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    SYSTEM AND PROCESS FOR BIOPOLYMER CHROMATOGRAPHY
    2.
    发明申请
    SYSTEM AND PROCESS FOR BIOPOLYMER CHROMATOGRAPHY 审中-公开
    用于生物聚合物色谱的系统和方法

    公开(公告)号：WO2012074481A1

    公开(公告)日：2012-06-07

    申请号：PCT/SE2011/051468

    申请日：2011-12-02

    Applicant: GE HEALTHCARE BIO-SCIENCES AB ,  HALL, Martin ,  LACKI, Karol

    Inventor： HALL, Martin ,  LACKI, Karol

    IPC: B01D15/18 ,  G01N30/44 ,  G01N30/46

    CPC classification number: B01D15/18 ,  B01D15/1871 ,  B01D15/242 ,  B01D15/3809 ,  B01D15/3847 ,  G01N30/42 ,  G01N30/44 ,  G01N30/465 ,  G01N2030/8831

    Abstract: A chromatography system (1 ) for separation of a biopolymer is described, comprising at least one feed tank (3), at least one hold tank (4; 4a, 4b, 4c), at least one elution buffer tank (5), at least one eluate tank (6), at least two packed bed chromatography columns (7,8) and for each packed bed chromatography column (7,8) at least one pump 810) and at least one outlet detector (11) both connected to said each packed bed chromatography column (7,8), wherein the feed tank, the hold tank(s), the elution buffer tank and the eluate tank are each connected to the packed bed chromatography columns via a system of valves (12), and wherein said hold tank(s) is/are connected to at least one inlet end (13) of a column (7,8) and at least one outlet end (14) of a column (7,8).

    Abstract translation: 描述了用于分离生物聚合物的色谱系统（1），其包括至少一个进料罐（3），至少一个储罐（4; 4a，4b，4c），至少一个洗脱缓冲罐（5） 至少一个洗脱液罐（6），至少两个填充床层析柱（7,8）和每个填充床层析柱（7,8）至少一个泵810）和至少一个出口检测器（11） 所述每个填充床色谱柱（7,8），其中所述进料罐，所述保持槽，所述洗脱缓冲罐和所述洗脱液罐各自通过阀（12）的系统连接到所述填充床色谱柱， 并且其中所述保持箱被连接到柱（7,8）的至少一个入口端（13）和柱（7,8）的至少一个出口端（14）。

    PRECIPITATION OF BIOMOLECULES WITH NEGATIVELY CHARGED POLYMERS
    4.
    发明申请
    PRECIPITATION OF BIOMOLECULES WITH NEGATIVELY CHARGED POLYMERS 审中-公开
    用负电荷聚合物降解生物分子

    公开(公告)号：WO2010082894A1

    公开(公告)日：2010-07-22

    申请号：PCT/SE2010/050017

    申请日：2010-01-11

    Applicant: GE HEALTHCARE BIO-SCIENCES AB ,  VAN ALSTINE, James ,  SHANAGAR, Jamil ,  HJORTH, Rolf ,  LACKI, Karol

    Inventor： VAN ALSTINE, James ,  SHANAGAR, Jamil ,  HJORTH, Rolf ,  LACKI, Karol

    IPC: C07K1/32 ,  B01D15/08 ,  B01D21/01 ,  C07K16/00

    CPC classification number: A61K39/39591 ,  B01D15/327 ,  B01D15/362 ,  B01D15/3809 ,  B01D15/3847 ,  C07K1/32 ,  C07K1/36

    Abstract: The present invention relates to methods of isolating biomolecules. More particularly, the invention relates to methods for isolating antibodies (mAbs) and related proteinsincluding antibody fragments (Fabs) under conditions where they are positive and relatively hydrophobic and will react with negatively charged polymer to form polymer-protein complexes which precipitate. The isolation can be accomplished usinginexpensive and biocompatible negatively charged polymers such as polyacrylic acid or carboxymethyldextran polymers of various molecular weights as precipitant.Itoccurs at relatively high concentrations of polymer (e.g. 10%) and high salt concentration (>50mM) and conductivity (e.g. >10 mS/cm) over wide range of pH.

