公开(公告)号：WO2019149765A1

公开(公告)日：2019-08-08

申请号：PCT/EP2019/052274

申请日：2019-01-30

Applicant: SCIENOVA GMBH

Inventor： KREUSCH, Stefan ,  RICHTER, Thomas ,  RUBICK, Raffael

IPC: B01L3/00

CPC classification number: B01L3/50825 ,  B01L3/50853 ,  B01L2200/0689 ,  B01L2300/023 ,  B01L2300/046 ,  B01L2300/048 ,  B01L2300/0618 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2300/0858

Abstract: Die Erfindung beinhaltet funktionelle vertikale Einwegreaktionssysteme mit vertikal angebrachter semipermeabler Membran für Probenvorbereitung, chemische Reaktionen, Dialyse, enzymatische/mikrobiologische Fermentation, Mehrstufenprozesse, in vitro-Protein-Biosynthese im Labormaßstab, gebildet aus einem Grundkörper und einem wechselbaren Deckel mit unterschiedlichen Funktionen. Das System wird für den Austausch über die Membran vertikal in ein Außenvolumen bestehend aus Gas, Flüssigkeit oder festen Bestandteilen gesetzt. Das System besteht aus einem formstabilen Grundkörper und einem flüssigkeitsdichten Deckel mit einem Funktionsträger, der zum Boden des Grundkörpers reicht, wobei der formstabile Grundkörper mindestens einen nichtkapillaren Reaktionsraum als Innenvolumen mit mindestens einer semipermeablen Membran als Seitenwand bildet. Die hohe Einsatzflexibiliät ergibt sich aus der Kombination von Varianten der Grundkörper mit verschiedenen Deckelvarianten für unterschiedliche Einsatzgebiete. Die Grundkörper mit verschiedenen Membranen und Volumina können mit Deckeln mit verschiedenen Beschickungsöffnungen, Kontakten, Sensorträgern, Begasungen, Umwälzmöglichkeiten etc. gekoppelt werden. Damit ergeben sich bei m verschiedenen Grundkörpern und n verschiedenen Deckelvarianten m x n Kombinationen mit verschiedenen Eigenschaften.

公开(公告)号：WO2019121445A1

公开(公告)日：2019-06-27

申请号：PCT/EP2018/085109

申请日：2018-12-17

Applicant: TECAN TRADING AG

Inventor： OTT, Philipp ,  ERB, Dominic

IPC: B01L3/02 ,  G01N35/10 ,  G01F23/26

CPC classification number: B01L3/021 ,  B01L2200/143 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0681 ,  G01F23/263 ,  G01N35/1016 ,  G01N35/109 ,  G01N2035/102 ,  G01N2035/1025

Abstract: The present invention pertains to a pipetting apparatus and method capable of detecting a liquid (9, 9') within an intermediate section (4) of a pipette tube (1) of the pipetting apparatus. The intermediate section (4) is located between an upper section (2) of the pipette tube (1) at which a first electrode (10) is arranged and a lower section (3) at which a second electrode (11) is arranged. The first and second electrodes (10, 11) form a measurement capacitor and are operationally connected to an impedance measurement unit (13), which is adapted to detect whether liquid (9, 9'), such as a portion of a sample liquid (9) or system liquid (9'), is present within the intermediate section (4) based on the measured impedance or change of impedance, e.g. an increase of the capacitance and/or a decrease of the resistance, of the measurement capacitor caused by a presence of the liquid (9, 9') within the intermediate section (4).

公开(公告)号：WO2019113457A1

公开(公告)日：2019-06-13

申请号：PCT/US2018/064494

申请日：2018-12-07

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： LOVE, J., Christopher ,  GIERAHN, Todd, Michael ,  SHALEK, Alexander, K. ,  WADSWORTH, Marc, Havens ,  HUGHES, Travis, K.

