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194 条记录 (耗时 0.017 秒)

    A LABELLING METHOD TO DISTINGUISH ISOBARIC AMINO ACIDS AND AMINO ACID COMBINATIONS
    5.
    发明申请
    A LABELLING METHOD TO DISTINGUISH ISOBARIC AMINO ACIDS AND AMINO ACID COMBINATIONS 审中-公开

    公开(公告)号：WO2020124252A1

    公开(公告)日：2020-06-25

    申请号：PCT/CA2019/051870

    申请日：2019-12-19

    申请人： RAPID NOVOR INC.

    发明人： LE BIHAN, Thierry ,  MA, Bin ,  MCDONALD, Zac ,  LIU, Qixin ,  TAYLOR, Paul

    IPC分类号： G01N33/483 ,  C12Q1/37 ,  G01N1/28 ,  C07K1/107 ,  C07K1/10

    摘要： A method for increasing peptide fragmentation by labelling the peptide at the C-terminal end with a guanidinium group or other basic functional group and distinguishing isobaric amino acids and amino acid combinations of asparagine and glycine-glycine; glutamine and glycine-alanine; and/or glutamine and alanine-glycine, during polypeptide sequencing. The method involves: obtaining a peptide of interest and/or digesting a polypeptide of interest with a protease, such as pepsin, chymotrypsin or trypsin, or by chemical cleavage to produce shorter peptides; reacting the obtained and/or generated peptides with a coupling reagent to derivatize the free C-terminal carboxylic acid function of the peptides, thus adding a basic functional group rendering C-terminal peptide fragment ions detectable by mass spectrometry; selecting a charge state of 2+ or more, and fragmenting the derivatized peptides in a mass spectrometer under conditions effective to generate at least w ions; and detecting the w ions by mass spectrometry, and identifying derivatized peptides which incorporate the additional mass of the basic functional group.

    PNA OLIGOMERS AND RELATED METHODS
    6.
    发明申请
    PNA OLIGOMERS AND RELATED METHODS 审中-公开

    公开(公告)号：WO2020124017A3

    公开(公告)日：2020-06-18

    申请号：PCT/US2019/066351

    申请日：2019-12-13

    申请人： TRUCODE GENE REPAIR, INC.

    发明人： SRIVASTAVA, Tumul

    IPC分类号： C07K1/06 ,  C07K1/16 ,  C07K1/10

    摘要： The present invention is directed to PNA oligomers that comprise an N-terminal bis- protected-diamine amino acid (e.g., a bis-Fmoc-protected lysine) moiety. The presence of the bis-protected diamine moiety can be used as a purification handle that permits separation between fully elongated PNA oligomer from truncated/capped failure sequences. After purification/separation, the protecting groups can then be removed from the PNA oligomer, and the resulting deprotected PNA oligomer optionally again purified. The methods disclosed herein are particularly useful for purifying longer PNA oligomers (e.g., oligomers of greater than 18 residues in length), including tail-clamp PNA oligomers, and/or PNA oligomers comprising two or more unnatural nucleobases.

    METHOD OF PREPARING TDM-621
    9.
    发明申请
    METHOD OF PREPARING TDM-621 审中-公开
    制备TDM-621的方法

    公开(公告)号：WO2017018835A1

    公开(公告)日：2017-02-02

    申请号：PCT/KR2016/008306

    申请日：2016-07-28

    申请人： DAEWOONG PHARMACEUTICAL CO., LTD.

    发明人： CHOI, Jong Yun ,  LEE, Yeon Gun ,  JANG, Woo Young ,  YOON, Hee Kyoon ,  KIM, Seung Woo

    IPC分类号： C07K1/02 ,  C07K1/06 ,  C07K1/10 ,  C07K7/08

    CPC分类号： C07K7/08

    摘要： Disclosed is a method of preparing TDM-621. According to the present invention, conventional problems of a solid-phase synthesis method can be addressed, and thus, high-purity and high-yield TDM-621 can be prepared in a relatively simple method. Therefore, a novel method of preparing TDM-621 according to the present invention is suitable and advantageous in commercially, massively producing TDM-621.

    摘要翻译： 公开了一种制备TDM-621的方法。 根据本发明，可以解决固相合成方法的常规问题，因此可以以相对简单的方法制备高纯度和高产率的TDM-621。 因此，根据本发明的制备TDM-621的新方法在商业上大规模生产TDM-621中是合适的和有利的。

    METHOD FOR CROSS-LINKING PEPTIDES
    10.
    发明申请
    METHOD FOR CROSS-LINKING PEPTIDES 审中-公开
    交联肽的方法

    公开(公告)号：WO2012085279A2

    公开(公告)日：2012-06-28

    申请号：PCT/EP2011/073974

    申请日：2011-12-23

    申请人： UNIVERSITEIT GENT ,  MADDER, Annemieke ,  HOOGEWIJS, Kurt ,  DECEUNINCK, Annelies ,  CARRETTE, Lieselot

    发明人： MADDER, Annemieke ,  HOOGEWIJS, Kurt ,  DECEUNINCK, Annelies ,  CARRETTE, Lieselot

    IPC分类号： C07K1/10 ,  C07K14/36

    CPC分类号： C07K1/113 ,  C07K1/006 ,  C07K1/1075 ,  C07K1/1077 ,  C07K1/13 ,  C12N9/0051 ,  C12Y108/01009

    摘要： The present invention relates to a method for cross-linking peptides using an activated furan-moiety. In particular, the present invention provides a method for cross-linking peptides comprising the steps of: a) providing a composition comprising furan-peptides, said furan-peptides comprising at least one amino acid comprising a furan-moiety; b) contacting said composition comprising furan-peptides with second peptides, thereby obtaining a mixture comprising furan-peptides and second peptides; c) adding an activation signal to said mixture of step b), thereby activating said furan-peptides to activated furan-peptides, and d) reacting said activated furan-peptides with said second peptides, thereby cross-linking said activated furan-peptides with said second peptides.

    摘要翻译： 本发明涉及使用活化的呋喃部分交联肽的方法。 具体而言，本发明提供了用于交联肽的方法，其包括以下步骤：a）提供包含呋喃肽的组合物，所述呋喃肽包含至少一个包含呋喃部分的氨基酸; b）使所述包含呋喃肽的组合物与第二肽接触，由此获得包含呋喃肽和第二肽的混合物; c）向步骤b）的所述混合物添加活化信号，从而将所述呋喃肽激活成活化的呋喃肽，和d）使所述活化的呋喃肽与所述第二肽反应，由此使所述活化的呋喃肽与 所述第二肽。