公开(公告)号：WO2016181228A2

公开(公告)日：2016-11-17

申请号：PCT/IB2016/000933

申请日：2016-05-09

Applicant: MYBIOTIX PHARMA LTD.

Inventor： BARAM, David ,  DIAMANT, Rachel ,  DABOUSH, David

IPC: C12N1/20

CPC classification number: C12N1/20 ,  A61K35/74 ,  A61K35/747 ,  A61K2035/11 ,  C12N11/02 ,  C12N11/04 ,  C12N11/12 ,  C12N11/14

Abstract: The present invention provides a method, wherein the method forms a biofilm, wherein the biofilm comprises a population of at least one bacterial strain attached to particles, wherein the biofilm is configured to colonize a gut of a subject in need thereof for at least five days, when ingested by the subject, the method comprising: a. obtaining a population comprising at least one strain of bacteria; b. inoculating a growth medium containing particles with the population comprising at least one strain of bacteria; c. incubating the particles with the population comprising at least one bacterial strain for a time sufficient for the population of at least one strain of bacteria to attach to the particles; and d. culturing the population comprising at least one strain of bacteria attached to the particles in a growth medium, for a time sufficient to form a biofilm.

Abstract translation: 本发明提供了一种方法，其中所述方法形成生物膜，其中所述生物膜包含连接至颗粒的至少一种细菌菌株的群体，其中所述生物膜被配置为将有需要的受试者的肠定殖至少五天 当被检体摄入时，所述方法包括：a。 获得包含至少一种细菌菌株的群体; 湾 接种含有至少一种细菌菌株的群体的含有颗粒的生长培养基; C。 将所述颗粒与包含至少一种细菌菌株的群体一起孵育足以使至少一种细菌菌群附着于颗粒的时间; 和d。 培养包含在生长培养基中附着到颗粒上的至少一种细菌菌株至少足以形成生物膜的时间。

公开(公告)号：WO2016181228A3

公开(公告)日：2017-01-05

申请号：PCT/IB2016000933

申请日：2016-05-09

Applicant: MYBIOTIX PHARMA LTD

Inventor： BARAM DAVID ,  DIAMANT RACHEL ,  DABOUSH DAVID

IPC: A23L33/125 ,  C12N11/04

CPC classification number: C12N1/20 ,  A61K35/74 ,  A61K35/747 ,  A61K2035/11 ,  C12N11/02 ,  C12N11/04 ,  C12N11/12 ,  C12N11/14

Abstract: The present invention provides a method, wherein the method forms a biofilm, wherein the biofilm comprises a population of at least one bacterial strain attached to particles, wherein the biofilm is configured to colonize a gut of a subject in need thereof for at least five days, when ingested by the subject, the method comprising: a. obtaining a population comprising at least one strain of bacteria; b. inoculating a growth medium containing particles with the population comprising at least one strain of bacteria; c. incubating the particles with the population comprising at least one bacterial strain for a time sufficient for the population of at least one strain of bacteria to attach to the particles; and d. culturing the population comprising at least one strain of bacteria attached to the particles in a growth medium, for a time sufficient to form a biofilm.

Abstract translation: 本发明提供了一种方法，其中所述方法形成生物膜，其中所述生物膜包含连接至颗粒的至少一种细菌菌株的群体，其中所述生物膜被配置为将有需要的受试者的肠定殖至少五天 当被检体摄入时，所述方法包括：a。 获得包含至少一种细菌菌株的群体; 湾 接种含有至少一种细菌菌株的群体的含有颗粒的生长培养基; C。 将所述颗粒与包含至少一种细菌菌株的群体一起孵育足以使至少一种细菌菌群附着于颗粒的时间; 和d。 培养包含在生长培养基中附着到颗粒上的至少一种细菌菌株至少足以形成生物膜的时间。