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    PRODUCTION OF CHEESE WITH S. THERMOPHILUS
    1.
    发明申请
    PRODUCTION OF CHEESE WITH S. THERMOPHILUS 审中-公开
    与S.热带植物的生产

    公开(公告)号：WO2012145629A2

    公开(公告)日：2012-10-26

    申请号：PCT/US2012/034455

    申请日：2012-04-20

    Applicant: DANISCO A/S ,  PETERSEN, Lars, Wexoe

    Inventor： PETERSEN, Lars, Wexoe

    IPC: A23C19/032 ,  A23C19/05 ,  A23C19/068 ,  A23C19/072 ,  C12R1/46

    CPC classification number: A23C19/076 ,  A23C9/13 ,  A23C19/032 ,  A23C19/0323 ,  A23C19/051 ,  A23C19/054 ,  A23C19/068 ,  A23C19/0684 ,  A23C19/0688 ,  A23C19/072 ,  A23Y2240/75 ,  C12N1/20 ,  C12R1/46

    Abstract: Methods and compositions for producing cheese with S. thermophilus and a urease inhibitor, and for producing hard or semi-hard cheese, such as cheddar, with S. thermophilus that is partially or completely deficient in its ability to release ammonia from urea are provided. Methods and compositions for reducing, the amount of open texture (e.g., slits, cracks, or fractures) in hard and semi-hard cheeses, as well as hard and semi-hard cheeses that comprise one or more S. thermophilus bacteria that are partially or completely deficient in their ability to release ammonia from urea, are also provided.

    Abstract translation: 提供了用嗜热链球菌和脲酶抑制剂生产奶酪的方法和组合物，并且用于生产硬或半硬干酪例如切达干酪，其中嗜热链球菌部分或完全缺乏其从尿素释放氨的能力。 用于减少硬和半硬质奶酪中的开放纹理（例如狭缝，裂缝或裂缝）的量以及包含一种或多种部分嗜热链球菌的硬和半硬干酪的方法和组合物 或完全缺乏其从尿素释放氨的能力。

    PRODUCTION OF CHEESE WITH S. THERMOPHILUS
    2.
    发明申请
    PRODUCTION OF CHEESE WITH S. THERMOPHILUS 审中-公开
    与S.热带植物的生产

    公开(公告)号：WO2012145047A1

    公开(公告)日：2012-10-26

    申请号：PCT/US2012/021113

    申请日：2012-01-12

    Applicant: DANISCO A/S ,  PETERSEN, Lars Wexoe

    Inventor： PETERSEN, Lars Wexoe

    IPC: A23C19/032 ,  A23C19/076 ,  C12R1/46

    CPC classification number: A23C19/076 ,  A23C9/13 ,  A23C19/032 ,  A23C19/0323 ,  A23C19/051 ,  A23C19/054 ,  A23C19/068 ,  A23C19/0684 ,  A23C19/0688 ,  A23C19/072 ,  A23Y2240/75 ,  C12N1/20 ,  C12R1/46

    Abstract: The present invention provides methods, compositions, and systems for producing cheese with S. thermophilus and a urease inhibitor, and for producing cottage cheese with S. thermophilus that is partially or completely deficient in its ability to release ammonia from urea. The present invention also provides methods, compositions, and systems for reducing the amount of open texture (e.g., slits, cracks, or fractures) in gassy cheeses, such as cheddar cheese.

    Abstract translation: 本发明提供了用嗜热链球菌和脲酶抑制剂生产奶酪的方法，组合物和系统，以及用嗜热链球菌生产山羊奶酪，其部分或完全缺乏其从尿素中释放氨的能力。 本发明还提供了用于减少诸如切达干酪之类的高档奶酪中的开放纹理（例如狭缝，裂缝或裂缝）的量的方法，组合物和系统。

