公开(公告)号：WO2007024735A3

公开(公告)日：2007-11-01

申请号：PCT/US2006032443

申请日：2006-08-21

申请人： CHARM SCIENCES INC ,  LAW SAY-JONG ,  SAUL STEVEN J ,  CHARM STANLEY E

发明人： LAW SAY-JONG ,  SAUL STEVEN J ,  CHARM STANLEY E

IPC分类号： C07K16/00 ,  C07K1/04 ,  C07K1/10 ,  C07K1/13 ,  G01N37/00

CPC分类号： G01N33/9446 ,  C07K16/44

摘要： An antibody and immunogen for generating an antibody to nitrofuran and/or nitrofuran metabolite such as tissue or protein bound nitrofuran metabolites. Nitrofurans and and/or nitrofuran metabolites in a biological sample can be detected by contacting the sample with the antibodies to form a complex that can be detected. The antibodies may also be incorporated into test kits for the detection of nitrofuran and/or nitrofuran metabolites.

摘要翻译： 用于产生硝基呋喃和/或硝基呋喃代谢物的抗体和免疫原，例如组织或蛋白质结合的硝基呋喃代谢物。 通过使样品与抗体接触以形成可检测的复合物，可以检测生物样品中的硝基呋喃和/或硝基呋喃代谢物。 抗体也可以并入用于检测硝基呋喃和/或硝基呋喃代谢物的检测试剂盒中。

公开(公告)号：WO2005103698A3

公开(公告)日：2005-11-03

申请号：PCT/US2005/012898

申请日：2005-04-15

申请人： CHARM SCIENCES, INC. ,  LAW, Say-Jong ,  CHARM, Stanley, E. ,  SAUL, Steven, J.

发明人： LAW, Say-Jong ,  CHARM, Stanley, E. ,  SAUL, Steven, J.

IPC分类号： C07K16/44 ,  C07K17/02 ,  C07K17/06 ,  G01N33/53 ,  G01N33/531 ,  C07D413/06 ,  C07D236/04 ,  C07D233/46

摘要： An antibody and immunogen for generating an antibody to nitrofuran and/or nitrofuran metabolite such as tissue or protein bound nitrofuran metabolites. Nitrofurans and and/or nitrofuran metabolites in a biological sample can be detected by contacting the sample with the antibodies to form a complex that can be detected. The antibodies may also be incorporated into test kits for the detection of nitrofuran and/or nitrofuran metabolites.

公开(公告)号：WO2005103698A2

公开(公告)日：2005-11-03

申请号：PCT/US2005012898

申请日：2005-04-15

申请人： CHARM SCIENCES INC ,  LAW SAY-JONG ,  CHARM STANLEY E ,  SAUL STEVEN J

发明人： LAW SAY-JONG ,  CHARM STANLEY E ,  SAUL STEVEN J

IPC分类号： C07K16/44 ,  G01N33/535 ,  G01N33/94

CPC分类号： G01N33/9446 ,  C07K16/44

摘要： An antibody and immunogen for generating an antibody to nitrofuran and/or nitrofuran metabolite such as tissue or protein bound nitrofuran metabolites. Nitrofurans and and/or nitrofuran metabolites in a biological sample can be detected by contacting the sample with the antibodies to form a complex that can be detected. The antibodies may also be incorporated into test kits for the detection of nitrofuran and/or nitrofuran metabolites.

摘要翻译： 用于产生硝基呋喃和/或硝基呋喃代谢物的抗体和免疫原，例如组织或蛋白质结合的硝基呋喃代谢物。 通过使样品与抗体接触以形成可检测的复合物，可以检测生物样品中的硝基呋喃和/或硝基呋喃代谢物。 抗体也可以并入用于检测硝基呋喃和/或硝基呋喃代谢物的检测试剂盒中。

公开(公告)号：WO9854574A3

公开(公告)日：1999-03-04

申请号：PCT/IB9800831

申请日：1998-05-29

申请人： CHIRON DIAGNOSTICS CORP ,  JIANG QINGPING ,  XI JUN ,  NATRAJAN ANAND ,  SHARPE DAVID ,  BAUMANN MARCUS ,  HILFIKER ROLF ,  SCHMIDT ERIKA ,  SENN PAUL ,  THOMMEN FRITZ ,  WALDNER ADRIAN ,  ALDER ALEX ,  LAW SAY JONG

