公开(公告)号：WO2019213033A1

公开(公告)日：2019-11-07

申请号：PCT/US2019/029827

申请日：2019-04-30

Applicant: INVISTA NORTH AMERICA S.A.R.L. ,  INVISTA TEXTILES (U.K.) LIMITED

Inventor： FOSTER, Alexander, Brett ,  FRITSCH, Emilie, Sophie

IPC: C12N15/52 ,  C12N9/88 ,  C12N9/00 ,  C12N9/10 ,  C12P7/56 ,  C12P7/06 ,  C12P7/28 ,  C12P7/54 ,  C12P7/46 ,  C12P7/42 ,  C12P7/18 ,  C12P7/48 ,  C12P7/44 ,  C12P13/14 ,  C12P13/00 ,  C12P13/08 ,  C12P1/04

Abstract: Methods of redirecting carbon flux and increasing C2/C3 or a C4/5/6 carbon chain length carbon-based chemical product yield in an organism, nonnaturally occurring organisms with redirected carbon flux and increased C2/C3 or C4/5/6 carbon chain length carbon-based chemical product yield and methods for using these organisms in production of C2/C3 or C4/5/6 carbon chain length carbon-based chemical products are provided.

公开(公告)号：WO2014136028A1

公开(公告)日：2014-09-12

申请号：PCT/IB2014/059343

申请日：2014-02-28

Applicant: INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

Inventor： NICAUD, Jean-Marc ,  BEOPOULOS, Athanasios ,  PAPANIKOLAOU, Séraphim

IPC: C12N9/88 ,  C12P7/48 ,  C12P7/64

CPC classification number: C12N9/88 ,  C12P7/48 ,  C12P7/6463 ,  C12Y402/01079

Abstract: La présente invention concerne une souche mutante de levure dans laquelle au moins l'expression ou l'activité de la 2-méthylcitrate déshydratase est inhibée, et l'utilisation de cette souche pour la production de lipides et d'acide citrique.

Abstract translation: 本发明涉及至少抑制2-甲基柠檬酸脱水酶的表达或活性的突变型酵母菌株，以及所述菌株用于制备脂质和柠檬酸的突变体酵母菌株。

公开(公告)号：WO2009006909A1

公开(公告)日：2009-01-15

申请号：PCT/EE2008/000018

申请日：2008-07-09

Applicant: NORDBIOCHEM OÜ ,  ILUSHKA, Igor Valerianovich ,  RAKITIN, Vladimir Yuriyvich ,  SHVETS, Valeriy Fedorovich ,  KOZLOVSKIY, Roman Anatolievich ,  SUCHKOV, Yuri Pavlovich ,  KOLBAROV, Vambola Vijacheslavovich

Inventor： ILUSHKA, Igor Valerianovich ,  RAKITIN, Vladimir Yuriyvich ,  SHVETS, Valeriy Fedorovich ,  KOZLOVSKIY, Roman Anatolievich ,  SUCHKOV, Yuri Pavlovich ,  KOLBAROV, Vambola Vijacheslavovich

IPC: C12P7/48 ,  C12P7/54 ,  C12P7/56

CPC classification number: C12P7/48 ,  C12M29/04 ,  C12M29/18 ,  C12M41/22 ,  C12M41/26 ,  C12M47/10 ,  C12P7/54 ,  C12P7/56 ,  Y02P20/544

Abstract: According to present invention, the lactic acid and other organic acids can be produced in a simple and inexpensive manner, so as to eliminate the disadvantages of the prior processes discussed above. It has been surprisingly found, that if to create such conditions at which growth rate of cells is minimal but sufficient for replacement cells death, in this case decrease of the specific consumption of carbohydrates per unit of lactate is take place. The growth rate of cells can be reduced by increase of temperature and decrease of pH up to critical and supercritical values for each microorganism and as well by decrease of amounts nutrients fed into the fermentor and by use of additives bacteriostatics. Preferably, the optimal growth rate of cells reach by combination of temperature, pH, by amount of used nutrients and bacteriostatics fed into the fermentor.

