公开(公告)号：WO2014190249A1

公开(公告)日：2014-11-27

申请号：PCT/US2014/039320

申请日：2014-05-23

Applicant: OCCAM BIOLABS, INC.

Inventor： QIAN, Mingwei

IPC: B01L3/14 ,  C12M1/00 ,  G01N1/34

CPC classification number: C12N15/1017 ,  B01L3/502 ,  B01L3/508 ,  B01L2200/023 ,  B01L2200/025 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/185 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/048 ,  B01L2300/0681 ,  B01L2300/0854 ,  B01L2300/105 ,  B01L2400/0409 ,  B01L2400/049 ,  B01L2400/0605 ,  B65B7/28 ,  C12Q1/6806

Abstract: A system for collecting and shipping a sample of nucleic acid, the system comprising a receptacle, a removable cap for the receptacle having a breather port and sample connection port, and a filter column removably attached to the inside of the receptacle cap in fluid communication with the sample connection port and containing a substrate for collecting the nucleic acid. A sample collection container interlocks to the sample collection port. A shipping container, closeable by a lid, is configured to detach the filter column from the receptacle cap and contain the column for shipping. Methods for collecting samples using the system preferably include a dehydrating wash step, such as with ethanol, and placement of a dessicant in the shipping container, so that nucleic acid samples can be transported under ambient, non-climate-controlled conditions, with stability for at least up to 4 weeks, ideal for collecting samples from undeveloped regions.

Abstract translation: 一种用于收集和运送核酸样品的系统，该系统包括容器，用于具有通气孔和样品连接口的容器的可拆卸盖，以及可移除地附接到容器盖内部的过滤柱，其与 样品连接口并含有用于收集核酸的底物。 样品收集容器与样品收集口互锁。 可由盖子关闭的运输容器被配置为将过滤器柱从容器盖分离，并容纳用于运输的色谱柱。 使用该系统收集样品的方法优选包括脱水洗涤步骤，例如用乙醇，并将干燥剂放置在运输容器中，使得核酸样品可以在环境，非气候条件下运输，具有稳定性 至少4周，是收集未开发地区样品的理想选择。

公开(公告)号：WO2009055438A2

公开(公告)日：2009-04-30

申请号：PCT/US2008/080744

申请日：2008-10-22

Applicant: OCCAM BIOLABS, INC. ,  QIAN, Mingwei ,  LI, Haizhang

Inventor： QIAN, Mingwei ,  LI, Haizhang

IPC: B01L9/06 ,  B01L3/14

CPC classification number: B01L9/06 ,  B01L9/54

Abstract: A container for elongated objects, such as microtubes or vials, comprising aframe having a base and sides, a pair of rotatable opposing lid sections having at least two movement-resistant positions, and a rack with a plurality of holes or collars. The rack or interior bottom of the container may have features that discourage rotation of the microtubes or vials. The containers may further comprise microtubes or vials, which may be empty or filled with, for example, reagents, such as reagents for use in a predetermined process. A system of stackable carriers may be provided to hold and allow one-handed removal of a plurality of containers. The container may desirably permit one-handed opening and closing of the container and any vials contained therein, and the various lid positions may be suitable for submersion in an ice bath as well as standing upright on a laboratory bench.

Abstract translation: 用于细长物体的容器，例如微管或小瓶，包括具有基部和侧面的aframe，一对具有至少两个可移动位置的可旋转相对的盖部分，以及具有多个孔或套环的齿条。 容器的机架或内部底部可能具有阻止微管或小瓶旋转的特征。 容器还可以包括微管或小瓶，其可以是空的或者填充有例如用于预定方法的试剂，例如试剂。 可以提供可堆叠载体的系统以保持并允许单手取走多个容器。 容器可以理想地允许容器的单手打开和关闭以及容纳在其中的任何小瓶，并且各种盖子位置可适合于浸入冰浴以及直立在实验台上。

公开(公告)号：WO2012135815A3

公开(公告)日：2012-12-27

申请号：PCT/US2012031814

申请日：2012-04-02

Applicant: OCCAM BIOLABS INC ,  QIAN MINGWEI

Inventor： QIAN MINGWEI

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/04

CPC classification number: C12Q1/689

Abstract: The present invention relates to a method for detecting a target nucleic acid derived from a pathogen in a subject. The method comprises (a) amplifying the nucleic acid sequence of the target nucleic acid, which is obtained from a cell-free fraction of a blood sample from the subject, to produce a double stranded DNA is produced, and (b) detecting the double stranded DNA. The presence of the double stranded DNA indicates the presence of the target nucleic acid in the subject. Also provided are kits for detecting a target nucleic acid derived from a pathogen in a subject.

