METHODS OF PRODUCING SOMATIC HYBRID AND CYBRID ORGANISMS

    公开(公告)号:WO2023043942A1

    公开(公告)日:2023-03-23

    申请号:PCT/US2022/043688

    申请日:2022-09-15

    发明人: TUDELA, Jerred

    摘要: Methods of producing a hybrid organism. The methods include providing a first organism, providing a second organism, providing a fusing medium, combining the first organism and the second organism in or on the fusing medium to define a fusion environment, and initiating somatic fusion between the first organism and the second organism in the fusion environment to produce the hybrid organism. The first organism is either a plant organism or a fungus organism. The second organism is either a plant organism or a fungus organism. In some examples, the first organism is tuber melanosporum. In certain examples, the second organism is either panaeolus cambodginiensis or panaeolus cyanescens. in some examples, the methods include incubating the hybrid organism, regenerating cell wails of the hybrid organism, and/or replicating the hybrid organism.

    COMPOSITIONS AND METHODS FOR POLLEN STORAGE
    3.
    发明申请

    公开(公告)号:WO2022187054A1

    公开(公告)日:2022-09-09

    申请号:PCT/US2022/017506

    申请日:2022-02-23

    IPC分类号: A01H1/02 A01G7/00 A01G7/06

    摘要: The invention provides novel compositions and methods for storage and delivery of pollen to a female reproductive part of a recipient plant. The pollen storage composition provided includes at least one perlite particle and pollen. The methods provided include storing the pollen storage composition and applying the pollen storage composition to at least a first female reproductive part of a recipient plant, thereby pollinating the female reproductive part with the pollen from the donor plant.

    一种基于单株评价及全基因组选择技术的高效玉米育种方法

    公开(公告)号:WO2022166474A1

    公开(公告)日:2022-08-11

    申请号:PCT/CN2021/142034

    申请日:2021-12-28

    IPC分类号: A01H1/02 A01H1/04

    摘要: 本发明公开了一种基于单株评价及全基因组选择技术的高效玉米育种方法,该方法包括以下步骤:S1、第一种植季对玉米母本进行多父本花粉授粉;S2、第二种植季对杂交籽粒进行单粒播种,单株选择并评价目标性状;S3、对选择的杂交组合亲本进行鉴定;S4、对杂交组合的目标性状进行全基因组预测;S5、根据预测的目标性状选择优良杂交组合;S6、选择的优良杂交组合直接进入品种审定环节或继续进行评价。本发明提供的育种方法极大地减少了第一种植季杂交组合组配的种子量及第二种植季杂交组合的种植规模,有效降低了育种成本,并可对未组配的杂交组合进行预测进而筛选优良组合,减少优良遗传资源的浪费,提高育种实践的选择效率。

    一种介导调控植物隐性核雄性不育系雄性育性的GAT载体及其应用

    公开(公告)号:WO2021223726A1

    公开(公告)日:2021-11-11

    申请号:PCT/CN2021/092016

    申请日:2021-05-07

    IPC分类号: C12N15/82 A01H1/02

    摘要: 提供了一种介导调控植物隐性核雄性不育系雄性育性的GAT载体及其应用。该GAT载体包括五种功能元件表达盒:植物雄性育性恢复基因元件表达盒,用于恢复隐性核不育突变体的雄性育性;植物花粉败育基因元件表达盒,用于清除含GAT的花粉及保持GAT保持系的杂合状态;基因转化及保持系筛选元件表达盒,用于基因转化及GAT保持系除杂提纯;除草剂敏感元件表达盒,用于清除除草剂敏感的GAT保持系花粉及种子外逸及GAT不育系除杂提纯;种子筛选元件表达盒,用于种子机械分选。该GAT载体可用于植物隐性核不育材料的杂交育种和杂交制种。