IPC: B01L3/00 ,  C12Q1/6806 ,  C12N15/10 ,  C12M1/12 ,  G03F7/00

CPC classification number: C12Q1/6874 ,  B01L3/5085 ,  B01L3/50855 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6806 ,  G03F7/2014 ,  C12Q2525/15 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2563/179 ,  C12Q2525/173

Abstract: This disclosure describes improvements to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, improved portability and ease of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays.

公开(公告)号：WO2019005809A1

公开(公告)日：2019-01-03

申请号：PCT/US2018/039522

申请日：2018-06-26

Applicant: BOSTON SCIENTIFIC SCIMED, INC.

Inventor： KENWOOD, Shannon Smith ,  HEIGHTON, Sarah Jean ,  CLARK, Joshua ,  BACON, Dan

IPC: B01L3/00 ,  C12M1/00 ,  G01N1/40

CPC classification number: B04B5/0442 ,  B01D21/262 ,  B01D61/147 ,  B01D61/18 ,  B01D2221/10 ,  B01D2311/2676 ,  B01L3/5021 ,  B01L2200/025 ,  B01L2300/0618 ,  B01L2300/0681 ,  B01L2400/0409 ,  B04B2005/0478 ,  C12M47/02 ,  G01N1/4077 ,  G01N2001/4088

Abstract: Filtration devices and methods for collecting, filtering, and/or processing biological samples are disclosed. An example filtration device may include a funnel member having a first end region and a second end region. A flange may be coupled to the first end region. A filter support portion may be coupled to the second end region. The filter support portion may include a base, a filter membrane coupled to the base, and a securing member for securing the filter membrane to the base.

公开(公告)号：WO2018183985A1

公开(公告)日：2018-10-04

申请号：PCT/US2018/025606

申请日：2018-03-31

Applicant: INDIANA BIOSCIENCES RESEARCH INSTITUTE

Inventor： PUGIA, Michael, Joseph ,  BAIRD, Zane ,  CAO, Zehui

IPC: C12M3/06 ,  B01D29/88 ,  B01D35/00

CPC classification number: G01N1/4077 ,  B01L3/50273 ,  B01L3/5055 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2400/0457 ,  B01L2400/0463 ,  B01L2400/049 ,  G01N27/3277 ,  G01N2001/4088

Abstract: The invention provides a method of releasing a liquid from a porous matrix having at least one pore to a microfluidic surface having at least one liquid volume area and at least one exit hole, comprising: (a) filtering said liquid through said porous matrix; (b) removing said porous matrix and sealing the microfluidic surface having a liquid volume area; (c) releasing said liquid from the liquid volume area through the exit hole of the microfluidic surface by application of a dynamic force; and (d) collecting said liquid into a liquid receiving area. Additionally, there is provided an apparatus useful for processing liquid samples undergoing analytical assays which includes (a) a filtering device having a porous matrix afixed to a support structure; (b) a microfluidic surface having a liquid volume area and an exit hole connected to said filtering device; and (c) a liquid receiving area attached to said microfluidic surface.

公开(公告)号：WO2018138701A1

公开(公告)日：2018-08-02

申请号：PCT/IB2018/050544

申请日：2018-01-30

Applicant: KALANTARINEJAD, Reza

Inventor： KALANTARINEJAD, Reza ,  YAGHOOBZADEH TARI, Abdolreza ,  MERATI, Mahmoud Reza ,  AFRAZ, Ahmadreza ,  YOUSEFI, Soroor ,  JABI, Vahid ,  BANIAHMAD, Faranak ,  RASTIKERDAR, Mahsa ,  PASHA ZANOOSI, Ali Akbar ,  MEYGHANI, Parisa ,  AGHAZADEH, Mohammad Amin

IPC: A61B5/00 ,  G16H10/00

CPC classification number: B01L3/502715 ,  B01L3/502753 ,  B01L2200/147 ,  B01L2300/022 ,  B01L2300/025 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0605 ,  B01L2400/0666 ,  G01N33/53

