公开(公告)号：WO2013106007A1

公开(公告)日：2013-07-18

申请号：PCT/US2012/029587

申请日：2012-03-18

Applicant: PATHWAY GENOMICS ,  BECKER, David ,  PLANTE, James ,  MORENO, Tanya ,  WANG, Cindy

Inventor： BECKER, David ,  PLANTE, James ,  MORENO, Tanya ,  WANG, Cindy

IPC: C12N15/10 ,  B01L3/14 ,  C12M3/08

CPC classification number: B01L3/508 ,  A61B10/0051 ,  A61B10/0096 ,  A61B2010/0006 ,  B01L3/502 ,  B01L3/50825 ,  B01L99/00 ,  B01L2200/185 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/06 ,  B01L2300/0672 ,  C12N15/1006 ,  C12Q1/6806

Abstract: Biological sample collection kits are devised with physical features to enable a high performance collection system which delivers preprocessed biological matter via conventional shipping means to a testing laboratories. In particular, users deposit biological matter such as saliva or blood into a receiving vessel. By sealing the container, the user causes release of a premixed solution containing preservatives and optionally lysis reagents. In addition, a purification agent is arranged to bind to target molecules and facilitate their removal from solution. These time consuming processes occur while the sample is in transit to the testing facility such that when it arrives, it is in a preconditioned state immediately ready for execution of washing steps. Thus, the high performance containers taught herein are useful for collection biological samples and performing initial process steps on received matter - steps which are largely effected during the shipping stage of the transfer process.

Abstract translation: 生物样本收集套件设计有物理特征，以实现高性能收集系统，其通过常规运输方式将预处理的生物物质提供给测试实验室。 特别地，用户将生物物质如唾液或血液沉积到接收容器中。 通过密封容器，使用者引起含有防腐剂和任选裂解试剂的预混溶液的释放。 此外，将净化剂配置成与靶分子结合并促进它们从溶液中的去除。 这些耗时的过程发生在样品转运到测试设备时，使得当其到达时，它处于预处理状态，立即准备好执行洗涤步骤。 因此，本文中教导的高性能容器对于收集生物样品是有用的，并且对接收物质进行初步的处理步骤 - 在转移过程的运输阶段中主要影响的步骤。

公开(公告)号：WO2013141835A1

公开(公告)日：2013-09-26

申请号：PCT/US2012/029586

申请日：2012-03-18

Applicant: PATHWAY GENOMICS ,  MORENO, Tanya ,  WANG, Cindy ,  BECKER, David

Inventor： MORENO, Tanya ,  WANG, Cindy ,  BECKER, David

IPC: C12M1/00 ,  G01N21/00

CPC classification number: B01L3/50851 ,  B01L7/52 ,  B01L2300/0636

Abstract: Dual mode genetics testing systems are devised about a single element testing platform. A microfluidic network and system of interconnected receiving cells and reaction vessels supports at the same time genotyping and copy number analysis where the platform may be subject to a common thermal cycle schedule to cause the proper reactions (DNA replication) necessary in both test types. Further, the microfluidic platform which includes reaction vessels for genotyping which are spatially removed from reaction vessels for copy number analysis, is coupled to optical scanner and detection systems specifically arranged to apply test specific detection routines on each of these distinct regions or portions of the dual mode test platform.

Abstract translation: 关于单个元素测试平台设计了双模式遗传学测试系统。 互连接收细胞和反应容器的微流体网络和系统同时支持基因分型和拷贝数分析，其中平台可能经受常见的热循环进程，以引起两种测试类型中必需的适当反应（DNA复制）。 此外，包括用于基因分型的反应容器的微流体平台被从用于拷贝数分析的反应容器中空间移除，被耦合到光学扫描器和检测系统，该系统被特别设置为在这些不同区域或双重部分的每一个上应用测试特异性检测程序 模式测试平台。