    Abstract translation: 本发明涉及分离生物分子的方法。 更具体地，本发明涉及在阳性和相对疏水的条件下分离抗体（mAb）和相关蛋白（包括抗体片段（Fab））的方法，并且将与带负电荷的聚合物反应形成沉淀的聚合物 - 蛋​​白复合物。 可以使用密封性和生物相容性的带负电荷的聚合物来实现分离，如聚丙烯酸或各种分子量的羧甲基葡聚糖聚合物作为沉淀剂。在较高浓度的聚合物（例如10％）和高盐浓度（> 50mM）和电导率（例如> 10 mS / cm）。

    CHROMATOGRAPHY METHOD
    6.
    发明申请
    CHROMATOGRAPHY METHOD 审中-公开
    色谱法

    公开(公告)号：WO2008153472A1

    公开(公告)日：2008-12-18

    申请号：PCT/SE2008/000393

    申请日：2008-06-12

    Applicant: GE HEALTHCARE BIO-SCIENCES AB ,  BRYNTESSON, Mattias ,  HALL, Martin ,  LACKI, Karol

    Inventor： BRYNTESSON, Mattias ,  HALL, Martin ,  LACKI, Karol

    IPC: B01D15/18 ,  G01N30/46

    CPC classification number: B01D15/1828 ,  B01D15/1864 ,  B01D15/203 ,  G01N30/02 ,  G01N30/461 ,  G01N30/467 ,  G01N30/468 ,  G01N30/88 ,  G01N2030/8827 ,  G01N2030/8831 ,  G01N2030/8886 ,  B01D15/1821

    Abstract: The present invention relates to a simulated moving bed process, wherein at least one adsorbent is washed after binding of target compound and wherein the outlet of wash liquid from said adsorbent is subsequently passed onto another adsorbent for binding of target compound removed by said washing. In one embodiment, the method comprises binding of at least one target compound using three or more adsorbents connected in series and elution of target compound from said three adsorbents. After the binding to an adsorbent, wash liquid is passed across said adsorbent to recover desorbed and/or unbound target compound, and the outlet of such wash liquid is directed to the adsorbent after the next in the series, to which no feed has yet been added. The target compound is recovered by eluting target compound from the washed adsorbents.

    Abstract translation: 本发明涉及一种模拟移动床方法，其中在目标化合物结合之后洗涤至少一种吸附剂，并且其中来自所述吸附剂的洗涤液的出口随后通过另一种吸附剂，用于通过所述洗涤除去的目标化合物的结合。 在一个实施方案中，该方法包括使用三个或更多个串联连接的吸附剂和从所述三个吸附剂中洗脱目标化合物来结合至少一种目标化合物。 在结合到吸附剂之后，洗涤液体通过所述吸附剂以回收解吸和/或未结合的目标化合物，并且这种洗涤液体的出口在序列中的下一个之后被引导至吸附剂，其中还没有进料 添加。 通过从洗涤的吸附剂洗脱目标化合物来回收目标化合物。

    CHROMATOGRAPHY SYSTEM WITH GUARD COLUMNS
    7.
    发明申请
    CHROMATOGRAPHY SYSTEM WITH GUARD COLUMNS 审中-公开
    具有保护色谱柱的色谱系统

    公开(公告)号：WO2012057676A1

    公开(公告)日：2012-05-03

    申请号：PCT/SE2011/051254

    申请日：2011-10-24

    Applicant: GE HEALTHCARE BIO-SCIENCES AB ,  LACKI, Karol

    Inventor： LACKI, Karol

    IPC: G01N30/46 ,  B01D15/18 ,  G01N30/60 ,  G01N30/88

    CPC classification number: G01N30/461 ,  B01D15/1871 ,  B01D15/3809 ,  G01N2030/8813

    Abstract: A chromatography system with a main column (1) comprising a chromatography resin, a first guard column (2) and a second guard column (3), wherein the first guard column (2) is connected to a first end of the main column (4), the second guard column (3) is connected to a second end (5) of the main column and the bed volumes of said first and second guard columns are each less than about 50% of the bed volume of the main column.

