公开(公告)号：WO02098209A3

公开(公告)日：2003-09-18

申请号：PCT/US0217717

申请日：2002-06-04

Applicant: NORTHWEST PLANT BREEDING CO ,  OHTSUKA ICHIRO ,  KONZAK CALVIN F

Inventor： OHTSUKA ICHIRO ,  KONZAK CALVIN F

IPC: A01H1/02 ,  A01H5/10 ,  A01H1/00 ,  A01H5/00

CPC classification number: A01H5/10 ,  A01H1/02

Abstract: The present invention relates to the production of hybrid wheat and triticale plants and seeds via the employment of genetically-controlled cytoplasmic male sterility (CMS) and genetically-controlled fertility restoration. The genetically-controlled cytoplasmic sterility mechanism according to the present system and method utilizes genes conferring CMS in the presence of Aegilops squarrosa cytoplasm, and for restoring fertility to wheat and triticale plants having Aegilops species cytoplasm. The invention also relates to the use of specific, induced mutant sources of tolerance to herbicides for destroying the seed producing ability of the male fertility restorer parent after pollination has completed, to achieve essentially 100% hybrid seed reproduction from a mixture of a CMS male sterile line and a pollen fertility restorer line.

Abstract translation: 本发明涉及通过使用遗传控制的细胞质雄性不育（CMS）和遗传控制育性恢复来生产杂交小麦和黑小麦植物和种子。 根据本系统和方法的遗传控制细胞质不育机制利用在Aegilops squarrosa细胞质存在下赋予CMS的基因，并恢复对具有Aegilops种细胞质的小麦和黑小麦植物的生育力。 本发明还涉及特异性诱导的除草剂耐受突变来源在授粉完成后破坏雄性育性恢复亲本的种子生产能力的用途，从CMS雄性不育的混合物中获得基本上100％的杂交种子繁殖 线和花粉生育恢复系。

公开(公告)号：WO2002098209A2

公开(公告)日：2002-12-12

申请号：PCT/US2002/017717

申请日：2002-06-04

Applicant: NORTHWEST PLANT BREEDING CO. ,  OHTSUKA, Ichiro ,  KONZAK, Calvin, F.

Inventor： OHTSUKA, Ichiro ,  KONZAK, Calvin, F.

IPC: A01H

CPC classification number: A01H5/10 ,  A01H1/02

Abstract: The present invention relates to the production of hybrid wheat and triticale plants and seeds via the employment of genetically-controlled cytoplasmic male sterility (CMS) and genetically-controlled fertility restoration. The genetically-controlled cytoplasmic sterility mechanism according to the present system and method utilizes genes conferring CMS in the presence of Aegilops squarrosa cytoplasm, and for restoring fertility to wheat and triticale plants having Aegilops species cytoplasm. The invention also relates to the use of specific, induced mutant sources of tolerance to herbicides for destroying the seed producing ability of the male fertility restorer parent after pollination has completed, to achieve essentially 100% hybrid seed reproduction from a mixture of a CMS male sterile line and a pollen fertility restorer line.

Abstract translation: 本发明涉及通过使用遗传控制的细胞质雄性不育（CMS）和遗传控制育性恢复来生产杂交小麦和黑小麦植物和种子。 根据本系统和方法的遗传控制细胞质不育机制利用在Aegilops squarrosa细胞质存在下赋予CMS的基因，并恢复对具有Aegilops种细胞质的小麦和黑小麦植物的生育力。 本发明还涉及特异性诱导的除草剂耐受突变来源在授粉完成后破坏雄性育性恢复亲本的种子生产能力的用途，从CMS雄性不育的混合物中获得基本上100％的杂交种子繁殖 线和花粉生育恢复系。

公开(公告)号：WO0114518A3

公开(公告)日：2001-10-18

申请号：PCT/US0018790

申请日：2000-08-23

Applicant: NORTHWEST PLANT BREEDING CO ,  KONZAK CALVIN F ,  POLLE ENRIQUE A ,  LIU WEIGUO ,  ZHENG YUANMING

Inventor： KONZAK CALVIN F ,  POLLE ENRIQUE A ,  LIU WEIGUO ,  ZHENG YUANMING

IPC: A01H4/00 ,  C12N5/00 ,  A01H1/08 ,  A01H5/00 ,  C12N15/05 ,  C12N15/82

CPC classification number: A01H4/001 ,  A01H4/005 ,  A01H4/008

Abstract: The present invention provides methods for generating doubled haploid and/or haploid plants from microspores. The methods of the invention include the steps of (a) purifying microspores at a developmental stage amenable to androgenic induction; (b) subjecting said microspores to nutrient stress to obtain stressed microspores; (c) contacting said microspores with an amount of a sporophytic development inducer effective to induce sporophytic development, said contacting step occurring before, during, after, or overlapping with any portion of said nutrient stress step; and (d) culturing said isolated microspores with at least one live plant ovary or with an aliquot of plant ovary conditioned medium. The methods of the present invention can also include the step of genetically transforming the microspores to produce genetically transformed plants.