Abstract: A point-of-care medical testing system integrated with a patient monitor is disclosed. The system may include a microfluidic cartridge configured to receive a blood sample and generate a sensory signal dependent on a concentration of a biomarker in the blood sample. A cartridge reading assembly receives the microfluidic cartridge. The cartridge reading assembly includes a processing unit and a memory coupled with the processing unit. The memory stores executable instructions to cause the processing unit to receive the sensory signal, correlate the received sensory signal with the concentration of the biomarker in the blood sample, and produce an output representative of the concentration of the biomarker in the blood sample. The cartridge reading assembly is coupled to the patient monitor and configured to send the output to the patient monitor.

公开(公告)号：WO2018048873A2

公开(公告)日：2018-03-15

申请号：PCT/US2017/050236

申请日：2017-09-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： CHAO, Philip Hong-Sean ,  VAN DAM, R. Michael

IPC: A61K51/04 ,  A61M1/00

CPC classification number: A61K51/02 ,  A61B6/037 ,  A61K51/0446 ,  A61K51/0459 ,  A61K51/0491 ,  B01L3/502753 ,  B01L2300/0681 ,  B01L2300/0883 ,  B01L2300/12 ,  C07B59/00

Abstract: A method of formulating or concentrating a radiolabeled molecule or compound includes providing a microfluidic device having a sample layer containing a microfluidic channel formed therein, a porous membrane having a pore size of less than 0.5 µm disposed on the sample layer and covering the microfluidic channel, and a gas flow layer having a gas-carrying channel formed therein, wherein the porous membrane is interposed between the sample layer and the gas flow layer. A fluid containing the radiolabeled molecule or compound is delivered into the microfluidic channel. Heat is applied to evaporate the fluid. A gas is passed through gas-carrying channel to remove evaporated fluid from the microfluidic device.

Abstract translation: 配制或浓缩放射性标记的分子或化合物的方法包括提供微流体装置，所述微流体装置具有其中形成有微流体通道的样品层，孔径小于0.5μm的多孔膜 在样品层上并覆盖微流体通道，以及在其中形成有气体携带通道的气流层，其中多孔膜置于样品层和气流层之间。 含有放射性标记的分子或化合物的液体被输送到微流体通道中。 施加热量以蒸发流体。 气体通过载气通道从微流体装置中除去蒸发的流体。

公开(公告)号：WO2017197343A2

公开(公告)日：2017-11-16

申请号：PCT/US2017/032525

申请日：2017-05-12

Applicant: 10X GENOMICS, INC.

Inventor： BERNATE, Jorge ,  BHARADWAJ, Rajiv ,  MASQUELIER, Donald, A. ,  WHEELER, Tobias, Daniel

IPC: B01D25/12

CPC classification number: B01L3/502753 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2300/021 ,  B01L2300/0681 ,  B01L2300/0816 ,  C12M23/16 ,  C12M25/04 ,  C12N1/02 ,  C12N11/04

Abstract: A microfiuidic system for manipulating particles in a fluid is provided. The system includes a microfiuidic chip having at least one channel and at least one filter feature, wherein the filter feature includes a field of physical obstacles configured and arranged to remove physical contaminants associated with the particles. The filter features are particularly suited to remove contaminants associated with gel beads manipulated by microfiuidic handling.

Abstract translation: 提供了用于处理流体中的粒子的微流体系统。 该系统包括具有至少一个通道和至少一个过滤器特征的微流体芯片，其中过滤器特征包括被配置和布置成去除与颗粒相关联的物理污染物的物理障碍物场。 过滤器特点特别适用于去除与微流体处理操作的凝胶珠相关的污染物。

