公开(公告)号：WO2009040555A3

公开(公告)日：2009-09-24

申请号：PCT/GB2008003294

申请日：2008-09-29

Applicant: CELLAURA TECHNOLOGIES LTD ,  HILL STEPHEN JOHN ,  KELLAM BARRIE ,  MIDDELTON RICHARD JOHN

Inventor： HILL STEPHEN JOHN ,  KELLAM BARRIE ,  MIDDELTON RICHARD JOHN

IPC: C12N15/10

CPC classification number: C12N15/1075 ,  C07F5/022

Abstract: A method for generating a recombinant clonal cell line expressing a target cell surface receptor at a specific level of expression from a cell population comprising cells transfected with a plasmid encoding the cDNA sequence of the target receptor and expressing the target cell surface receptor, the method comprising (c) incubating the cell population with a receptor specific fluorescent ligand (d) selecting single cells from step (c) expressing the target cell surface receptor by monitoring the specific binding of the fluorescent ligand using flow cytometry; and novel fluorescent ligands.

Abstract translation: 产生表达来自细胞群的特定表达受体的靶细胞表面受体的重组克隆细胞系的方法，所述细胞群包含用编码靶受体的cDNA序列并表达靶细胞表面受体的质粒转染的细胞，所述方法包括 （c）用受体特异性荧光配体孵育细胞群;（d）通过使用流式细胞术监测荧光配体的特异性结合，从步骤（c）中选择表达靶细胞表面受体的单个细胞; 和新颖的荧光配体。

公开(公告)号：WO2009040555A2

公开(公告)日：2009-04-02

申请号：PCT/GB2008/003294

申请日：2008-09-29

Applicant: CELLAURA TECHNOLOGIES LTD ,  HILL, Stephen, John ,  KELLAM, Barrie ,  MIDDELTON, Richard, John

Inventor： HILL, Stephen, John ,  KELLAM, Barrie ,  MIDDELTON, Richard, John

IPC: C12N5/00 ,  C12N15/10

CPC classification number: C12N15/1075 ,  C07F5/022

Abstract: A method for generating a recombinant clonal cell line expressing a target cell surface receptor at a specific level of expression from a cell population comprising cells transfected with a plasmid encoding the cDNA sequence of the target receptor and expressing the target cell surface receptor, the method comprising (c) incubating the cell population with a receptor specific fluorescent ligand (d) selecting single cells from step (c) expressing the target cell surface receptor by monitoring the specific binding of the fluorescent ligand using flow cytometry; and novel fluorescent ligands.

Abstract translation: 一种生成表达靶细胞表面受体的重组克隆细胞系的方法，所述重组克隆细胞系来自包含用编码靶受体的cDNA序列的质粒转染并表达靶细胞表面受体的细胞的细胞群，所述方法包括 （c）用受体特异性荧光配体孵育细胞群体（d）通过使用流式细胞术监测荧光配体的特异性结合来选择表达靶细胞表面受体的步骤（c）中的单个细胞; 和新型荧光配体。