公开(公告)号：WO2008016988A9

公开(公告)日：2008-02-07

申请号：PCT/US2007/074990

申请日：2007-08-01

Applicant: BECKER, Michael M. ,  MAJLESSI, Mehrdad R. ,  GEN-PROBE INCORPORATED

Inventor： BECKER, Michael M. ,  MAJLESSI, Mehrdad R.

IPC: C12Q1/68 ,  C07H21/04

Abstract: Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed.

公开(公告)号：WO2006121888A2

公开(公告)日：2006-11-16

申请号：PCT/US2006017472

申请日：2006-05-05

Applicant: GEN PROBE INC ,  POLLNER REINHOLD B ,  BECKER MICHAEL M ,  MAJLESSI MEHRDAD R

Inventor： POLLNER REINHOLD B ,  BECKER MICHAEL M ,  MAJLESSI MEHRDAD R

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  C12Q1/6834 ,  Y10T436/143333 ,  C12Q2537/125 ,  C12Q2523/113 ,  C12Q2565/519 ,  C12Q2527/101

Abstract: Methods for efficiently capturing a target nucleic acid from a sample by using a mixture that contains a capture probe specific for the target nucleic acid, the target nucleic acid, and a denaturant chemical, which mixture is incubated at elevated temperature for a short time, are disclosed. Compositions that include a capture probe that specifically binds to a target nucleic acid and a denaturant chemical, which when mixed with the target nucleic acid and incubated at elevated temperature for a short time, promote efficient hybridization of the capture probe and target nucleic acid are disclosed.

Abstract translation: 通过使用含有目标核酸特异性的捕获探针，目标核酸和变性剂化合物的混合物在高温下短时间培养来有效地从样品中捕获靶核酸的方法是： 披露。 公开了包含与目标核酸和变性剂化学物质特异性结合的捕获探针的组合物，当与目标核酸混合并在高温下温育短时间时，促进了捕获探针和靶核酸的有效杂交 。

公开(公告)号：WO2008016988A1

公开(公告)日：2008-02-07

申请号：PCT/US2007074990

申请日：2007-08-01

Applicant: GEN PROBE INC ,  BECKER MICHAEL M ,  MAJLESSI MEHRDAD R

Inventor： BECKER MICHAEL M ,  MAJLESSI MEHRDAD R

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12N15/1006 ,  C12N15/11 ,  C12N2310/18 ,  C12N2310/31 ,  C12N2310/321 ,  C12N2310/351 ,  C12N2330/30 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/70 ,  C12Q2565/519 ,  C12Q2565/518

Abstract: Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed.

Abstract translation: 通过使用捕获探针非特异性结合靶核酸并通过在捕获探针上具有一个成员的特异性结合对与固定化探针上的一个成员特异性结合固定的探针来从样品捕获靶核酸的方法 被披露。 公开了包含捕获探针的组合物，其通过结合反应混合物的溶液相中的特异性结合对的成员非特异性地结合靶核酸而特异性结合固定的探针。

公开(公告)号：WO2006121888A3

公开(公告)日：2006-11-16

申请号：PCT/US2006/017472

申请日：2006-05-05

Applicant: GEN-PROBE INCORPORATED ,  POLLNER, Reinhold B. ,  BECKER, Michael M. ,  MAJLESSI, Mehrdad R.

Inventor： POLLNER, Reinhold B. ,  BECKER, Michael M. ,  MAJLESSI, Mehrdad R.

IPC: C12Q1/68

Abstract: Methods for efficiently capturing a target nucleic acid from a sample by using a mixture that contains a capture probe specific for the target nucleic acid, the target nucleic acid, and a denaturant chemical, which mixture is incubated at elevated temperature for a short time, are disclosed. Compositions that include a capture probe that specifically binds to a target nucleic acid and a denaturant chemical, which when mixed with the target nucleic acid and incubated at elevated temperature for a short time, promote efficient hybridization of the capture probe and target nucleic acid are disclosed.