    Abstract translation: 一种色谱系统，其具有主色谱柱（1），其包括色谱树脂，第一保护柱（2）和第二保护柱（3），其中所述第一保护柱（2）连接到所述主色谱柱的第一端 如图4所示，第二保护柱（3）连接到主柱的第二端（5），并且所述第一和第二保护柱的床体积分别小于主塔的床体积的约50％。

    A METHOD IN A CHROMATOGRAPHY SYSTEM
    8.
    发明申请
    A METHOD IN A CHROMATOGRAPHY SYSTEM 审中-公开
    色谱系统中的方法

    公开(公告)号：WO2010151214A1

    公开(公告)日：2010-12-29

    申请号：PCT/SE2010/050700

    申请日：2010-06-21

    Applicant: GE HEALTHCARE BIO-SCIENCES AB ,  BÄNGTSSON, Petra ,  ESTRADA, Erik ,  LACKI, Karol ,  SKOGLAR, Helena

    Inventor： BÄNGTSSON, Petra ,  ESTRADA, Erik ,  LACKI, Karol ,  SKOGLAR, Helena

    IPC: G01N30/86 ,  B01D15/14

    CPC classification number: G01N30/88 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/428 ,  G01N30/42 ,  G01N30/468 ,  G01N30/78 ,  G01N30/8665 ,  G01N2030/889

    Abstract: A method for determining binding capacities of a chromatography column (1; 39, 47, 59; 107, 109, 111, 113), comprising: - detecting a feed signal (21; 201) representative of the composition of a feed material provided to the inlet of the column; - detecting an effluent signal (23; 203, 205, 207, 209) representative of the composition of the effluent from the column; - using the feed signal and the effluent signal to determine binding capacities of the column. And a method for controlling a chromatography system comprising at least one column, comprising the steps of: - determining binding capacities of the at least one chromatography column according to above; and - controlling the start and stop of the different chromatography process steps according to the determined binding capacities.

    Abstract translation: 一种用于确定色谱柱（1; 39,47,59; 107,109,111,113）的结合能力的方法，包括： - 检测代表供给材料的组成的进料信号（21; 201） 柱的入口; - 检测代表来自塔的流出物的组成的流出物信号（23; 203,205,207,209）; - 使用进料信号和流出物信号来确定塔的结合能力。 以及用于控制包含至少一个柱的色谱系统的方法，包括以下步骤： - 测定根据上述的至少一个色谱柱的结合能力; 以及 - 根据确定的结合能力控制不同色谱法步骤的开始和停止。

    AQUEOUS TWO PHASE EXTRACTION AUGMENTED PRECIPITATION PROCESS FOR PURIFICATION OF THERAPEUTIC PROTEINS
    9.
    发明申请
    AQUEOUS TWO PHASE EXTRACTION AUGMENTED PRECIPITATION PROCESS FOR PURIFICATION OF THERAPEUTIC PROTEINS 审中-公开
    水溶性两相提取增效降解方法，用于净化治疗蛋白

    公开(公告)号：WO2010062244A1

    公开(公告)日：2010-06-03

    申请号：PCT/SE2009/051305

    申请日：2009-11-18

    Applicant: GE HEALTHCARE BIO-SCIENCES AB ,  TRAN, Richard ,  TITCHENER-HOOKER, Nigel, J. ,  LACKI, Karol

    Inventor： TRAN, Richard ,  TITCHENER-HOOKER, Nigel, J. ,  LACKI, Karol

    IPC: C07K1/36 ,  C07K1/14 ,  C07K1/30 ,  C07K16/00

    CPC classification number: C07K1/30 ,  C07K1/145 ,  C07K1/36 ,  C07K16/065

    Abstract: The invention relates to an aqueous two phase extraction (ATPE) augmented precipitation process, which may be used to recover and also partially purify therapeutic proteins, including monoclonal antibodies from a crude multi-component mixture. The process involves the formation of a forward extraction PEG-Phosphate ATPE system in which the target product is preferentially partitioned to the polymer rich phase. A second ATPE back extraction system is then formed by introducing the polymer rich phase from the forward extraction to a new phosphate salt rich phase, causing the product to precipitate at the interface between the two phases. This precipitate is then recovered and resolubilised in a suitable buffer and may be passed on for further purification.

    Abstract translation: 本发明涉及水相两相提取（ATPE）增强沉淀方法，其可用于回收并且还部分纯化来自粗多组分混合物的治疗性蛋白质，包括单克隆抗体。 该方法包括形成正向提取的PEG-磷酸酯ATPE系统，其中目标产物优选分配到富聚合物相。 然后通过将富含聚合物的相从前向萃取引入富磷相，形成第二个ATPE反萃取系统，使产物在两相之间的界面沉淀。 然后将该沉淀物回收并在合适的缓冲液中再溶解，并且可以通过以进一步纯化。

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