发明人： JIANG QINGPING ,  XI JUN ,  NATRAJAN ANAND ,  SHARPE DAVID ,  BAUMANN MARCUS ,  HILFIKER ROLF ,  SCHMIDT ERIKA ,  SENN PAUL ,  THOMMEN FRITZ ,  WALDNER ADRIAN ,  ALDER ALEX ,  LAW SAY-JONG

IPC分类号： C09B11/04 ,  C09B15/00 ,  C09K11/07 ,  G01N33/533 ,  G01N33/542 ,  G01N33/58

CPC分类号： C09B15/00 ,  C09B11/04 ,  G01N33/533 ,  G01N33/542 ,  G01N33/582 ,  Y10S436/80 ,  Y10S436/816 ,  Y10S436/817

摘要： A new class of chemiluminescent acridinium or benzacridinium compounds is disclosed by virtue of forming an intramolecular energy transfer conjugate (ETC) between the acridinium or benzacridinium compound and a luminophore. A method of extending the emission wavelengths of acridinium or benzacridinium esters in order to further reduce or eliminate the emission spectral overlap between the parent polysubstituted aryl Acridinium Esters (DMAE) and Benzacridinium Esters (LEAE) is disclosed. The ETC's retain the unique desired properties of acridinium or benzacridinium compounds including complete light emission in very short period of time, monophasic emission spectrum, simplicity of triggering mechanism, ability of labeling the biological molecules of interest to form a tracer, and good stability. Additionally, the range of the emission spectrum of an acridinium or benzacridinium compound can now be shifted at will and at longer leap through the choice of a luminophore as the integral part of an ETC molecule. Disclosed are chemiluminescent labeled conjugates comprising an acridinium or benzacridinium moiety convalently attached to a luminophore via a spacer, said moiety further conjugated to a biological molecule of interest, wherein said spacer is of an appropriate length to allow the excited species generated from said moiety to transfer energy efficiently to said luminophore, resulting in the emission of light in the spectral region of said luminophore. Also disclosed are binding assays using said conjugates, test kits comprising said conjugates and methods of preparing the conjugates.

公开(公告)号：WO2008115580A3

公开(公告)日：2008-09-25

申请号：PCT/US2008/003750

申请日：2008-03-21

申请人： CHARM SCIENCES, INC. ,  LAW, Say-Jong

发明人： LAW, Say-Jong

IPC分类号： G01N33/53 ,  G01N24/00 ,  C07K1/10 ,  C07K1/13 ,  C07K1/04 ,  C07K16/00

摘要： A method for detecting nitrofuran that includes rapid acid hydrolysis of sample to obtain a sample suitable for testing in less than 16 hours.

公开(公告)号：WO2008115580A2

公开(公告)日：2008-09-25

申请号：PCT/US2008003750

申请日：2008-03-21

申请人： CHARM SCIENCES INC ,  LAW SAY-JONG

发明人： LAW SAY-JONG

IPC分类号： G01N33/53 ,  C07K16/40

CPC分类号： G01N33/9446

摘要： A method for detecting nitrofuran that includes rapid acid hydrolysis of sample to obtain a sample suitable for testing in less than 16 hours.

摘要翻译： 一种检测硝基呋喃的方法，包括对样品进行快速酸水解，以获得适于在少于16小时内测试的样品。

公开(公告)号：WO2007024735A2

公开(公告)日：2007-03-01

申请号：PCT/US2006/032443

申请日：2006-08-21

申请人： CHARM SCIENCES, INC. ,  LAW, Say-jong ,  SAUL, Steven J. ,  CHARM, Stanley, E.

发明人： LAW, Say-jong ,  SAUL, Steven J. ,  CHARM, Stanley, E.

IPC分类号： G01N33/53 ,  C07K16/40

CPC分类号： G01N33/9446 ,  C07K16/44

摘要： An antibody and immunogen for generating an antibody to nitrofuran and/or nitrofuran metabolite such as tissue or protein bound nitrofuran metabolites. Nitrofurans and and/or nitrofuran metabolites in a biological sample can be detected by contacting the sample with the antibodies to form a complex that can be detected. The antibodies may also be incorporated into test kits for the detection of nitrofuran and/or nitrofuran metabolites.

