公开(公告)号：WO2006065076A1

公开(公告)日：2006-06-22

申请号：PCT/KR2005/004308

申请日：2005-12-14

Applicant: CJ CORPORATION ,  PAN, Jae-Gu ,  JUNG, Heung-Chae ,  KIM, Eui-Joong ,  LEE, Han-Seung ,  PARK, Young Hoon ,  KIM, Hyoung Suk ,  HAN, Jong-Kwon ,  LEE, Jin Nam ,  OH, Ki Hoon ,  KIM, Jeong Hwan ,  OH, Yoon Suk ,  SIM, Jae Ick ,  HONG, Kuk-Ki ,  CHOI, Kyung Oh ,  KIM, Hyun Soo ,  BAEK, Min-Ji ,  KANG, Tae Sun

Inventor： PAN, Jae-Gu ,  JUNG, Heung-Chae ,  KIM, Eui-Joong ,  LEE, Han-Seung ,  PARK, Young Hoon ,  KIM, Hyoung Suk ,  HAN, Jong-Kwon ,  LEE, Jin Nam ,  OH, Ki Hoon ,  KIM, Jeong Hwan ,  OH, Yoon Suk ,  SIM, Jae Ick ,  HONG, Kuk-Ki ,  CHOI, Kyung Oh ,  KIM, Hyun Soo ,  BAEK, Min-Ji ,  KANG, Tae Sun

IPC: C12N9/00 ,  C12N15/52

CPC classification number: C12N9/93

Abstract: The present invention relates to the development of 5'- xanthylic acid (XMP) aminase mutants having enhanced activity using artificial mutagenesis. The 5'-XMP aminase mutants were obtained by performing error-prone PCR to introduce mutations into the native 5'-XMP aminase gene, screening the resulting mutant library for highly active 5'-XMP aminase, performing molecular evolution using the obtained highly active 5'-XMP aminase mutant as a parent gene to obtain 5 ' -XMP aminase having much higher activity, and determining nucleotide sequences of the resulting mutants. The 5'-XMP aminase mutants effectively convert 5 ' -XMP to 5 ' -guanylic acid because they have higher activity than the native form.

Abstract translation: 本发明涉及使用人工诱变增强活性的5'-叶酸（XMP）氨基酶突变体的开发。 通过进行易错PCR引入天然5'-XMP氨基酶基因的突变获得5'-XMP氨基酸突变体，筛选得到的高活性5'-XMP氨基酶的突变文库，使用获得的高活性进行分子进化 5'-XMP氨基酶突变体作为亲本基因以获得具有高得多活性的5'-XMP氨基酶，并测定所得突变体的核苷酸序列。 5'-XMP氨基酶突变体有效地将5'-XMP转化为5'-鸟苷酸，因为它们具有比天然形式更高的活性。

公开(公告)号：WO2007069861A1

公开(公告)日：2007-06-21

申请号：PCT/KR2006/005475

申请日：2006-12-14

Applicant: CJ CORPORATION ,  PAN, Jae-Gu ,  JUNG, Heung-Chae ,  KIM, Eui-Joong ,  LEE, Han-Seung ,  PARK, Young Hoon ,  KIM, Hyoung Suk ,  HAN, Jong-Kwon ,  LEE, Jin Nam ,  OH, Ki-Hoon ,  KIM, Jeong Hwan ,  OH, Yoon-Suk ,  SIM, Jae Ick ,  HONG, Kuk-Ki ,  CHOI, Kyung Oh ,  KIM, Hyun Soo ,  BAEK, Min-Ji ,  KANG, Tae Sun

Inventor： PAN, Jae-Gu ,  JUNG, Heung-Chae ,  KIM, Eui-Joong ,  LEE, Han-Seung ,  PARK, Young Hoon ,  KIM, Hyoung Suk ,  HAN, Jong-Kwon ,  LEE, Jin Nam ,  OH, Ki-Hoon ,  KIM, Jeong Hwan ,  OH, Yoon-Suk ,  SIM, Jae Ick ,  HONG, Kuk-Ki ,  CHOI, Kyung Oh ,  KIM, Hyun Soo ,  BAEK, Min-Ji ,  KANG, Tae Sun

IPC: C12N1/00

CPC classification number: C12N9/78 ,  C12P19/32

Abstract: Disclosed herein are ammonia-specific 5'-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5'-XMP aminase. The resulting 5'-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5'- XMP into 5'-GMP. Thus, the ammonia-specific 5'-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5'-XMP into 5'-GMP.

Abstract translation: 本文公开了氨特异性5'-XMP氨基酶突变体及其制备方法。 在5'-XMP氨基酶中引入谷氨酰胺依赖性催化活性位点的突变。 所得到的5'-XMP氨基酶突变体缺乏谷氨酰胺依赖性活性，并特异性地与外部氨反应将5'- XMP转化为5'-GMP。 因此，与野生型相比，氨特异性5'-XMP氨基酶突变体在细胞内更稳定，可用于将5'-XMP工业转化为5'-GMP。

公开(公告)号：WO2006059877A1

公开(公告)日：2006-06-08

申请号：PCT/KR2005/004080

申请日：2005-12-01

Applicant: CJ Corporation ,  PARK, Young Hoon ,  KIM, Hyoung Suk ,  LEE, Jin Nam ,  OH, Ki Hoon ,  KIM, Jeong Hwan ,  OH, Yoon Suk ,  SIM, Jae Ick ,  CHOI, Kyung Oh

