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    COMBINATORIAL DESIGN OF HIGHLY EFFICIENT HETEROLOGOUS PATHWAYS
    2.
    发明申请
    COMBINATORIAL DESIGN OF HIGHLY EFFICIENT HETEROLOGOUS PATHWAYS 审中-公开
    高效率异常路径的组合设计

    公开(公告)号：WO2012045088A9

    公开(公告)日：2012-07-26

    申请号：PCT/US2011054650

    申请日：2011-10-03

    申请人： UNIV ILLINOIS ,  ZHAO HUIMIN ,  KIM BYOUNGJIN ,  DU JING ,  YUAN YONGBO ,  ERIKSEN DAWN ,  SI TONG

    发明人： ZHAO HUIMIN ,  KIM BYOUNGJIN ,  DU JING ,  YUAN YONGBO ,  ERIKSEN DAWN ,  SI TONG

    IPC分类号： C12N15/52

    CPC分类号： C12P7/06 ,  C07K14/39 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/2445 ,  C12N15/1093 ,  C12N15/52 ,  C12N15/81 ,  C12P7/10 ,  C12Y101/01009 ,  C12Y101/0101 ,  C12Y101/01307 ,  C12Y207/01017 ,  C12Y302/01021 ,  Y02E50/16 ,  Y02E50/17

    摘要： The present disclosure relates to the production of highly efficient heterologous pathways in host cells by identifying favorable enzyme and/or promoter combinations. In particular the present disclosure provides methods for assembly and selection of multi-step xylose and arabinose/xylose utilization pathways from a library of fungal enzymes. The present disclosure further provides compositions containing favorable enzyme combinations, as well as recombinant yeast expressing such combinations, and methods of use for bioconversion of pentose sugars. Also provided are compositions and methods involving favorable expression patterns identified by utilization of combinations of promoters of varying strengths. Provided herein are methods for assembly and selection of multi-step xylose, arabinose/xylose, and cellobiose utilization pathways from a library of promoters of varying strengths. The present disclosure further provides compositions containing heterologous enzyme-coding polynucleotides under the control of favorable promoters, as well as recombinant yeast expressing such enzymes, and methods of their use for bioconversion of pentose and/or hexose sugars.

    摘要翻译： 本公开涉及通过鉴定有利的酶和/或启动子组合在宿主细胞中产生高效的异源途径。 特别地，本公开提供了从真菌酶文库中组装和选择多步木糖和阿拉伯糖/木糖利用途径的方法。 本公开进一步提供含有有利的酶组合的组合物，以及表达这种组合的重组酵母以及用于戊糖的生物转化的方法。 还提供了包括通过利用不同强度的启动子的组合鉴定的有利的表达模式的组合物和方法。 本文提供了用于从不同强度的启动子文库中组装和选择多步木糖，阿拉伯糖/木糖和纤维二糖利用途径的方法。 本公开进一步提供了含有有利启动子控制的异源酶编码多核苷酸的组合物，以及表达这些酶的重组酵母，以及它们用于戊糖和/或己糖的生物转化的方法。

    VARIANTS OF GAL2 TRANSPORTER AND THEIR USES
    6.
    发明申请
    VARIANTS OF GAL2 TRANSPORTER AND THEIR USES 审中-公开
    GAL2运输车的变化及其用途

    公开(公告)号：WO2016062823A1

    公开(公告)日：2016-04-28

    申请号：PCT/EP2015/074522

    申请日：2015-10-22

    申请人： BUTALCO GMBH

    发明人： BOLES, Eckhard ,  DIETZ, Heiko ,  FARWICK, Alexander ,  SCHADEWEG, Virginia ,  OREB, Mislav

    IPC分类号： C07K14/395 ,  C12N15/81 ,  C12P7/08 ,  C12P7/12

    CPC分类号： C07K14/395 ,  C12N1/18 ,  C12N9/1205 ,  C12N9/90 ,  C12N9/92 ,  C12N15/52 ,  C12N15/81 ,  C12N15/905 ,  C12P7/06 ,  C12P7/10 ,  C12P7/12 ,  C12Y207/01016 ,  C12Y207/01017 ,  C12Y501/03004 ,  C12Y503/01003 ,  C12Y503/01005 ,  Y02E50/16

    摘要： The present invention relates to polypeptides which are Gal2 variants comprising at least one amino acid substitution at a position corresponding to T354, and optionally further amino acid substitution(s). The present invention further relates to nucleic acid molecules encoding the polypeptides and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of bioethanol and/or other bio-based compounds, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the polypeptides, nucleic acids molecule or host cells for the production of bioethanol and/or other bio-based compounds, and/or for the recombinant fermentation of biomaterial containing pentose(s), preferably D-xylose and/or L-arabinose.

