公开(公告)号：WO2011032040A1

公开(公告)日：2011-03-17

申请号：PCT/US2010/048526

申请日：2010-09-10

Applicant: CENTRILLION TECHNOLOGY HOLDING CORPORATION ,  ZHOU, Wei ,  MEI, Rui ,  MATSUZAKI, Hajime

Inventor： ZHOU, Wei ,  MEI, Rui ,  MATSUZAKI, Hajime

IPC: C12Q1/68

CPC classification number: C12Q1/6827

Abstract: The present invention provides methods for targeted sequencing of polynucleotide. In one aspect, the present invention provides a method of sequencing a target polynucleotide with fewer probes. In another aspect, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/ genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.

Abstract translation: 本发明提供了多核苷酸的靶向测序方法。 一方面，本发明提供了用较少探针测序靶多核苷酸的方法。 另一方面，本发明提供了用更长读数测序靶多核苷酸的方法。 本发明还提供了特异性，连接辅助测序/基因分型方法和连接捕获测序方法。 本发明的方法允许核酸的低成本，高通量和精确的测序。

公开(公告)号：WO2003010328A3

公开(公告)日：2003-02-06

申请号：PCT/US2002/023570

申请日：2002-07-23

Applicant: AFFYMETRIX, INC. ,  SU, Xing ,  MATSUZAKI, Hajime ,  KENNEDY, Giulia

Inventor： SU, Xing ,  MATSUZAKI, Hajime ,  KENNEDY, Giulia

IPC: C12Q1/68

Abstract: The presently claimed invention provides for novel methods and kits for reducing the complexity of a nucleic acid sample by providing non-gel based methods for size fractionation. In a preferred embodiment, size fractionation can be accomplished by varying conditions or reagents of a PCR reaction to amplify fragments of specific size ranges. The invention further provides for analysis of the above sample by hybridization to an array, which may be specifically designed to interrogate the desired fragments for particular characteristics, such as, for example, the presence or absence of a polymorphism.

公开(公告)号：WO2003010328A2

公开(公告)日：2003-02-06

申请号：PCT/US2002/023570

申请日：2002-07-23

Applicant: AFFYMETRIX, INC. ,  SU, Xing ,  MATSUZAKI, Hajime ,  KENNEDY, Giulia

Inventor： SU, Xing ,  MATSUZAKI, Hajime ,  KENNEDY, Giulia

IPC: C12Q

CPC classification number: C12Q1/6855 ,  C12Q2527/143 ,  C12Q2527/113

Abstract: The presently claimed invention provides for novel methods and kits for reducing the complexity of a nucleic acid sample by providing non-gel based methods for size fractionation. In a preferred embodiment, size fractionation can be accomplished by varying conditions or reagents of a PCR reaction to amplify fragments of specific size ranges. The invention further provides for analysis of the above sample by hybridization to an array, which may be specifically designed to interrogate the desired fragments for particular characteristics, such as, for example, the presence or absence of a polymorphism.

Abstract translation: 目前要求保护的发明提供了用于通过提供用于尺寸分级分离的非基于凝胶的方法来降低核酸样品的复杂性的新颖方法和试剂盒。 在优选的实施方案中，大小分级可以通过改变PCR反应的条件或试剂来扩增特定大小范围的片段来实现。 本发明进一步提供了通过与阵列的杂交分析上述样品，所述阵列可被特别设计为询问特定特征的期望片段，例如多态性的存在或不存在。

公开(公告)号：WO1997027317A1

公开(公告)日：1997-07-31

申请号：PCT/US1997001603

申请日：1997-01-22

Applicant: AFFYMETRIX, INC. ,  LOCKHART, David, J. ,  CHEE, Mark ,  GUNDERSON, Kevin ,  LAI, Chaoqiang ,  WODICKA, Lisa ,  CRONIN, Maureen, T. ,  LEE, Danny ,  TRAN, Huu, M. ,  MATSUZAKI, Hajime ,  McGALL, Glenn, H. ,  BARONE, Anthony, D.

Inventor： AFFYMETRIX, INC.

IPC: C12Q01/00

CPC classification number: C12Q1/6809 ,  C07H19/052 ,  C07H19/12 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q2600/156 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2521/501

Abstract: The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater then 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.

Abstract translation: 本发明提供用于鉴定两个或多个样品之间的核酸丰度差异（例如，表达水平）的简化方法。 该方法包括提供包含大量（例如大于1,000个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置是已知的。 来自两个或更多个样品的核酸样品（例如mRNA）与探针阵列杂交，并检测杂交模式。 样本之间的杂交模式的差异表明这些样品之间各种基因的表达差异。 本发明还提供了一种终止标记核酸的方法。 在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶连接到核酸上。 因此，例如，当核酸是RNA时，可以使用RNA连接酶连接标记的寡核糖核苷酸。 在另一个实施方案中，末端标记可以通过提供核酸，提供标记的核苷三磷酸和使用末端转移酶将核苷三磷酸与核酸连接来实现。