摘要翻译： 用于产生针对硝基呋喃和/或硝基呋喃代谢物（例如组织或蛋白质）结合的硝基呋喃代谢物的抗体的抗体和免疫原。 生物样品中的硝基呋喃和/或硝基呋喃代谢物可以通过将样品与抗体接触以形成可检测的复合物来检测。 也可以将抗体掺入检测试剂盒中以检测硝基呋喃和/或硝基呋喃代谢物。

公开(公告)号：WO1995027702A1

公开(公告)日：1995-10-19

申请号：PCT/IB1995000244

申请日：1995-04-06

申请人： CIBA CORNING DIAGNOSTICS CORP. ,  LAW, Say-Jong ,  SOTIRIOU-LEVENTIS, Chariklia ,  NATRAJAN, Anand ,  JIANG, Qingping ,  CONNOLLY, Peter, B. ,  KILROY, John, P. ,  MCCUDDEN, Constance, R. ,  TIRRELL, Stephen, M.

发明人： CIBA CORNING DIAGNOSTICS CORP.

IPC分类号： C07D219/04

CPC分类号： C07D401/12 ,  C07D219/04 ,  C07F9/64 ,  C07J1/0011 ,  C07J1/0022 ,  C07J21/006 ,  C07J43/003 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/586 ,  C12Q2563/161 ,  C12Q2563/103

摘要： Novel acridinium esters that are useful, either alone or when incorporated into liposomes, as chemiluminescent agents in binding assays (e.g., immunoassays and gene probe assays) with improved sensitivity are disclosed. In addition, the synthesis of these esters and their use in assays for detecting an analyte is described. In particular, assays for testosterone and the Rubella virus are disclosed.

摘要翻译： 公开了单独使用或当掺入脂质体时作为具有改善的灵敏度的结合测定（例如免疫测定和基因探针测定）中的化学发光剂的新型吖啶酯。 此外，描述了这些酯的合成及其在用于检测分析物的测定中的用途。 特别地，公开了睾酮和风疹病毒的测定。

公开(公告)号：WO0109372B1

公开(公告)日：2001-06-14

申请号：PCT/US0020429

申请日：2000-07-27

申请人： BAYER AG ,  JIANG QINGPING ,  NATRAJAN ANAND ,  SHARPE DAVID J ,  WONG WEN JEE ,  LAW SAY JONG

发明人： JIANG QINGPING ,  NATRAJAN ANAND ,  SHARPE DAVID J ,  WONG WEN-JEE ,  LAW SAY-JONG

IPC分类号： G01N21/78 ,  C07D219/04 ,  C07D491/10 ,  C07F9/64 ,  C09K11/07 ,  C12M1/34 ,  C12Q1/34 ,  C12Q1/42 ,  C07D219/06

CPC分类号： C07D491/10 ,  C07D219/04 ,  C07F9/64 ,  C12Q1/42 ,  C12Q2334/00

摘要： Chemiluminescent substrates of hydrolytic enzymes are disclosed having general Formula Lumi-M-P, where Lumi is a chemiluminescent moiety capable of producing light (a) by itself, (b) with MP attached and (c) with M attached, wherein the different properties of Lumi-M-P and Lumi-M allow them to be distinguished. Lumi includes, but is not limited to, acridinium compounds (e.g. acridinium esters, carboxyamides, thioesters and oxime esters), reduced forms thereof (e.g. acridans), and spiroacridan compounds. M is selected from oxygen, nitrogen and sulfur. P is a group that can be readily removed by hydrolytic enzymes to give Lumi-M and P. The hydrolytic enzyme can be phosphatase, glycosidase, peptidase, protease, esterase, sulfatase and guanidinobenzoatase.

摘要翻译： 公开了水解酶的化学发光底物，其具有通式Lumi-MP，其中Lumi是能够自身产生光（a）的化学发光部分，（b）MP附着和（c）与M连接，其中Lumi的不同性质 -MP和Lumi-M允许他们被区别。 Lumi包括但不限于吖啶鎓化合物（例如吖啶酯，羧酰胺，硫酯和肟酯），还原形式（例如吖啶）和螺吡啶化合物。 M选自氧，氮和硫。 P是可以通过水解酶容易地除去得到Lumi-M和P的基团。水解酶可以是磷酸酶，糖苷酶，肽酶，蛋白酶，酯酶，硫酸酯酶和胍基苯甲酰化酶。

