的独特特征是存在CAP独立的翻译起始机制，其基于IRES（内部核糖体进入位点）序列在单个启动子的控制下翻译两种蛋白质的能力。 这种多顺反子载体家族可有利地用于各种生物技术应用中，同时表达两个或多个基因是必需的，例如基因转移方案，DNA免疫或用于在相同细胞中表达不同分子。