    METHOD
    3.
    发明申请
    METHOD 审中-公开
    方法

    公开(公告)号：WO2012004759A2

    公开(公告)日：2012-01-12

    申请号：PCT/IB2011/053018

    申请日：2011-07-07

    Applicant: DANISCO A/S ,  ISAKSEN, Mai Faurschou

    Inventor： ISAKSEN, Mai Faurschou

    IPC: C12Q1/40

    CPC classification number: C12N9/2417 ,  A23K20/174 ,  A23K20/189 ,  A23K20/20 ,  A23K40/10 ,  A23K40/25 ,  A23K40/30 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  C07K2319/02 ,  C12Q1/40 ,  C12Y302/01001 ,  Y02A40/818 ,  Y02P60/877

    Abstract: The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with corn based feed and buffer solution comprising a pepsin concentration of 9000 U/ml at pH 3, 40°C, 500 rpm for at least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feedstuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.

    Abstract translation: 本发明涉及用于鉴定用于饲料添加物的胃蛋白酶抗性α淀粉酶的方法，其包括：i）提供α淀粉酶; ii）将所述α淀粉酶与基于玉米的饲料和缓冲溶液混合，所述饲料和缓冲溶液在pH 3,40℃，500rpm下包含9000U / ml的胃蛋白酶浓度至少120分钟，并且与对照组相比分析所述α淀粉酶上的α淀粉酶活性 样品; 其中所述对照样品的差异在于孵育期间不存在胃蛋白酶; 和iii）选择在测定条件下基本上保持α-淀粉酶活性的α淀粉酶; 饲料添加剂和饲料，其包含胃蛋白酶抗性α淀粉酶和在饲料中使用胃蛋白酶抗性α淀粉酶。

    SEPARATION PROCESS
    5.
    发明申请
    SEPARATION PROCESS 审中-公开
    分离过程

    公开(公告)号：WO2011121179A1

    公开(公告)日：2011-10-06

    申请号：PCT/FI2011/050262

    申请日：2011-03-29

    Applicant: DANISCO A/S ,  AIRAKSINEN, Jyrki ,  PAANANEN, Hannu ,  LEWANDOWSKI, Jari ,  LAIHO, Kari

    Inventor： AIRAKSINEN, Jyrki ,  PAANANEN, Hannu ,  LEWANDOWSKI, Jari ,  LAIHO, Kari

    IPC: B01D15/18 ,  C13B20/14 ,  C13B35/06 ,  C07C229/12

    CPC classification number: C13B20/148 ,  B01D15/185 ,  C07C227/40 ,  C13K13/00 ,  C07C229/12

    Abstract: The invention relates to a multi-profile chromatographic sequential SMB process of separating and recovering betaine and sucrose from concentrated raw juice or thick juice. In the process of the invention, the betaine subprofile and the residual subprofile of successive separation profiles are overlapping. A sucrose fraction and a betaine fraction containing also residual components are recovered. Betaine is further separated from the betaine fraction for example by chromatographic processes.

    Abstract translation: 本发明涉及从浓缩的原汁或浓稠汁中分离和回收甜菜碱和蔗糖的多色谱色谱顺序SMB方法。 在本发明的过程中，甜菜碱子档案和连续分离谱的残留子档案重叠。 回收含有残留成分的蔗糖级分和甜菜碱级分。 甜菜碱例如通过色谱法从甜菜碱级分进一步分离。

    PROTEIN
    7.
    发明申请
    PROTEIN 审中-公开
    蛋白

    公开(公告)号：WO2010150213A1

    公开(公告)日：2010-12-29

    申请号：PCT/IB2010/052868

    申请日：2010-06-23

    Applicant: DANISCO A/S ,  MIASNIKOV, Andrei ,  BOTT, Richard, R ,  SØRENSEN, Jens, Frisbæk

    Inventor： MIASNIKOV, Andrei ,  BOTT, Richard, R ,  SØRENSEN, Jens, Frisbæk

    IPC: C12N9/18 ,  C12N9/20 ,  A23L1/015 ,  C12N15/55

    CPC classification number: C12N9/20 ,  A21D8/042 ,  C12N9/18 ,  C12Y301/01003 ,  C12Y301/01004 ,  C12Y301/01026 ,  C12Y301/01032

