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公开(公告)号：WO2011017164A1
公开(公告)日：2011-02-10
申请号：PCT/US2010/043546
申请日：2010-07-28
发明人： FISCHER, Gerald W. , DAUM, Luke T.
IPC分类号： G01N33/48
CPC分类号： C12Q1/6851 , C12Q1/701
摘要： Disclosed are oligonucleotide amplification primers and detection probes specific for the amplification and detection of pathogenic organisms, including for example, specific Influenza A H1N1 viral isolates. Also disclosed is a biological organism identification kit including the disclosed nucleic acid probes and primers, as well as thermal cycling reagents that is both portable and durable, and may also be self-contained for remote, or in-field analysis and identification of particular influenza isolates from a variety of biological specimen types.
摘要翻译： 公开了对病原生物体的扩增和检测特异的寡核苷酸扩增引物和检测探针，包括例如特异性甲型H1N1流感病毒分离株。 还公开了包括所公开的核酸探针和引物以及便携式和耐用的热循环试剂的生物有机体鉴定试剂盒，并且也可以是独立的用于远程或现场分析和鉴定特定流感 从各种生物样本类型分离。
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公开(公告)号：WO2009085355A3
公开(公告)日：2009-07-09
申请号：PCT/US2008/078499
申请日：2008-10-01
IPC分类号： C12Q1/68
摘要： Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.
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3.发明申请COMPOSITIONS AND METHODS FOR DETECTING AND IDENTIFYING NUCLEIC ACID SEQUENCES IN BIOLOGICAL SAMPLES 审中-公开用于检测和鉴定生物样品中核酸序列的组合物和方法
公开(公告)号：WO2012149188A2
公开(公告)日：2012-11-01
申请号：PCT/US2012035253
申请日：2012-04-26
发明人： FISCHER GERALD W , DAUM LUKE T
CPC分类号： C12Q1/6806 , C12N15/1003 , C12Q1/689 , C12Q2600/16 , C12Q2600/166
摘要： The invention is directed to compositions and methods for isolating, detecting, amplifying, and quantitating pathogen-specific nucleic acids in a biological sample. The invention also provides diagnostic kits containing specific amplification primers, and labeled detection probes that specifically bind to the amplification products obtained therefrom. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more pathogens, including for example Influenza virus and Mycobacterium tuberculosis, from a wide variety of samples including those of biological, environmental, clinical and/or veterinary origin.
摘要翻译： 本发明涉及用于分离，检测，扩增和定量生物样品中病原体特异性核酸的组合物和方法。 本发明还提供了包含特异性扩增引物的诊断试剂盒，以及特异性结合从其获得的扩增产物的标记检测探针。 还公开了用于分离和表征对于一种或多种病原体（包括例如流感病毒和结核分枝杆菌）特异性的核酸的组合物和方法，所述多种样品包括生物，环境，临床和/或兽医 起源。
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公开(公告)号：WO2009029686A1
公开(公告)日：2009-03-05
申请号：PCT/US2008/074521
申请日：2008-08-27
发明人： FISCHER, Gerald W. , DAUM, Luke T.
IPC分类号： A61K39/295 , A61K39/145 , A61K39/085 , A61K39/116 , A61K39/102 , A61P31/16 , A61P31/04
CPC分类号： C07K14/005 , A61K39/085 , A61K39/092 , A61K39/102 , A61K39/12 , A61K39/145 , A61K2039/525 , A61K2039/5252 , A61K2039/5254 , A61K2039/543 , A61K2039/545 , A61K2039/55566 , A61K2039/575 , A61K2039/6037 , A61K2039/6043 , A61K2039/6068 , A61K2039/70 , C07K7/06 , C07K14/33 , C07K14/35 , C07K2319/40 , C07K2319/42 , C12N7/00 , C12N2760/16122 , C12N2760/16134 , C12N2760/16161
摘要： Disclosed are immunogenic compositions, and methods for their use in the formulation and administration of therapeutic and prophylactic pharmaceutical agents. In particular, the invention provides immunogenic compositions and methods for preventing, treating, and/or ameliorating microbial infection, including, for example, influenza, or one or more symptoms thereof.
摘要翻译： 公开了免疫原性组合物及其在配制和施用治疗和预防药剂中的用途。 特别地，本发明提供了用于预防，治疗和/或改善微生物感染的免疫原性组合物和方法，包括例如流感或其一种或多种症状。
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公开(公告)号：WO2015031787A3
公开(公告)日：2015-05-28
申请号：PCT/US2014053471
申请日：2014-08-29
发明人： FISCHER GERALD W , DAUM LUKE T , SEI CLARA JABET
CPC分类号： A61K39/04 , A61K39/12 , A61K39/40 , A61K2039/521 , A61K2039/55505 , A61K2039/55566 , A61K2039/575 , A61K2039/6037 , A61K2039/6081 , A61K2039/70 , C07K14/35 , C07K16/1289 , C07K2317/73 , C07K2319/00 , C07K2319/31 , C12N2760/16134 , G01N33/5055
摘要： The invention is directed to compositions and methods for generating or enhancing the immune system of a patient against infection by a pathogen, and in particular MTB. Compositions of the invention contain one or more non-naturally occurring antigens that generate an effective cellular or humoral immune response to MTB and/or antibodies that are specifically reactive to mycolic acid or to the surface of MTB. The greater activity of the immune system generated by a vaccine of the invention involve an conjugation of peptides to increase in the generation of memory T cells that provide for a greater and/or longer lived or extended response to an MTB infection. Preferably a response involves an increased generation of antibodies that enhance immunity against MTB infection and promote an enhanced phagocytic response.
