公开(公告)号：WO2009120731A2

公开(公告)日：2009-10-01

申请号：PCT/US2009/038164

申请日：2009-03-25

Applicant: E. I. DU PONT DE NEMOURS AND COMPANY ,  ALLIANCE FOR SUSTAINABLE ENERGY, LLC ,  VIITANEN, Paul, V. ,  TAO, Luan ,  ZHANG, Yuying ,  CAIMI, Perry, G. ,  MCCUTCHEN, Carol ,  MCCOLE, Laura ,  ZHANG, Min ,  CHOU, Yat-Chen ,  FRANDEN, Mary Ann

Inventor： VIITANEN, Paul, V. ,  TAO, Luan ,  ZHANG, Yuying ,  CAIMI, Perry, G. ,  MCCUTCHEN, Carol ,  MCCOLE, Laura ,  ZHANG, Min ,  CHOU, Yat-Chen ,  FRANDEN, Mary Ann

IPC: C12N1/20 ,  C12N9/92 ,  C12N15/52

CPC classification number: C12N9/92 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/1205 ,  C12P7/065 ,  Y02E50/17

Abstract: Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.

Abstract translation: 通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的突变启动子的嵌合木糖异构酶基因工程化发酵单胞菌菌株。 启动子指导木糖异构酶的增加表达，并且当将该菌株另外设计用于表达木酮糖激酶，转醛醇酶和转酮酶时，获得了木糖的改善利用。

公开(公告)号：WO2012006061A1

公开(公告)日：2012-01-12

申请号：PCT/US2011/042122

申请日：2011-06-28

Applicant: E. I. DU PONT DE NEMOURS AND COMPANY ,  CAIMI, Perry, G. ,  MCCOLE, Laura ,  TAO, Luan ,  TOMB, Jean-Francois ,  VIITANEN, Paul, V.

Inventor： CAIMI, Perry, G. ,  MCCOLE, Laura ,  TAO, Luan ,  TOMB, Jean-Francois ,  VIITANEN, Paul, V.

IPC: C12N9/90 ,  C12N1/21 ,  C12P7/06 ,  C12N1/22

CPC classification number: C12N9/90 ,  C12N1/20 ,  C12N9/92 ,  C12P7/065 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: Xylose-utilizing Zymomonas strains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.

Abstract translation: 发现木糖利用的发酵单胞菌菌株在含木糖培养基中生长时积累核酮糖。 工程化这些菌株以增加核糖-5-磷酸异构酶活性，导致核酮糖积累减少，生长改善，木糖利用率提高，乙醇生产增加。

公开(公告)号：WO2009120730A3

公开(公告)日：2009-12-10

申请号：PCT/US2009038158

申请日：2009-03-25

Applicant: DU PONT ,  ALLIANCE SUSTAINABLE ENERGY ,  VIITANEN PAUL V ,  TAO LUAN ,  ZHANG YUYING ,  CAIMI PERRY G ,  MCCUTCHEN CAROL ,  MCCOLE LAURA ,  ZHANG MIN ,  CHOU YAT-CHEN ,  FRANDEN MARY ANN

Inventor： VIITANEN PAUL V ,  TAO LUAN ,  ZHANG YUYING ,  CAIMI PERRY G ,  MCCUTCHEN CAROL ,  MCCOLE LAURA ,  ZHANG MIN ,  CHOU YAT-CHEN ,  FRANDEN MARY ANN

IPC: C12N15/09

CPC classification number: C12N15/74

Abstract: Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.

Abstract translation: 鉴定为运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的启动子的突变体，其直接改善可操作地连接的异源核酸的表达水平。 这些是用于在发酵单胞菌，酵母属和其他相关细菌中表达嵌合基因的高表达启动子。

公开(公告)号：WO2009120731A3

公开(公告)日：2009-11-12

申请号：PCT/US2009038164

申请日：2009-03-25

Applicant: DU PONT ,  ALLIANCE SUSTAINABLE ENERGY ,  VIITANEN PAUL V ,  TAO LUAN ,  ZHANG YUYING ,  CAIMI PERRY G ,  MCCUTCHEN CAROL ,  MCCOLE LAURA ,  ZHANG MIN ,  CHOU YAT-CHEN ,  FRANDEN MARY ANN

Inventor： VIITANEN PAUL V ,  TAO LUAN ,  ZHANG YUYING ,  CAIMI PERRY G ,  MCCUTCHEN CAROL ,  MCCOLE LAURA ,  ZHANG MIN ,  CHOU YAT-CHEN ,  FRANDEN MARY ANN

IPC: C12N1/20 ,  C12N9/92 ,  C12N15/52

CPC classification number: C12N9/92 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/1205 ,  C12P7/065 ,  Y02E50/17

Abstract: Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.

Abstract translation: 通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因突变启动子的嵌合木糖异构酶基因来改造发酵单胞菌菌株。 该启动子指导木糖异构酶表达的增加，并且当该菌株另外工程化用于表达木酮糖激酶，转醛醇酶和转酮醇酶时，获得改进的木糖利用。

公开(公告)号：WO2009120730A2

公开(公告)日：2009-10-01

申请号：PCT/US2009/038158

申请日：2009-03-25

Applicant: E. I. DU PONT DE NEMOURS AND COMPANY ,  ALLIANCE FOR SUSTAINABLE ENERGY, LLC ,  VIITANEN, Paul, V. ,  TAO, Luan ,  ZHANG, Yuying ,  CAIMI, Perry, G. ,  MCCUTCHEN, Carol ,  MCCOLE, Laura ,  ZHANG, Min ,  CHOU, Yat-chen ,  FRANDEN, Mary, Ann

Inventor： VIITANEN, Paul, V. ,  TAO, Luan ,  ZHANG, Yuying ,  CAIMI, Perry, G. ,  MCCUTCHEN, Carol ,  MCCOLE, Laura ,  ZHANG, Min ,  CHOU, Yat-chen ,  FRANDEN, Mary, Ann

IPC: C12N15/09

CPC classification number: C12N15/74

Abstract: Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas , Zymobacter , and other related bacteria.

Abstract translation: 鉴定了Z启动子的突变体。 运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因，其指导可操作连接的异源核酸的表达水平的改善。 这些是用于在发酵单胞菌属，发酵菌属和其它相关细菌中表达嵌合基因的高表达启动子。