    Abstract: The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site (or one additional glycosylation site) in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position on the polypeptide and at a location in an external loop distal to the active site (catalytic triad) of the enzyme which is more hydrophilic (compared with the original amino acid); or c) a substitution or insertion at one or more of positions 33, 63, 78, 190, 305, 306 or 320 or a deletion at one or more positions 311-312 or 307-319, wherein each amino acid position corresponds to the position of the amino acid sequence when aligned with SEQ ID No. 2; wherein when the nucleotide sequence has at least 90% identity with a nucleotide sequence encoding the fungal lipolytic enzyme shown in SEQ ID No. 22 or SEQ ID No. 23 the modification is not a substitution at position 63 and the deletion is not at position 311 -312. Preferably the nucleotide sequence has at least 90% identity with SEQ ID No. 1. The invention also relates to polypeptide produced by the method and to novel nucleic acids.

    Abstract translation: 本发明涉及一种制备变体脂肪分解酶的方法，其包括在宿主生物体中表达与编码真菌脂肪分解酶的核苷酸序列具有至少90％同一性的核苷酸序列，并且包括在对应的位置处的至少一个修饰 在编码的氨基酸序列中a）与原始真菌脂肪分解酶相比，在氨基酸序列中引入至少一个糖基化位点（或另外一个糖基化位点）; b）在多肽的表面位置和远离更亲水的酶（与原始氨基酸相比）的活性位点（催化三单元组）的外部环路的位置处引入至少一个氨基酸; 或c）在位置33,63,78,190,305,306或320中的一个或多个处的取代或插入或在一个或多个位置311-312或307-319处的缺失，其中每个氨基酸位置对应于 当与SEQ ID No.2对齐时，氨基酸序列的位置; 其中当所述核苷酸序列与编码SEQ ID No.22或SEQ ID No.23所示的真菌脂肪分解酶的核苷酸序列具有至少90％同一性时，所述修饰不是位置63处的取代，并且缺失不在位置311 -312。 优选地，核苷酸序列与SEQ ID No.1具有至少90％的同一性。本发明还涉及通过该方法产生的多肽和新的核酸。

    METHOD
    10.
    发明申请
    METHOD 审中-公开
    方法

    公开(公告)号：WO2010122531A2

    公开(公告)日：2010-10-28

    申请号：PCT/IB2010/051802

    申请日：2010-04-23

    Applicant: DANISCO A/S ,  MADRID, Susan M ,  LIN, Cherry ,  ZARGAHI, Masoud Rajabi ,  LORENTSEN, Rikke Høegh ,  ISAKSEN, Mai Faurschou ,  WARD, Michael

    Inventor： MADRID, Susan M ,  LIN, Cherry ,  ZARGAHI, Masoud Rajabi ,  LORENTSEN, Rikke Høegh ,  ISAKSEN, Mai Faurschou ,  WARD, Michael

    IPC: C12N9/20

    CPC classification number: C12N9/20 ,  A23K20/189 ,  A23L29/06 ,  A23L33/14 ,  A23V2002/00 ,  C12N15/80 ,  C12Y301/01003

    Abstract: The present invention provides a method of producing a lipolytic enzyme comprising the steps of: (i) providing a Trichoderma reesei cell comprising a) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 or an amino acid sequence which has at least 40% sequence identity to SEQ ID NO: 1 or 2; and/or b) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4, and/or c) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which has at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4 or the complement of any thereof under stringent conditions; and (ii) culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.

    Abstract translation: 本发明提供一种生产脂肪分解酶的方法，包括以下步骤：（i）提供里氏木霉细胞，其包含a）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1所示的氨基酸序列或 SEQ ID NO：2或与SEQ ID NO：1或2具有至少40％序列同一性的氨基酸序列; 和/或b）编码脂解酶的至少一种异源核苷酸序列，其中所述核苷酸序列包含SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列 SEQ ID NO：3或SEQ ID NO：4，和/或c）至少一个编码脂肪分解酶的异源核苷酸序列，其中所述核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或 在严格条件下与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性或其任何互补序列的核苷酸序列; 和（ii）在允许表达编码所述脂肪分解酶的所述异源核苷酸序列的条件下培养细胞。

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