Abstract translation: 根据本发明，乳酸等有机酸可以以简单廉价的方式制造，以消除上述现有方法的缺点。 已经令人惊讶地发现，如果要产生细胞生长速率最小但足以替代细胞死亡的这种条件，在这种情况下，发生每单位乳酸盐的碳水化合物的特定消耗量的降低。 通过增加温度和降低每个微生物的关键和超临界值以及通过减少喂入发酵罐的营养物质和通过使用添加剂抑菌剂，可以降低细胞的生长速率。 优选地，通过温度，pH，通过进料到发酵罐中使用的营养物和抑菌剂的量的组合来达到细胞的最佳生长速率。

公开(公告)号：WO2007063133A3

公开(公告)日：2007-09-13

申请号：PCT/EP2006069218

申请日：2006-12-01

Applicant: DSM IP ASSETS BV ,  BAUWELEERS HUGO MARC KAREL ,  GROESENEKEN DOMINIQUE ROBERT ,  PEIJ VAN NOEL NICOLAAS MARIA E

Inventor： BAUWELEERS HUGO MARC KAREL ,  GROESENEKEN DOMINIQUE ROBERT ,  PEIJ VAN NOEL NICOLAAS MARIA E

IPC: C07K14/38 ,  C12P7/48

CPC classification number: C12P7/48 ,  C07K14/38

Abstract: The present invention relates to newly identified genes that encode proteins that are involved in the (bio)synthesis of citric acid. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of citric acid from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for production of citric acid.

Abstract translation: 本发明涉及编码参与柠檬酸（生物）合成的蛋白质的新鉴定的基因。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 本发明还涉及所述多核苷酸和多肽作为生物技术工具在从微生物生产柠檬酸中的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其生产柠檬酸的用途。

公开(公告)号：WO9205270A3

公开(公告)日：1992-08-20

申请号：PCT/US9106897

申请日：1991-09-23

Applicant: UNIV TEXAS

Inventor： GOUX WARREN J

IPC: C12P1/00 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/04 ,  C12P13/06 ,  C12P13/20 ,  C12P19/02 ,  C12P19/30

CPC classification number: C12P19/30 ,  C12P1/00 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/04 ,  C12P13/06 ,  C12P13/20 ,  C12P19/02

Abstract: The present invention relates to a general method of enzymatic synthesis of isotopically labeled carbohydrates, sugars and nucleosides. Labeled citric acid cycle intermediates, amino acids and ribose mononucleotides may be rapidly and conveniently synthesized from labeled pyruvate, lactate or L-alanine. The method employs a novel nicotinamide dinucleotide regeneration system which permits use of low NADH levels. The method may be manipulated to allow labeling at a variety of carbon/hydrogen sites.

公开(公告)号：WO2020042481A1

公开(公告)日：2020-03-05

申请号：PCT/CN2018/123053

申请日：2018-12-24

Applicant: 江苏国信协联能源有限公司 ,  江南大学

Inventor： 石贵阳 ,  胡志杰 ,  李由然 ,  蒋小东 ,  金赛 ,  孙福新 ,  张成 ,  周东姣 ,  卢佳伟 ,  苗茂栋 ,  范子豪

IPC: C12P7/48 ,  C12N1/14 ,  C12R1/685

Abstract: 一种黑曲霉种子连续培养及其生产柠檬酸的方法，黑曲霉种子连续培养方法包括：（1）启动阶段，将黑曲霉孢子接种至种子培养基，得到种子液；（2）种子连续培养阶段，对步骤（1）得到的种子液进行连续分散处理，分散得到的种子液进行连续培养，并同时补充新鲜种子补料培养基；（3）停止阶段，停止补充新鲜种子补料培养基和分散，继续培养得到种子液，移入发酵培养基进行发酵培养。

公开(公告)号：WO2018233851A1

公开(公告)日：2018-12-27

申请号：PCT/EP2017/065589

申请日：2017-06-23

Applicant: HELMHOLTZ-ZENTRUM FÜR UMWELTFORSCHUNG GMBH - UFZ

Inventor： AURICH, Andreas ,  HUNGER, Steffi ,  LEE, Mi-Yong ,  KOHLHEB, Norbert ,  MÜLLER, Roland Arno

IPC: C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/48 ,  C12N9/88

Abstract: Um ein Verfahren zur biotechnologischen Herstellung von Carbonsäuren, bei der die säurebildenden Mikroorganismen unsterilin einer Abwässer enthaltenden submersen Phase mit und aller für die Herstellung der Carbonsäuren erforderlichen Kohlenstoff-und Nährmedienkomponenten kultviert werden, bereitzustellen, das die Nachteile bekannter Verfahren vermeidet und das eine hohe Produktkonzentrationen und Produktivitäten bei gleichzeitiger Schonung der Ressourcen Wasser und Energie ermöglicht, wird vorgeschlagen, dass Mikroorganismen, die unter unsterilen Bedingungen in einem Kultivierungsmedium, welches Abwasser enthält, unter Zugabe von kohlenstoffreichen Verbindungen kultiviert werden, eingesetzt werden.

