公开(公告)号：WO2019116033A1

公开(公告)日：2019-06-20

申请号：PCT/GB2018/053609

申请日：2018-12-12

Applicant: OXFORD UNIVERSITY INNOVATION LIMITED

Inventor： WALSH, Edmond ,  COOK, Peter Richard ,  SOITU, Cristian

IPC: B01L3/00

CPC classification number: B01L3/5088 ,  B01L3/502707 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/141 ,  B01L2200/142 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2300/161

Abstract: Methods and apparatus for manufacturing a microfluidic arrangement are disclosed. In one arrangement, a selected region of a substrate surface is modified by moving a probe over the selected region while maintaining contact between the probe and the substrate surface. The modification is such that an equilibrium contact angle of the first liquid is higher than without the modification. The modified selected region forms one or more isolated regions on the substrate surface by surrounding each isolated region. The modified selected region forming a closed path around each isolated region. A microfluidic arrangement is formed that comprises a body of the first liquid in direct contact with each isolated region, and a second liquid in direct contact with, and covering, each body of first liquid and the modified selected region, wherein the second liquid is immiscible with the first liquid, and each body of first liquid is held in shape predominantly by surface tension and substantially fills each isolated region.

公开(公告)号：WO2018200916A1

公开(公告)日：2018-11-01

申请号：PCT/US2018/029726

申请日：2018-04-27

Applicant: CORNING INCORPORATED

Inventor： BOEK, Heather, Debra ,  DANNOUX, Thierry, Luc, Alain

IPC: B01L3/00 ,  C03B23/03

CPC classification number: C03B23/0302 ,  B01L3/00 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/12 ,  C03B17/02 ,  C03B17/064 ,  C03B23/02 ,  C03B23/03 ,  C03B23/0357 ,  C03C2204/08

Abstract: A multi-well glass-containing structure, and system and method to manufacture the structure are provided. The structure can be a glass plate having a well defined by a rim at a top of the plate to define a well opening, a well bottom at a bottom of the plate spaced away from the rim by a well wall extending from the rim to the well bottom. A well aspect ratio of the depth of the well to a maximum surface dimension of the well opening can be in a range from 40% to 100%. The inner surface of the well can have an average roughness, Ra, of less than 600 nm. The system can include a mold with a coefficient of thermal expansion that matches the glass-containing structure and the method can include forming the glass plate at a viscosity of about 10 5 to 10 7.6 poises.

公开(公告)号：WO2018039338A1

公开(公告)日：2018-03-01

申请号：PCT/US2017/048202

申请日：2017-08-23

Applicant: 10X GENOMICS, INC.

Inventor： BHARADWAJ, Rajiv ,  WHEELER, Tobias, Daniel ,  NESS, Kevin ,  HINDSON, Benjamin

IPC: B01F3/08 ,  C12Q1/68 ,  B01J2/06

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F3/0811 ,  B01F13/0086 ,  B01F2215/0037 ,  B01J2/06 ,  B01L2200/0673 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/16 ,  C12Q1/6844 ,  C12Q2531/113 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

Abstract translation: 提供了一种微流体乳液微滴生成系统及其使用方法。 该系统可以包括微流体基底，该微流体基底具有被配置和布置用于产生乳液液滴的流动路径，流动路径中的至少一个纹理化表面被配置和布置用于诱导乳液液滴的表面介导的聚结; 并且在流路中至少有一个通道连接处形成乳滴。

公开(公告)号：WO2017160909A1

公开(公告)日：2017-09-21

申请号：PCT/US2017/022411

申请日：2017-03-15

Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA ,  RENSSELAER POLYTECHNIC INSTITUTE

Inventor： DRNDIC, Marija ,  MASIH DAS, Paul C. ,  MEUNIER, Vincent

IPC: C01B25/02

CPC classification number: C01B25/02 ,  B01L2200/12 ,  G01N33/48721

Abstract: The present disclosure provides black phosphorous devices with use in, e.g., inter alia, molecular analysis applications. A device may comprise a region of black phosphorous with, e.g., 1 to 10 layers and having a pore formed through the layer or layers. The black phosphorous may be supported by a support membrane. Also provided are related methods of molecular analysis and methods of fabricating the devices.