公开(公告)号：WO0009487A9

公开(公告)日：2000-06-22

申请号：PCT/US9918076

申请日：1999-08-10

申请人： BAYER AG ,  NATRAJAN ANAND ,  JIANG QINGPING ,  SHARPE DAVID ,  LAW SAY JONG

发明人： NATRAJAN ANAND ,  JIANG QINGPING ,  SHARPE DAVID ,  LAW SAY-JONG

IPC分类号： C07D219/04 ,  C07D401/12 ,  C07K14/765 ,  C07K16/26 ,  C09K3/00 ,  C09K11/07 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/76 ,  G01N33/532 ,  G01N33/58 ,  G01N33/533

CPC分类号： G01N33/582 ,  C07D219/04 ,  Y10S436/80 ,  Y10S436/815 ,  Y10S436/817 ,  Y10S530/812 ,  Y10T436/13

摘要： Our results thus identify two sets of necessary and sufficient criteria for observing long-wavelength emission from acridinium compounds: Set A: (a) the creation of an extended conjugation system by the attachment of appropriate functional groups on the acridinium nucleus (electronic requirement). (b) Coplanarity of the attached functional group and the acridone moiety during light emission (geometry requirement). (c) Said functional group must consist of at least one aromatic ring and one electron-donating atom or group with an extra pair of electrons which can readily delocalize into the extended pi system to which the heteroatom is directly attached or built into, and establish stable extended resonance with the electron-withdrawing carbonyl moiety of the light emitting acridone. Such electron-donating atom or group that exists in the form of an anion has particularly strong effect to further the bathochromic shift of the emission wavelength. Set B: (a) A direct attachment at one or more of positions C-2, C-4, C-5, or C-7 of the acridinium nucleus, of electron-donating atoms or groups having extra pair(s) of electrons. The electron-donating entities can be the same or different if more than one electron-donating entity is used. Such electron-donating atom or group that exists in the form of an anion has particularly strong effect to further the bathochromic shift of the emission wavelenth. For molecules for which the above criteria are met such as LEAE, 3-HS-DMAE, and 2-hydroxy-DMAE long wavelength-emission exceeding 500 nm and reaching into NIR region is expected and observed. Preferably, the utility of an NIR-AC of comparable quantum yield as the conventional acridinium compounds goes hand-in-hand with the employment of a luminescence detector of good to excellent detection efficiency. To achieve efficient NIR signal detection and facilitate the performing of diagnostic assays, a further objective of the present invention is the advance of a concept and the realization of substituting a state-of-the-art charge-coupled device (CCD) detector for the red-insensitive photomultiplier tube (PMT) in a conventional fully or semi-automatic analyzer such as MLA-II of Chiron Diagnostics, Walpole, MA.

摘要翻译： 因此，我们的结果确定了观察吖啶鎓化合物长波长发射的两套必要和充分的标准：A组：（a）通过在吖啶鎓核上附加适当的官能团（电子要求）创建扩展的缀合系统。 （b）发光期间附着的官能团和吖啶酮部分的共面性（几何要求）。 （c）所述官能团必须由至少一个芳环和一个供电子原子或基团组成，所述原子或基团具有额外的一对电子，所述电子可以容易地离域到扩展的pI系统中，所述杂原子直接连接或构建到其中， 与发光吖啶酮的吸电子羰基部分稳定的扩展共振。 这种以阴离子形式存在的供电子原子或基团对于进一步发射波长的红移是特别有效的。 组B：（a）在吖啶鎓核的C-2，C-4，C-5或C-7位置的一个或多个位置上直接连接电子给体原子或具有额外的一对或多对 电子。 如果使用多于一个供电子实体，则供电子实体可以相同或不同。 这种以阴离子形式存在的供电子原子或基团对于进一步发射波长的红移是特别有效的。 对于满足上述标准的分子如LEAE，期望并观察到3-HS-DMAE和超过500nm并且到达NIR区域的2-羟基-DMAE长波长发射。 优选地，与传统的吖啶鎓化合物相比，具有可比量子产率的NIR-AC的效用与使用具有良好到极好的检测效率的发光检测器结合在一起。 为了实现有效的NIR信号检测并促进诊断测定的执行，本发明的另一个目的是提出一种概念以及用现有技术的电荷耦合器件（CCD）检测器代替 在传统的全自动或半自动分析仪如马萨诸塞州Walpole的Chiron Diagnostics的MLA-II中使用红色不敏感的光电倍增管（PMT）。