公开(公告)号：WO2017084754A1

公开(公告)日：2017-05-26

申请号：PCT/EP2016/001925

申请日：2016-11-18

Applicant: SINAMIRA AG ,  FRIEDRICH-ALEXANDER-UNIVERSITÄT ERLANGEN-NÜRNBERG

Inventor： MIEDL, Walter ,  FRIEDRICH, Oliver ,  GILBERT, Daniel

IPC: G01N33/569 ,  G01N21/00 ,  B01L3/00

CPC classification number: G01N33/56911 ,  B01L3/502 ,  B01L3/545 ,  B01L2300/021 ,  B01L2300/022 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  Y02A50/52 ,  Y02A50/57 ,  Y02A50/59

Abstract: Die vorliegende Erfindung betrifft ein schnelles, einfaches und sehr sensitives Verfahren zum Nachweis von Bakterien, umfassend die Schritte: Bereitstellung einer oder mehrerer Suspensionen, jeweils umfassend mindestens eine Spezies von markierten Testbakteriophagen, welche spezifisch an eine nachzuweisende Bakterienart bindet; Zugabe einer Probe, die auf das Vorliegen mindestens einer nachzuweisenden Bakterienart getestet werden soll, zu der einen oder den mehreren Suspensionen; Filtration des Reaktionsgemisches; Detektion von Bakterien-Bakteriophagen-Komplexen auf der Filteroberfläche im Retentat, bei Vorliegen mindestens einer nachzuweisenden Bakterienart, wobei die Komplexe aus Bakterien der mindestens einen nachzuweisenden Bakterienart und daran gebundenen Testbakteriophagen der mindestens einen Spezies von Testbakteriophagen bestehen; Detektion von ungebundenen Testbakteriophagen im Filtrat; prozessorgestützte Verarbeitung von erhaltenen Detektionssignalen und Ausgabe von Detektionsergebnissen. Ferner werden ein Reaktionsgefäß sowie ein Messgerät für das Verfahren offenbart.

Abstract translation:

本发明涉及一种用于检测细菌，其包括如下步骤的快速，简便和高度敏感的方法，包括：提供一个或多个悬浮液，每一个都包括其在细菌物种特异性检测标记Testbakteriophagen中的至少一个物种 结合; 将要检测的样品添加到所述一种或多种悬浮液中以检测待检测的至少一种细菌物种; 过滤反应混合物; 待检测车在保留物中，在至少一种细菌物种的存在，其特征在于，要被检测和至少键合Testbakteriophagen由一个物种Testbakteriophagen的由至少一种细菌物种的细菌的复合物;对Filteroberfl＆AUML细菌 - 噬菌体复合物检测; 检测滤液中未结合的测试噬菌体; 处理器辅助处理接收到的检测信号并输出​​检测结果。 此外，反应容器|？ 和一个方法的米。

公开(公告)号：WO2017062901A3

公开(公告)日：2017-05-26

申请号：PCT/US2016056172

申请日：2016-10-07

Applicant: TYMORA ANALYTICAL OPERATIONS LLC

Inventor： TAO ANDY ,  ILYUK ANTON ,  BAO NING

IPC: C12M1/42 ,  C12N13/00 ,  G01N27/00 ,  G01N30/00 ,  G01N33/50 ,  H03K3/64

CPC classification number: C12M1/34 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  C12M23/16 ,  C12M33/00 ,  C12M47/16 ,  C12Q1/00

Abstract: EVs are connected to onset and progression of numerous diseases and can be used for their diagnosis. Methods and platform technology to isolate EVs and detect intravesicle phosphoproteins from biofluids are discussed. Secreted extracellular vesicles in biofluids are effectively isolated and phosphoproteins are extracted for detection and identification as biomarkers for disease diagnosis using antibodies, mass spectrometry, or other like methods.

Abstract translation: 电动车与许多疾病的发作和进展有关，可用于诊断。 讨论了分离电动汽车和检测生物体内液体磷脂蛋白的方法和平台技术。 有效分离生物流体中分泌的胞外囊泡，并提取磷蛋白用于检测和鉴定，作为使用抗体，质谱法或其他类似方法进行疾病诊断的生物标志物。