公开(公告)号：WO2009024862A2

公开(公告)日：2009-02-26

申请号：PCT/IB2008/002573

申请日：2008-08-14

申请人： DANISCO A/S ,  LARSEN, Neils, Erik ,  SØE, Jørn, Borch

发明人： LARSEN, Neils, Erik ,  SØE, Jørn, Borch

IPC分类号： A23C19/032 ,  C12Q1/48 ,  C07K14/195 ,  C12P7/64 ,  C12N9/10 ,  A21D8/04

CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23C19/0684 ,  A23L15/25 ,  A23L29/06 ,  C12N9/1029 ,  C12N9/18 ,  C12N11/00 ,  C12Y301/01

摘要： The use of a lipid acyltransferase in the manufacture of UHT milk for improving the stability, particularly the long term stability, and/or improving the perceptible sensory difference and/or improving smell and/or taste and/or for reducing the cholesterol content and/or for eliminating or reducing creaming of the UHT milk for reducing the cholesterol content in the UHT milk. A method of producing UHT milk, wherein method comprises admixing a lipid acyltransferase and milk (including a step of processing the milk to make it a UHT milk). Preferably said lipid acyltranferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridises under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ ID No. 49; and/or is a polypeptide having lipid acyltranferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence sequence which as has 70% or more identity therewith.

摘要翻译： 脂质酰基转移酶在制造UHT乳中用于改善稳定性，特别是长期稳定性和/或改善可察觉的感觉差异和/或改善气味和/或味道和/或降低胆固醇含量和/ 或用于消除或减少UHT牛奶的奶油以降低UHT牛奶中的胆固醇含量。 一种生产UHT奶的方法，其中方法包括混合脂质酰基转移酶和乳（包括加工牛奶以使其成为UHT牛奶的步骤）。 优选地，所述脂质酰基转移酶是具有脂质酰基转移酶活性的多肽，其多肽通过表达如SEQ ID No.49所示的核苷酸序列或与其同一性具有70％或更多同一性的核苷酸序列获得; 和/或通过在中等严格条件下与包含SEQ ID No.49所示的核苷酸序列的核酸探针杂交的核酸的表达获得; 和/或是具有脂质酰基转移酶活性的多肽，该多肽包含SEQ ID No.68所示的氨基酸序列或与其同一性具有70％以上的氨基酸序列。

公开(公告)号：WO2009024862A3

公开(公告)日：2009-05-22

申请号：PCT/IB2008002573

申请日：2008-08-14

申请人： DANISCO ,  LARSEN NEILS ERIK ,  SOEE JOERN BORCH

发明人： LARSEN NEILS ERIK ,  SOEE JOERN BORCH

IPC分类号： A23C19/032 ,  A21D8/04 ,  C07K14/195 ,  C12N9/10 ,  C12P7/64 ,  C12Q1/48

CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23C19/0684 ,  A23L15/25 ,  A23L29/06 ,  C12N9/1029 ,  C12N9/18 ,  C12N11/00 ,  C12Y301/01

摘要： The use of a lipid acyltransferase in the manufacture of UHT milk for improving the stability, particularly the long term stability, and/or improving the perceptible sensory difference and/or improving smell and/or taste and/or for reducing the cholesterol content and/or for eliminating or reducing creaming of the UHT milk for reducing the cholesterol content in the UHT milk. A method of producing UHT milk, wherein method comprises admixing a lipid acyltransferase and milk (including a step of processing the milk to make it a UHT milk). Preferably said lipid acyltranferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridises under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ ID No. 49; and/or is a polypeptide having lipid acyltranferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence sequence which as has 70% or more identity therewith.

摘要翻译： 脂质酰基转移酶在制造UHT乳中用于改善稳定性，特别是长期稳定性，和/或改善可察觉的感觉差异和/或改善气味和/或味道和/或降低胆固醇含量和/ 或用于消除或减少超高温处理牛奶的分层以降低超高温灭菌牛奶中的胆固醇含量。 一种生产超高温灭菌乳的方法，其中方法包括混合脂质酰基转移酶和乳（包括处理乳以使其成为超高温灭菌乳的步骤）。 优选地，所述脂质酰基转移酶是具有脂质酰基转移酶活性的多肽，所述多肽通过表达SEQ ID No.49所示的核苷酸序列或与其具有70％或更多同一性的核苷酸序列获得; 和/或通过表达在中等严格条件下与包含如SEQ ID No.49所示核苷酸序列的核酸探针杂交的核酸获得; 和/或是具有脂质酰基转移酶活性的多肽，该多肽包含如SEQ ID No.68所示的氨基酸序列或与其具有70％或更多同一性的氨基酸序列序列。