    摘要翻译： 本发明涉及多肽，其是在对应于T354的位置包含至少一个氨基酸取代的Gal2变体，以及任选地进一步的氨基酸取代。 本发明还涉及编码多肽的核酸分子和含有所述核酸分子的宿主细胞。 本发明还涉及用于生产生物乙醇和/或其它生物基化合物的方法，其包括优选在所述宿主细胞中表达所述核酸分子。 本发明还涉及多肽，核酸分子或宿主细胞用于生产生物乙醇和/或其他基于生物的化合物和/或用于重组发酵含有戊糖的生物材料，优选D- 木糖和/或L-阿拉伯糖。

    GLUCOSE AND XYLOSE CO-FERMENTING MICROORGANISM THAT EXPRESSES ACTIVE GLUCOAMYLASE
    7.
    发明申请
    GLUCOSE AND XYLOSE CO-FERMENTING MICROORGANISM THAT EXPRESSES ACTIVE GLUCOAMYLASE 审中-公开
    GLUCOSE和XYLOSE表达活性GLUCOAMYLASE的共同发酵微生物

    公开(公告)号：WO2016036621A1

    公开(公告)日：2016-03-10

    申请号：PCT/US2015/047625

    申请日：2015-08-30

    申请人： TEKKWARE, INC.

    发明人： HO, Nancy, W., Y.

    IPC分类号： C12M1/00 ,  C12N1/19 ,  C12N15/00 ,  C12P7/06

    CPC分类号： C12P7/10 ,  C10L1/02 ,  C10L2200/0469 ,  C10L2290/26 ,  C12N1/18 ,  C12N1/22 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/2428 ,  C12Y101/01 ,  C12Y207/01017 ,  C12Y302/01003 ,  Y02E50/16 ,  Y02E50/17

    摘要： Provided are microorganisms, e.g., the Saccharomyces yeast, that have been made able to co-ferment xylose sugar obtained from hydrolyzing plant cellulosic biomass from trees, grasses, straws, etc., with glucose that can be obtained from hydrolyzing either edible feedstocks such as starch, cane sugar, etc., or from hydrolyzing cellulose from various types of non-edible cellulosic biomass. The microorganisms are also capable of expressing an amylase, e.g., glucoamylase, having nonnegligible enzymatic activity, capable of producing glucose from oligo- or polysaccharides obtained by treating soluble starch with a-amylase. In some embodiments, nucleotidic material is provided comprising genes actively expressing xylose reductase, xylitol dehydrogenase and xylulokinase, as well as an active gene expressing glucoamylase. Vectors and other compositions of matter are provided as well.

    摘要翻译： 提供的是微生物，例如酵母酵母，其能够将从水解植物纤维素生物质获得的木糖从树木，草，秸秆等共同发酵得到葡萄糖，葡萄糖可以通过水解可食用的原料如 淀粉，蔗糖等，或从各种不可食用的纤维素生物质水解纤维素。 微生物还能够表达具有不可忽略的酶活性的淀粉酶，例如葡糖淀粉酶，其能够通过用α-淀粉酶处理可溶性淀粉而获得的寡糖或多糖产生葡萄糖。 在一些实施方案中，提供核苷酸材料，其包含主动表达木糖还原酶，木糖醇脱氢酶和木酮糖激酶的基因以及表达葡糖淀粉酶的活性基因。 还提供载体和其它物质组合物。

    NEW SACCHAROMYCES CEREVISIAE STRAINS
    10.
    发明申请
    NEW SACCHAROMYCES CEREVISIAE STRAINS 审中-公开
    新的沙门氏菌菌株

    公开(公告)号：WO2012067572A1

    公开(公告)日：2012-05-24

    申请号：PCT/SE2011/051369

    申请日：2011-11-15

    申请人： SCANDINAVIAN TECHNOLOGY GROUP AB ,  ALBERS, Eva ,  OLSSON, Lisbeth ,  KOPPRAM, Rakesh

    发明人： ALBERS, Eva ,  OLSSON, Lisbeth ,  KOPPRAM, Rakesh

    IPC分类号： C12N15/01 ,  C12N1/36 ,  C12N1/19 ,  C12P7/10 ,  C12R1/865

    CPC分类号： C12P7/06 ,  C12N9/0006 ,  C12N9/1205 ,  C12N15/01 ,  C12N15/81 ,  C12P7/10 ,  C12Q1/04 ,  C12R1/865 ,  C12Y101/01009 ,  C12Y101/0101 ,  C12Y101/01307 ,  C12Y207/01017 ,  Y02E50/16 ,  Y02E50/17

    摘要： A method for producing astrain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion and reduced xylitol production is described. The method comprises culturing the cells in a repetitive batch series in a medium at a xylose concentration of about 15-25 g/l and at a temperature of about 28- 32°C, and thereafter lowering the xylose concentration in at least one step at a temperature of about 28-32°C to obtain an increased selection pressure for improved xylose fermentation,improved ethanol production and reduced xylitol production, and continuing the culturing of the cells in said repetitive batch series. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.

    摘要翻译： 描述了用引入的木糖还原酶，木糖醇脱氢酶和木酮糖激酶基因产生酿酒酵母的方法，并且改进了乙醇生产，改善了木糖转化和降低的木糖醇生产。 该方法包括以约15-25g / l的木糖浓度和约28-32℃的温度在培养基中以重复批次序列培养细胞，然后在至少一步中降低木糖浓度 温度为约28-32℃，以获得用于改进的木糖发酵，改善的乙醇生产和减少的木糖醇生产的增加的选择压力，并且在所述重复批次系列中继续培养细胞。 此外，描述通过根据本发明的方法获得的酿酒酵母菌株。