摘要翻译： 本发明涉及用于产生或增强患者免受病原体感染，特别是MTB感染的免疫系统的组合物和方法。 本发明的组合物含有一种或多种非天然存在的抗原，其产生对MTB和/或抗体的有效细胞或体液免疫应答，所述MTB和/或抗体对霉酚酸或对MTB的表面具有特异性的反应性。 由本发明的疫苗产生的免疫系统的更大活性涉及肽的缀合，以增加记忆T细胞的产生，其提供对MTB感染的更大和/或更长寿命或延长的应答。 优选地，响应涉及提高针对MTB感染的免疫力的抗体的增加的产生并且促进增强的吞噬反应。
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公开(公告)号：WO2015100448A3
公开(公告)日：2015-10-29
申请号：PCT/US2014072506
申请日：2014-12-29
IPC分类号： A61K38/43 , A61K38/48 , C12N9/52 , G01N33/569
CPC分类号： A61K38/4886 , A61K9/0073 , A61K9/16 , A61K9/50 , A61K45/06 , A61L29/16 , A61L31/16 , A61L2300/254 , A61L2300/404 , A61L2300/45 , C12N9/6489 , C12Y304/24075
摘要： The present invention is directed to compositions and methods for preventing and/or treating diseases and disorders of patients caused by non-Staphylococcal microorganisms. In particular, compositions and methods contain lysostaphin, altered forms of lysostaphin as compared to wild-type, and synergistic combinations of lysostaphin plus additional conventional treatments such as other enzyme, antibiotic and/or antibody treatment. The invention is also directed to detecting and identifying altered forms of lysostaphin that possess increased efficacy against infections as compared to wild-type lysostaphin, and forms that generate a minimal or no immune response in a patient. The invention is also directed to method of manufacturing lysostaphin and altered forms of lysostaphin, and compositions that direct the lysostaphin to the site of the infection such as aerosolized nanoparticles.
摘要翻译： 本发明涉及用于预防和/或治疗由非葡萄球菌微生物引起的患者疾病和病症的组合物和方法。 特别地，组合物和方法含有溶葡萄球菌素，与野生型相比的溶葡萄球菌素的改变形式，以及溶葡萄球菌素加其他常规治疗如其它酶，抗生素和/或抗体治疗的协同组合。 本发明还涉及检测和鉴定与野生型溶葡萄球菌素相比具有增强的感染效力的溶葡萄球菌的改变形式，以及在患者体内产生最小或无免疫应答的形式。 本发明还涉及制备溶葡萄球菌素和改变形式的溶葡萄球菌素的方法，以及将溶葡萄球菌引导至感染部位如烟雾化纳米粒子的组合物。
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公开(公告)号：WO2008079463A2
公开(公告)日：2008-07-03
申请号：PCT/US2007/078025
申请日：2007-09-10
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/686 , C12Q1/701 , C12Q1/702
摘要： A biological organism identification product, and methods of using the same, that include a collection device to collect one or more sample organisms, a fixing and transporting composition present in an amount sufficient to kill the sample organism(s) associated with the collection device, an extraction member to extract a sufficient amount of genomic nucleic acid from the sample organism(s) to facilitate identification; and a polymerase chain reaction component into which the sufficient amount of genomic nucleic acid can be dissolved. The amplified genomic material is exposed to molecules that bind to predetermined genomic sequences, providing the identification feature of the product. The biological organism identification product may be portable, durable, and self-contained.
摘要翻译： 一种生物有机体鉴定产物及其使用方法，其包括收集装置以收集一种或多种样品生物体，以足以杀死与收集装置相关联的样品生物体的量存在的固定和运输组合物， 提取成员从样品生物体中提取足量的基因组核酸以促进鉴定; 以及能够溶解足够量的基因组核酸的聚合酶链式反应组分。 将扩增的基因组材料暴露于结合预定基因组序列的分子，提供产物的鉴定特征。 生物体识别产品可以是便携式的，耐用的和独立的。
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公开(公告)号：WO2010123908A1
公开(公告)日：2010-10-28
申请号：PCT/US2010/031761
申请日：2010-04-20
发明人： FISCHER, Gerald W. , DAUM, Luke T.
CPC分类号： C12Q1/6876 , C12N15/1003 , C12Q1/6806 , C12Q1/6848 , C12Q1/6851 , C12Q2600/166 , C12Q2523/113 , C12Q2527/125 , C12Q2531/113 , C12Q2545/101 , C12Q2527/127
摘要： Disclosed are compositions for collecting, storing, and transporting populations of nucleic acids from biological specimens, and clinical, forensic, or environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. In particular embodiments, the invention provides a single, one-step, sample collection/transport/storage formulation containing a known quantity of a non-genomic, nucleic acid carrier molecule that serves as an internal reference control to monitor the fidelity of the collection/transportation medium, and measure the integrity of nucleic acids subsequently isolated and purified from the processed sample.
摘要翻译： 公开了用于收集，储存和运输来自生物标本，临床，法医或环境样品的核酸群体的组合物。 还公开了将这些组合物用作用于杀死病原体，灭活核酸酶以及从样品中的其他细胞组分释放多核苷酸的一步制剂以及在进一步加工或测定之前使核酸稳定的方法。 在具体实施方案中，本发明提供了含有已知量的非基因组核酸载体分子的单一一步样品收集/转运/储存制剂，其用作内部参考对照以监测收集/转运/ 运输介质，并测量随后从处理的样品中分离和纯化的核酸的完整性。
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