Abstract translation: 本公开提供了用于例如分子分析应用的黑磷装置。 装置可以包括具有例如1至10层的黑磷区域并且具有穿过该层或多个层形成的孔。 黑磷可以由支撑膜支撑。 还提供了相关的分子分析方法和制造器件的方法。

公开(公告)号：WO2017157784A1

公开(公告)日：2017-09-21

申请号：PCT/EP2017/055642

申请日：2017-03-10

Applicant: VESDO INC.

Inventor： TOEDTLI, Sergej

IPC: G06K19/06 ,  B01L3/00

CPC classification number: G06K19/06028 ,  B01L3/545 ,  B01L2200/12 ,  B01L2300/021 ,  G06K19/06037 ,  G06K19/06046

Abstract: The present invention relates to a receptacle (110) comprising at least one interior (114) for receiving at least one content,a completely or partially cylindrical lateral surface (116) at least partially enclosing the at least one interior (114), and a multitude of successively arranged identical machine-readable unique identifier codes (132) surrounding the lateral surface (116). The present invention further relates to a method for applying a multitude of identical machine-readable unique identifier codes (132) to a receptacle (110) having a completely or partially cylindrical lateral surface (116), wherein the identical identifier codes (132) are successively printed, thereby surrounding the lateral surface(116). The present invention further relates to a method for reading out at least one of a multitude of machine-readable unique identifier codes (132) on at least one completely or partially cylindrical lateral surface (116) of a receptacle (110), the receptacle (110) comprising a multitude of successively arranged identical machine-readable unique identifier codes (132) surrounding the lateral surface (116), wherein the at least one unique identifier code (132) is read out by a code reading camera (160) without rotating the receptacle (110).

Abstract translation: 本发明涉及一种容器（110），其包括用于接收至少一种内容物的至少一个内部（114），完全或部分圆柱形的侧表面（116），其至少部分地包围至少一个内容物 一个内部（114）以及多个连续布置的围绕所述侧表面（116）的相同的机器可读唯一标识符代码（132）。 本发明还涉及一种用于将多个相同的机器可读唯一标识码（132）应用于具有完全或部分圆柱形侧表面（116）的容器（110）的方法，其中相同的标识符编码（132）是 连续印刷，从而围绕侧表面（116）。 本发明还涉及一种用于读出在容器（110）的至少一个完全或部分圆柱形侧表面（116）上的多个机器可读唯一标识码（132）中的至少一个的方法，所述容器 110），其包括围绕所述侧表面（116）的多个连续排列的相同机器可读唯一标识码（132），其中所述至少一个唯一标识码（132）由码读取照相机（160）读出而不旋转 插座（110）。

公开(公告)号：WO2017117105A1

公开(公告)日：2017-07-06

申请号：PCT/US2016/068674

申请日：2016-12-27

Applicant: INTELLIGENT BIO-SYSTEMS, INC.

Inventor： PERROUD, Thomas, Daniel ,  PERBOST, Michel, Georges

IPC: G01N15/14 ,  G01N21/01 ,  G01N33/48 ,  G01N33/483 ,  G01N33/487 ,  G01N33/50 ,  C12Q1/68

CPC classification number: B01L3/502715 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/123 ,  B01L2300/168 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  G01N1/2813 ,  G01N21/05 ,  G01N21/6452 ,  G01N21/6458 ,  G02B21/34

Abstract: A sequencing instrument including a sequencing stage and a microscope. The sequencing stage has a reference plate with a flat reference surface. The microscope optical axis is perpendicular to the reference surface. The sequencing stage is configured to receive a flexible film having a plurality of DNA templates immobilized on a first side of the film, and to hold the flexible film against the flat reference surface with at least some of the plurality of DNA templates in an object plane that is perpendicular to the microscope's optical axis.