Abstract translation: 本发明涉及用于检测受试者中源自病原体的靶核酸的方法。 该方法包括（a）扩增从受试者的血液样品的无细胞部分获得的靶核酸的核酸序列以产生双链DNA，并且（b）检测双重 搁浅的DNA。 双链DNA的存在表明受试者中存在靶核酸。 还提供了用于检测受试者中源自病原体的靶核酸的试剂盒。

公开(公告)号：WO2012135815A2

公开(公告)日：2012-10-04

申请号：PCT/US2012/031814

申请日：2012-04-02

Applicant: OCCAM BIOLABS, INC. ,  QIAN, Mingwei

Inventor： QIAN, Mingwei

IPC: C12Q1/68 ,  C12Q1/04 ,  C12N15/11

CPC classification number: C12Q1/689

Abstract: The present invention relates to a method for detecting a target nucleic acid derived from a pathogen in a subject. The method comprises (a) amplifying the nucleic acid sequence of the target nucleic acid, which is obtained from a cell-free fraction of a blood sample from the subject, to produce a double stranded DNA is produced, and (b) detecting the double stranded DNA. The presence of the double stranded DNA indicates the presence of the target nucleic acid in the subject. Also provided are kits for detecting a target nucleic acid derived from a pathogen in a subject.

Abstract translation: 本发明涉及用于检测受试者中源自病原体的靶核酸的方法。 该方法包括（a）扩增从受试者的血液样品的无细胞级分获得的目标核酸的核酸序列以产生双链DNA，和（b）检测双重 双链DNA。 双链DNA的存在表明目标核酸在受试者中的存在。 还提供了用于检测受试者中源自病原体的靶核酸的试剂盒。

公开(公告)号：WO2016081860A1

公开(公告)日：2016-05-26

申请号：PCT/US2015/061917

申请日：2015-11-20

Applicant: OCCAM BIOLABS, INC.

Inventor： QIAN, Mingwei

IPC: B01L3/14 ,  C12M1/12 ,  G01N1/34

CPC classification number: C12N15/1017 ,  B01L3/502 ,  B01L3/5082 ,  B01L2200/026 ,  B01L2200/0631 ,  B01L2200/0689 ,  B01L2200/185 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2300/123 ,  C12Q1/6806 ,  G01N1/40 ,  G01N2001/005

Abstract: A system for collecting biomolecules comprising an extractor assembly comprising a top cap, an extractor core, an extractor core adaptor, an extractor body having an internal volume, and a bottom cap ring. The top cap comprises a sample connection port and is configured to be secured to the extractor body by the bottom cap ring. The extractor core is adapted to be removably attached to a connection interface of the top cap and contains a substrate for collecting the biomolecules. The extractor core adaptor has an upstream open end for mating with the downstream end of the extractor core and a downstream protrusion configured to project through an opening in the extractor body. Methods for using the system to collect samples for transport and further processing to detect disease are also disclosed.

Abstract translation: 一种用于收集生物分子的系统，其包括提取器组件，该提取器组件包括顶盖，提取器芯，提取器芯适配器，具有内部容积的提取器主体和底盖环。 顶盖包括样品连接端口，并且被配置为通过底盖环固定到提取器主体。 提取器芯适于可移除地附接到顶盖的连接界面并且包含用于收集生物分子的基板。 提取器芯适配器具有用于与提取器芯的下游端配合的上游开口端和配置成突出通过提取器主体中的开口的下游突起。 还公开了使用该系统收集样品进行运输和进一步处理以检测疾病的方法。

公开(公告)号：WO2009055438A3

公开(公告)日：2009-07-16

申请号：PCT/US2008080744

申请日：2008-10-22

Applicant: OCCAM BIOLABS INC ,  QIAN MINGWEI ,  LI HAIZHANG

Inventor： QIAN MINGWEI ,  LI HAIZHANG

IPC: B01L9/06 ,  B01L3/14

CPC classification number: B01L9/06 ,  B01L9/54

Abstract: A container for elongated objects, such as microtubes or vials, comprising aframe having a base and sides, a pair of rotatable opposing lid sections having at least two movement-resistant positions, and a rack with a plurality of holes or collars. The rack or interior bottom of the container may have features that discourage rotation of the microtubes or vials. The containers may further comprise microtubes or vials, which may be empty or filled with, for example, reagents, such as reagents for use in a predetermined process. A system of stackable carriers may be provided to hold and allow one-handed removal of a plurality of containers. The container may desirably permit one-handed opening and closing of the container and any vials contained therein, and the various lid positions may be suitable for submersion in an ice bath as well as standing upright on a laboratory bench.

Abstract translation: 用于细长物体如微管或小瓶的容器包括具有基部和侧面的框架，具有至少两个运动阻止位置的一对可旋转的相对盖部分以及具有多个孔或环的齿条。 容器的架子或内部底部可具有阻止微管或小瓶旋转的特征。 容器可以进一步包括微管或小瓶，其可以是空的或填充有例如试剂，例如用于预定过程的试剂。 可以提供可堆叠载体的系统以保持并允许单手去除多个容器。 容器可以理想地允许单手打开和关闭容器以及容纳在其中的任何小瓶，并且各种盖子位置可以适合于浸没在冰浴中以及直立在实验室工作台上。