Abstract translation: 本发明提供了从小孢子产生加倍单倍体和/或单倍体植物的方法。 本发明的方法包括以下步骤：（a）在适于雄激素诱导的发育阶段纯化小孢子; （b）使所述小孢子受到营养应力以获得应激小孢子; （c）使所述小孢子与一定量的有效诱导孢子体发育的孢子体发育诱导剂接触，所述接触步骤在所述营养胁迫步骤的任何部分之前，之中，之后或与其重叠; 和（d）用至少一个活的植物卵巢或用等分的植物卵巢条件培养基培养所述分离的小孢子。 本发明的方法还可以包括遗传转化小孢子以产生遗传转化的植物的步骤。

公开(公告)号：WO02052926A3

公开(公告)日：2003-04-03

申请号：PCT/US0200327

申请日：2002-01-02

Applicant: NORTHWEST PLANT BREEDING CO ,  ZHENG YUANMING ,  KONZAK CALVIN F ,  WENG YUJIA ,  RAFIULLAH SAHIBZADA

Inventor： ZHENG YUANMING ,  KONZAK CALVIN F ,  WENG YUJIA ,  RAFIULLAH SAHIBZADA

IPC: A01H1/08 ,  A01H4/00 ,  A01H5/00 ,  C12N5/00 ,  C12N5/04 ,  C12N15/82

CPC classification number: A01H1/08 ,  A01H4/005 ,  A01H4/008

Abstract: In one aspect, the present invention provides methods for generating doubled haploid and/or haploid maize plants from microspores. The methods of this aspect of the invention include the steps of: (a) selecting maize plant material comprising maize microspores at a developmental stage amenable to androgenic induction; (b) incubating the microspores in incubation medium at a temperature and osmolarity effective to induce androgenesis to obtain temperature-treated microspores; (c) isolating the temperature-treated microspores; (d) cultivating the isolated, temperature-treated microspores in cultivation medium with either at least one live plant ovary and/or ovary-conditioned medium to produce regenerative maize tissue, wherein the cultivation medium has an osmolarity between about 300 mOsm and about 500 mOsm and comprises at least one cytokinin and at least one auxin; and (e) regenerating maize plants from the regenerative maize tissue. The present invention also provides methods for producing regenerative maize tissue from maize microspores.

Abstract translation: 一方面，本发明提供从小孢子中产生双重单倍体和/或单倍体玉米植物的方法。 本发明该方面的方法包括以下步骤：（a）在适于雄激素诱导的发育阶段选择包含玉米小孢子的玉米植物材料; （b）在培养基中温育和渗透压下孵育小孢子以有效诱导雄激素以获得经温度处理的小孢子; （c）分离经温度处理的小孢子; （d）在至少一个活的植物卵巢和/或卵巢条件培养基中培养分离的温度处理的小孢子，以产生再生玉米组织，其中培养基具有约300mOsm至约500mOsm的渗透压 并且包含至少一种细胞分裂素和至少一种生长素; 和（e）从再生玉米组织再生玉米植物。 本发明还提供了从玉米小孢子生产再生玉米组织的方法。

公开(公告)号：WO2002096220A1

公开(公告)日：2002-12-05

申请号：PCT/US2002/015674

申请日：2002-05-16

Applicant: NORTHWEST PLANT BREEDING CO. ,  KONZAC, Calvin, F.

Inventor： KONZAC, Calvin, F.

IPC: A23L1/0522

CPC classification number: A21D13/062 ,  A21D2/186 ,  A21D2/26 ,  A21D13/06

Abstract: In one aspect, the present invention provides partially waxy wheat flour comprising starch amylose in an amount of from 10 weight percent to 20 weight percent, and amylopectin in an amount less than 90%. In another aspect, the present invention provides flour blends comprising one or more partially waxy wheat flours of the invention. The present invention also provides baked goods prepared from a flour or flour blend of the invention. The baked goods of the invention possess an extended shelf life. In other aspects, the present invention provides methods for making baked goods.

Abstract translation: 一方面，本发明提供了含有10重量％至20重量％的淀粉直链淀粉的部分蜡质小麦粉和少于90％的支链淀粉。 另一方面，本发明提供包含本发明的一种或多种部分蜡状小麦粉的面粉混合物。 本发明还提供由本发明的面粉或面粉共混物制备的烘焙食品。 本发明的烘焙食品具有延长的保质期。 在其他方面，本发明提供了制造烘焙食品的方法。