Abstract translation: 测序仪包括测序台和显微镜。 排序阶段有一个平坦的参考面的参考板。 显微镜光轴垂直于参考表面。 测序阶段被配置为接收具有固定在膜的第一侧上的多个DNA模板的柔性膜，并且将柔性膜保持抵靠平坦基准表面，其中多个DNA模板中的至少一些处于物平面 这是垂直于显微镜的光轴。

公开(公告)号：WO2017017032A9

公开(公告)日：2017-03-16

申请号：PCT/EP2016067578

申请日：2016-07-22

Applicant: QMICRO B V

Inventor： BURGER GERARDUS JOHANNES ,  BIJEN JOHN GERHARDUS MARIA ,  OUDEJANS DIONYSIUS ANTONIUS PETRUS ,  WEERDEN HARM JAN

IPC: B01L3/00

CPC classification number: B01L3/502707 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0874 ,  B01L2300/0887

Abstract: Substrate for a microfluidic device, comprising at least one microfluidic structure having at least one access port at an upper surface of the substrate, a raised support structure positioned on the upper surface adjacent to each access port and surrounding the access port, the raised support structure partially covering the substrate upper surface, the first raised support structure having an upper surface for receiving an adhesive for mounting a microfluidic component having at least one access port corresponding to the at least one access port of the substrate. A microfluidic device, comprising a substrate, a microfluidic component having at least one access port at a lower surface corresponding to the at least one access port of the substrate. The microfluidic component is mounted on the top of the substrate with an adhesive applied between the upper surface of the at least one first and/or second raised support structure and the lower surface of the microfluidic component.

Abstract translation: 用于微流体装置的基板，包括至少一个微流体结构，其具有在基板的上表面处的至少一个进入口;凸起的支撑结构，其位于与每个进入口相邻并围绕进入口的上表面上，凸起的支撑结构 部分地覆盖基板上表面，第一凸起支撑结构具有用于接收用于安装微流体部件的粘合剂的上表面，该微流体部件具有对应于基板的至少一个进入口的至少一个进入端口。 一种微流体装置，包括基底，微流体部件，其具有在与所述基底的所述至少一个进入口对应的下表面处的至少一个进入口。 微流体组分安装在基材的顶部上，粘合剂施加在至少一个第一和/或第二凸起支撑结构的上表面和微流体组分的下表面之间。

公开(公告)号：WO2017039752A1

公开(公告)日：2017-03-09

申请号：PCT/US2016/023262

申请日：2016-03-18

Applicant: NORTH CAROLINA STATE UNIVERSITY

Inventor： CUMMINS, Brian M. ,  LIGLER, Frances Smith ,  WALKER, Glenn

IPC: B81B1/00 ,  B81C1/00

CPC classification number: B01L3/50273 ,  B01L3/5023 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2200/06 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/126 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/084 ,  F04B37/02 ,  F04B37/04 ,  F04F99/00 ,  F05B2280/50

Abstract: Provided herein are passive microfluidic pumps. The pumps can comprise a fluid inlet, an absorbent region, a resistive region fluidly connecting the fluid inlet and the absorbent region, and an evaporation barrier enclosing the resistive region, the absorbent region, or a combination thereof. The resistive region can comprise a first porous medium, and a fluidly non-conducting boundary defining a path for fluid flow through the first porous medium from the fluid inlet to the absorbent region. The absorbent region can comprise a fluidly non-conducting boundary defining a volume of a second porous medium sized to absorb a predetermined volume of fluid imbibed from the resistive region. The resistive region and the absorbent region can be configured to establish a capillary-driven fluid front advancing from the fluid inlet through the resistive region to the absorbent region when the fluid inlet is contacted with fluid.

