公开(公告)号：WO2010147920A1

公开(公告)日：2010-12-23

申请号：PCT/US2010/038572

申请日：2010-06-15

Applicant: MBI INTERNATIONAL ,  GUETTLER, Michael, V. ,  HANCHAR, Robert, J. ,  RUMLER, Denise, S. ,  KLEFF, Susanne

Inventor： GUETTLER, Michael, V. ,  HANCHAR, Robert, J. ,  RUMLER, Denise, S. ,  KLEFF, Susanne

IPC: C12P7/46

CPC classification number: C12P7/56 ,  C12P7/46 ,  C12P7/48

Abstract: This invention is directed to methods of obtaining a high quantity of desired acid salt in a form that is relatively easy to recover. Particularly desired as a recovery product is an organic acid. A salt form of the desired organic acid is formed that is relatively high in concentration and that is relatively soluble in an aqueous medium. The method includes a step of fermenting a fermentable carbon source in the fermentation medium in the presence of a magnesium compound containing oxygen.

Abstract translation: 本发明涉及以相当容易恢复的形式获得大量所需酸盐的方法。 作为回收产物特别需要的是有机酸。 形成浓度相对较高并且相对可溶于水性介质的所需有机酸的盐形式。 该方法包括在含有氧化镁的化合物的存在下在发酵培养基中发酵可发酵碳源的步骤。

公开(公告)号：WO2006083410A3

公开(公告)日：2006-11-23

申请号：PCT/US2005045714

申请日：2005-12-16

Applicant: MICHIGAN BIOTECH INST ,  YI JIAN ,  KLEFF SUSANNE ,  GUETTLER MICHAEL V

Inventor： YI JIAN ,  KLEFF SUSANNE ,  GUETTLER MICHAEL V

IPC: C12N1/20 ,  C12N15/74

CPC classification number: C12N9/0006 ,  C12P7/46

Abstract: Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf ) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

Abstract translation: 公开了用于生产有机酸的重组微生物。 重组微生物表达具有用于磷酸戊糖循环中的酶的酶活性的多肽。 重组微生物可以包括已经转化以表达Zwischenferment（Zwf）基因的重组放线杆菌（Actinobacillus succinogenes）。 重组微生物可用于生产有机酸如琥珀酸和乳酸的发酵过程。 还公开了可用于转化微生物以产生表达诸如Zwf的酶的重组微生物的新型质粒。

公开(公告)号：WO2012119064A1

公开(公告)日：2012-09-07

申请号：PCT/US2012/027450

申请日：2012-03-02

Applicant: MICHIGAN BIOTECHNOLOGY INSTITUTE ,  HANCHAR, Robert, J. ,  KLEFF, Susanne ,  GUETTLER, Michael, V.

Inventor： HANCHAR, Robert, J. ,  KLEFF, Susanne ,  GUETTLER, Michael, V.

IPC: C07C51/02 ,  C01C1/24 ,  C01D1/20 ,  C07C55/02 ,  C07C55/06 ,  C07C55/10 ,  C07C55/22 ,  C07C57/13 ,  C07C57/15 ,  C12P7/46 ,  C12P7/48

CPC classification number: C01C1/24 ,  C01D1/04 ,  C01F5/14 ,  C01F11/02 ,  C07C51/02 ,  C12P7/46 ,  C12P7/48 ,  C07C55/02 ,  C07C55/06 ,  C07C55/10 ,  C07C55/22 ,  C07C57/13 ,  C07C57/15

Abstract: This invention provide processes for producing carboxylic acid product, along with useful salts. The carboxylic acid product that is produced according to this invention is preferably a C 2 -C 12 carboxylic acid. Among the salts produced in the process of the invention are ammonium salts.

Abstract translation: 本发明提供了生产羧酸产物以及有用的盐的方法。 根据本发明制备的羧酸产物优选为C 2 -C 12羧酸。 在本发明方法中生产的盐是铵盐。

公开(公告)号：WO2006083410A2

公开(公告)日：2006-08-10

申请号：PCT/US2005/045714

申请日：2005-12-16

Applicant: MICHIGAN BIOTECHNOLOGY INSTITUTE ,  YI, Jian ,  KLEFF, Susanne ,  GUETTLER, Michael, V.

Inventor： YI, Jian ,  KLEFF, Susanne ,  GUETTLER, Michael, V.

IPC: C12N1/21

CPC classification number: C12N9/0006 ,  C12P7/46

Abstract: Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf ) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

Abstract translation: 公开了用于生产有机酸的重组微生物。 重组微生物表达具有在戊糖磷酸循环中使用的酶的酶活性的多肽。 重组微生物可以包含已经被转化以表达Zwischenferment（Zwf）基因的重组放线杆菌属的基因。 重组微生物可用于生产有机酸如琥珀酸和乳酸的发酵方法。 还公开了可用于转化微生物以产生表达诸如Zwf之类的酶的重组微生物的新质粒。