公开(公告)号：WO2009034563A2

公开(公告)日：2009-03-19

申请号：PCT/IE2008/000087

申请日：2008-09-15

Applicant: NANOCOMMS PATENTS LIMITED ,  REDMOND, Gareth ,  KEWELL, Adrian ,  KRUGER, Jan ,  VON PAPEN, Georg

Inventor： REDMOND, Gareth ,  KEWELL, Adrian ,  KRUGER, Jan ,  VON PAPEN, Georg

IPC: B01L3/00 ,  G01N21/05 ,  G01N33/543

CPC classification number: G01N21/05 ,  B01L3/5023 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/5029 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2200/16 ,  B01L2300/043 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0406 ,  G01N33/54386 ,  G01N2021/0346 ,  G01N2021/054 ,  G01N2021/058

Abstract: An analysis system comprises a sampling cartridge (11) comprising a housing (21, 22) having an inlet (23) for receiving a fluid sample, a sensor (26, 45), and a guide (40, 42) extending between the inlet and the sensor for guiding sample into contact with the sensor. The system also has an optical detection reader (13) for optically inspecting the sensor. The cartridge housing has an inspection window (34) and the reader (13) comprises a socket to receive the cartridge (11), and an optical system (1183, 1186) for inspecting the sensor through the window. The cartridge comprises parallel microfluidic channel (42) for flow of sample from the inlet into contact with the sensor. The sensor comprises discrete sensor pads (45) in at least one channel, with an antibody, an antigen, or molecular imprinted polymer. The channels are of microfluidic size, having a cross-sectional area in the range of about 0.3 mm 2 to about 5 mm 2 . At least one channel comprises a reagent pad (43) upstream of the sensor (45). The inlet of ihe cartridge comprises an extraction chamber (23) communicating with a draining chamber having a top reservoir (61) and a bottom reservoir (63), in turn communicating with a distribution chamber (40). Together, these chambers and the interfaces between them guide sample flow in a uniform manner between the channels and also remove impurity particles and bubbles.

Abstract translation: 分析系统包括采样盒（11），该采样盒包括具有用于接收流体样本的入口（23）的壳体（21,22），传感器（26,45）和引导件 在入口和传感器之间延伸，用于引导样本与传感器接触。 该系统还具有用于光学检测传感器的光学检测读取器（13）。 盒壳体具有检查窗口（34），读取器（13）包括用于接收盒（11）的插口以及用于通过窗口检查传感器的光学系统（1183,1186）。 盒包括平行的微流体通道（42），用于使样品从入口流动到与传感器接触。 该传感器包括至少一个通道中的离散传感器垫（45），与抗体，抗原或分子印记聚合物。 通道具有微流体尺寸，其横截面积在约0.3mm 2至约5mm 2的范围内。 至少一个通道包括传感器（45）上游的试剂垫（43）。 盒的入口包括与具有顶部储存器（61）和底部储存器（63）的排放室连通的提取室（23），所述排出室又与分配室（40）连通。 这些室和它们之间的界面一起在通道之间均匀地引导样品流动，并且还去除杂质颗粒和气泡。

公开(公告)号：WO2005025748A1

公开(公告)日：2005-03-24

申请号：PCT/IE2004/000126

申请日：2004-09-17

Applicant: NANOCOMMS PATENTS LIMITED ,  O'BRIEN, Peter ,  KRÜGER, Jan ,  REDMOND, Gareth

Inventor： O'BRIEN, Peter ,  KRÜGER, Jan ,  REDMOND, Gareth

IPC: B01L3/00

CPC classification number: B01L3/502707 ,  B01L2200/12 ,  G02B6/138 ,  G02B6/30 ,  G02B6/423

Abstract: An embossing master (20) is produced by successively applying epoxy layers (2, 10) over a silicon substrate (1) and selectively exposing them to UV to cross-link according to a pattern. Non-exposed epoxy is developed away to leave a pattern of cured epoxy at each level. This provides a multi-level master, with a desired 3D configuration. The master (20) is then used to emboss a polymer blank to provide a substrate (80) and a different master is used to emboss a blank to provide a superstrate (90). The substrate (80) has aligned socket and channel grooves (80, 81) and the superstrate (90) has a socket groove (91). When the superstrate is mated with the substrate, there is a socket for receiving a fluidic capillary or a detection waveguide. The capillary or waveguide is aligned with the channel for optimum fluidic flow or optical detection.

Abstract translation: 通过在硅衬底（1）上依次施加环氧树脂层（2,10）并选择性地将它们暴露于UV以根据图案交联来制造压花母版（20）。 不暴露的环氧树脂被开发出来，以便在每个层面留下固化的环氧树脂的图案。 这提供了一个具有所需3D配置的多级主控。 然后使用母版（20）压印聚合物毛坯以提供基底（80），并且使用不同的母版来压印毛坯以提供覆层（90）。 衬底（80）具有对准的插座和沟槽（80,81），并且覆盖层（90）具有插槽（91）。 当衬底与衬底配合时，存在用于接收流体毛细管或检测波导的插座。 毛细管或波导与通道对准以获得最佳流体流动或光学检测。