公开(公告)号：WO2012067572A1

公开(公告)日：2012-05-24

申请号：PCT/SE2011/051369

申请日：2011-11-15

申请人： SCANDINAVIAN TECHNOLOGY GROUP AB ,  ALBERS, Eva ,  OLSSON, Lisbeth ,  KOPPRAM, Rakesh

发明人： ALBERS, Eva ,  OLSSON, Lisbeth ,  KOPPRAM, Rakesh

IPC分类号： C12N15/01 ,  C12N1/36 ,  C12N1/19 ,  C12P7/10 ,  C12R1/865

CPC分类号： C12P7/06 ,  C12N9/0006 ,  C12N9/1205 ,  C12N15/01 ,  C12N15/81 ,  C12P7/10 ,  C12Q1/04 ,  C12R1/865 ,  C12Y101/01009 ,  C12Y101/0101 ,  C12Y101/01307 ,  C12Y207/01017 ,  Y02E50/16 ,  Y02E50/17

摘要： A method for producing astrain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion and reduced xylitol production is described. The method comprises culturing the cells in a repetitive batch series in a medium at a xylose concentration of about 15-25 g/l and at a temperature of about 28- 32°C, and thereafter lowering the xylose concentration in at least one step at a temperature of about 28-32°C to obtain an increased selection pressure for improved xylose fermentation,improved ethanol production and reduced xylitol production, and continuing the culturing of the cells in said repetitive batch series. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.

摘要翻译： 描述了用引入的木糖还原酶，木糖醇脱氢酶和木酮糖激酶基因产生酿酒酵母的方法，并且改进了乙醇生产，改善了木糖转化和降低的木糖醇生产。 该方法包括以约15-25g / l的木糖浓度和约28-32℃的温度在培养基中以重复批次序列培养细胞，然后在至少一步中降低木糖浓度 温度为约28-32℃，以获得用于改进的木糖发酵，改善的乙醇生产和减少的木糖醇生产的增加的选择压力，并且在所述重复批次系列中继续培养细胞。 此外，描述通过根据本发明的方法获得的酿酒酵母菌株。

公开(公告)号：WO2012067571A1

公开(公告)日：2012-05-24

申请号：PCT/SE2011/051368

申请日：2011-11-15

申请人： Scandinavian Technology Group AB ,  ALBERS, Eva ,  OLSSON, Lisbeth ,  KOPPRAM, Rakesh

发明人： ALBERS, Eva ,  OLSSON, Lisbeth ,  KOPPRAM, Rakesh

IPC分类号： C12N15/01 ,  C12N1/36 ,  C12N1/19 ,  C12P7/10 ,  C12R1/865

CPC分类号： C12P7/06 ,  C12N9/0006 ,  C12N9/1205 ,  C12N15/01 ,  C12N15/81 ,  C12P7/10 ,  C12Q1/04 ,  C12R1/865 ,  C12Y101/01009 ,  C12Y101/0101 ,  C12Y101/01307 ,  C12Y207/01017 ,  Y02E50/16 ,  Y02E50/17

摘要： A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25- 38 °C, preferably 30-35 °C, and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.

摘要翻译： 描述了引入了编码木糖还原酶，木糖醇脱氢酶和木酮糖激酶的基因并且改进的乙醇生产，改善的木糖转化，降低的木糖醇产生和改进的抑制剂耐受性的酿酒酵母菌株的方法。 该方法包括以连续模式培养酿酒酵母菌株，其中培养基基本上只含有木糖作为碳源，温度为25-38℃，优选30-35℃，气流为0.040-0.055vvm，和 增加稀释率以保持恒定的细胞水平，所述细胞水平在通过光密度或等效分析方法测定的1.5-3.0的范围内，并且向细胞中加入至少一种抑制剂并逐渐增加所述抑制剂的添加。 此外，描述了通过根据本发明的方法获得的酿酒酵母菌株。