Abstract translation: 本文提供的是无源微流体泵。 泵可以包括流体入口，吸收区域，流体连接流体入口和吸收区域的电阻区域，以及包围电阻区域，吸收区域或其组合的蒸发屏障。 电阻区域可以包括第一多孔介质和流体非导电边界，其限定用于从流体入口到吸收区域的流体流过第一多孔介质的路径。 吸收区域可以包括流体非导电边界，限定一定体积的第二多孔介质体积，以吸收从电阻区域吸收的预定体积的流体。 电阻区域和吸收区域可以被配置为当流体入口与流体接触时，建立从流体入口通过电阻区域前进到毛细管驱动的流体前端到吸收区域。

公开(公告)号：WO2016201163A1

公开(公告)日：2016-12-15

申请号：PCT/US2016/036787

申请日：2016-06-10

Applicant: TEXAS TECH UNIVERSITY SYSTEM

Inventor： VANAPALLI, Siva, A ,  BITHI, Swastika, S.

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/12 ,  B01L2200/142 ,  B01L2300/041 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2400/049 ,  B01L2400/084

Abstract: A microfluidic device includes a substrate and a cover. The substrate has an inlet port, a first microchannel, one or more parking loops, a second microchannel and an outlet port for each microchannel network. The first microchannel is connected to the inlet port, the second microchannel is connected to the outlet port, the parking loops are connected between the first and second microchannels. Each parking loop includes a parking loop inlet, a parking loop output, a fluidic trap connected between the parking loop inlet and the parking loop outlet, and a bypass microchannel connected to the parking loop inlet and the parking loop outlet. The cover is attached to a top of the substrate and has an inlet opening and an outlet opening through the cover for each microchannel network. The inlet and outlet openings of the cover are disposed above the inlet and outlet ports in the substrate.

Abstract translation: 微流体装置包括基底和盖。 衬底具有用于每个微通道网络的入口，第一微通道，一个或多个停车回路，第二微通道和出口。 第一微通道连接到入口，第二微通道连接到出口，停车回路连接在第一和第二微通道之间。 每个停车回路包括停车回路入口，停车回路输出，连接在停车回路入口和停车回路出口之间的流体回路，以及连接到停车回路入口和停车回路出口的旁路微通道。 盖子连接到基板的顶部，并且具有通过每个微通道网络的盖子的入口开口和出口开口。 盖的入口和出口开口设置在基板中的入口和出口的上方。

公开(公告)号：WO2016172362A1

公开(公告)日：2016-10-27

申请号：PCT/US2016/028681

申请日：2016-04-21

Applicant: GENERAL AUTOMATION LAB TECHNOLOGIES, INC.

Inventor： BLAINEY, Paul C. ,  SEELY, Michael W. ,  STOCKER, Roman ,  ZENGLER, Karsten ,  CONRADSON, Scott ,  CHRISTEY, Peter ,  HALLOCK, Alexander

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: C12Q1/6853 ,  B01L3/0244 ,  B01L3/5085 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/141 ,  B01L2300/021 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/165 ,  C12M23/12 ,  C12M23/24 ,  C12Q1/6816 ,  C12Q1/689 ,  C12Q2525/161 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2547/101 ,  C12Q2563/159 ,  C12Q2563/179

Abstract: A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. A series of unique tags may be disposed in the microwells to identify one or more species of cells and locate the particular microwells in which each species was cultivated. A unique tag may be a nucleic acid molecule including a target-specific nucleotide sequence for annealing to a target nucleic acid fragment and a location-specific nucleotide sequence predetermined to identify one or more microwells. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. High throughput methods are described for cultivating, screening, and determining a relative and/or absolute abundance of cells from a sample.

Abstract translation: 描述了限定微孔的高密度阵列的微制造装置，用于从样品中培养细胞。 可以在微孔中设置一系列独特的标签以鉴定一种或多种细胞种类并定位每种种类培养的特定微孔。 唯一标签可以是核酸分子，其包括用于退火靶核酸片段的靶特异性核苷酸序列和预定识别一个或多个微孔的位置特异性核苷酸序列。 可以将该装置孵育以生长多个细胞，其可以分成分析部分和储备部分。 描述了用于培养，筛选和确定来自样品的细胞的相对和/或绝对丰度的高通量方法。