公开(公告)号：WO2015061289A1

公开(公告)日：2015-04-30

申请号：PCT/US2014/061529

申请日：2014-10-21

Applicant: DANISCO US INC

Inventor： DUAN, Gang ,  SHETTY, Jayarama K. ,  WANG, Xin ,  XU, Hongxian ,  ZHANG, Xiaoping ,  ZHOU, Peng

IPC: C12P19/14 ,  C12Q1/48 ,  C12P7/48 ,  C12P7/56 ,  C12P13/08 ,  C12P13/14 ,  G01N33/48

CPC classification number: C12P13/14 ,  C12P7/48 ,  C12P7/56 ,  C12P13/08 ,  C12P19/14

Abstract: The present invention relates to methods for improving fermentation processes, including increasing product yield, reduced viscosity, and/or reduced foaming.

Abstract translation: 本发明涉及改善发酵过程的方法，包括提高产物收率，降低粘度和/或减少发泡。

公开(公告)号：WO2012160255A1

公开(公告)日：2012-11-29

申请号：PCT/FI2012/050481

申请日：2012-05-22

Applicant: UPM-KYMMENE CORPORATION ,  RISTOLAINEN, Matti ,  NUORTILA-JOKINEN, Jutta ,  HASSINEN, Esa ,  ESKELINEN, Kati

Inventor： RISTOLAINEN, Matti ,  NUORTILA-JOKINEN, Jutta ,  HASSINEN, Esa ,  ESKELINEN, Kati

IPC: C12P7/42 ,  C12P7/48 ,  C12P7/56 ,  C12M1/36 ,  D21C9/10

CPC classification number: C12P7/42 ,  C12P7/56 ,  C12P2203/00 ,  D21C3/02 ,  D21C3/04 ,  D21C11/0042 ,  D21C11/005

Abstract: The invention relates to a method and an apparatus for manufacturing a hydroxycarboxylic acid product from lignocellulose-based raw material. According to the invention,a first effluent (2) used as a raw material is concentrated to provide a concentrate (3), the concentrate (3) is treated by a hydrolysis (11) to form saccharides (4), the saccharides (4) are fermented (12) to form hydroxycarboxylic acid, and the pH of the fermentation (12) is controlled by using a second effluent (16), for forming a hydroxycarboxylic acid product. Further, the invention relates to use of a bleaching filtrate.

Abstract translation: 本发明涉及一种由木质纤维素基原料制造羟基羧酸产品的方法和装置。 根据本发明，将用作原料的第一流出物（2）浓缩以提供浓缩物（3），通过水解（11）处理浓缩物（3）以形成糖（4），糖（4） ）发酵（12）以形成羟基羧酸，并且通过使用第二流出物（16）来控制发酵（12）的pH，以形成羟基羧酸产物。 此外，本发明涉及漂白滤液的用途。

公开(公告)号：WO2012119064A1

公开(公告)日：2012-09-07

申请号：PCT/US2012/027450

申请日：2012-03-02

Applicant: MICHIGAN BIOTECHNOLOGY INSTITUTE ,  HANCHAR, Robert, J. ,  KLEFF, Susanne ,  GUETTLER, Michael, V.

Inventor： HANCHAR, Robert, J. ,  KLEFF, Susanne ,  GUETTLER, Michael, V.

IPC: C07C51/02 ,  C01C1/24 ,  C01D1/20 ,  C07C55/02 ,  C07C55/06 ,  C07C55/10 ,  C07C55/22 ,  C07C57/13 ,  C07C57/15 ,  C12P7/46 ,  C12P7/48

CPC classification number: C01C1/24 ,  C01D1/04 ,  C01F5/14 ,  C01F11/02 ,  C07C51/02 ,  C12P7/46 ,  C12P7/48 ,  C07C55/02 ,  C07C55/06 ,  C07C55/10 ,  C07C55/22 ,  C07C57/13 ,  C07C57/15

Abstract: This invention provide processes for producing carboxylic acid product, along with useful salts. The carboxylic acid product that is produced according to this invention is preferably a C 2 -C 12 carboxylic acid. Among the salts produced in the process of the invention are ammonium salts.

Abstract translation: 本发明提供了生产羧酸产物以及有用的盐的方法。 根据本发明制备的羧酸产物优选为C 2 -C 12羧酸。 在本发明方法中生产的盐是铵盐。