Inventor： PARK, Young Hoon ,  KIM, Hyoung Suk ,  LEE, Jin Nam ,  OH, Ki Hoon ,  KIM, Jeong Hwan ,  OH, Yoon Suk ,  SIM, Jae Ick ,  CHOI, Kyung Oh

IPC: C12N1/20

CPC classification number: C12P19/32 ,  C12R1/15

Abstract: Disclosed is a mutant strain of Corynebacterium ammoniagenes CJXFT0301 (KCCM-10530) , Corynebacterium aimoniagenes CJXSul0401 (KCCM-10609) , which produces 5'- xanthylic acid in high concentrations and high yields by direct fermentation. Also disclosed is a method of producing 5 ' -xanthylic acid, which is based on directly accumulating 5 '-xanthylic acid in culture fluid in high concentrations and high yields using the mutant strain. The present microorganism shares the characteristics of the CJXFT0301 (KCCM-10530) strain, requirement for adenine and guanine, susceptibility to lysozymes and resistance to praline analogues, glutamine analogues and tryptophan analogues. Due to its sulfadiazine resistance, leading to increased synthesis of dihydropteroate and eventually elevated levels of tetrahydrofolic acid, the present microorganism directly accumulates 5 ' -xanthylic acid in culture fluid in high concentrations and high yields during the same fermentation duration.

Abstract translation: 公开了产氨棒杆菌CJXFT0301（KCCM-10530），目标棒杆菌CJXSul0401（KCCM-10609）的突变菌株，其通过直接发酵以高浓度和高产率产生5'-叶酸。 还公开了一种生产5'-戊酸的方法，其基于使用突变菌株以高浓度和高产量直接在培养液中积累5'-戊酸。 本发明的微生物分享了CJXFT0301（KCCM-10530）菌株的特征，对腺嘌呤和鸟嘌呤的需求，对溶菌酶的敏感性和对脯氨酸类似物，谷氨酰胺类似物和色氨酸类似物的抗性。 由于其磺胺嘧啶耐药性，导致二氢芥酸酯的合成增加并最终提高了四氢叶酸的水平，本发明的微生物在相同的发酵时间内以高浓度和高收率直接在培养液中积累5'-戊酸。

公开(公告)号：WO2005056775A1

公开(公告)日：2005-06-23

申请号：PCT/KR2004/002993

申请日：2004-11-18

Applicant: CJ CORP. ,  PARK, Young-Hoon ,  CHANG, Jea-Young ,  LEE, Jin-Nam ,  OH, Ki-Hoon ,  KIM, Jeong-Hwan ,  OH, Yoon-Suk ,  SIM, Jae-Ick

Inventor： PARK, Young-Hoon ,  CHANG, Jea-Young ,  LEE, Jin-Nam ,  OH, Ki-Hoon ,  KIM, Jeong-Hwan ,  OH, Yoon-Suk ,  SIM, Jae-Ick

IPC: C12N1/20

CPC classification number: C12P19/32 ,  C12R1/15 ,  Y10S435/822 ,  Y10S435/843

Abstract: The invention relates to a microorganism, obtained by treating Corynebacterium ammoniagenes KCCM 10488 producing 5’- Xanthylic acid as parent strain with UV radiation and mutation Derivatives such as N-methyl-N’nitro-N-nitrosoguanidine (NTG), Having a resistance to 5-fluorotryptophan which enhances Biosynthesis of N5-N10- tetrahydrofolate used for transferring Two formyl group during the process of puring biosysthesis, making It possible to accumulate 5’xanthylic acid in culture medium at a high Yield and high concentration rate same period of fermentation.

Abstract translation: 本发明涉及通过用UV辐射和突变衍生物如N-甲基-N'-硝基-N-亚硝基胍（NTG）处理产生5'-叶酸作为亲本菌株的产氨杆菌KCCM10488得到的微生物，具有抗 5-fluorotryptophan，其增强用于转移的N5-N10-四氢叶酸的生物合成。在生物合成过程中两个甲酰基，使得可以在相同发酵时期的高产率和高浓度下在培养基中积累5'叶酸。

公开(公告)号：WO2004053109A1

公开(公告)日：2004-06-24

申请号：PCT/KR2003/002703

申请日：2003-12-10

Applicant: CJ CORPORATION ,  KWAG, Young-Hyeon ,  OH, Ki-Hoon ,  KIM, Jeong-Hwan ,  OH, Yoon-Suk ,  SIM, Jae-Ick ,  PARK, Young-Hoon ,  CHANG, Jea-Young

Inventor： KWAG, Young-Hyeon ,  OH, Ki-Hoon ,  KIM, Jeong-Hwan ,  OH, Yoon-Suk ,  SIM, Jae-Ick ,  PARK, Young-Hoon ,  CHANG, Jea-Young

IPC: C12N1/20

CPC classification number: C12P19/32 ,  C12P19/40 ,  C12R1/15

Abstract: The invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 producing 5'-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to oligomycin. In order to obtain mutant strain having enhanced respiratory activity, the present invention adopted Corynebacterium ammoniagenes KCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N'-nitro-n-nitrosoguanidine(NTG) according to ordinary procedure. Therefore, Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 of the present invention makes it possible to increase ATP reproducing activity for same period of fermentation and can accumulate 5'xanthylic acid in culture medium at a high yield and concentration rate.

Abstract translation: 本发明涉及产生5'-戊酸的产氨棒杆菌CJXOL 0201 KCCM 10447。 更具体地，本发明涉及产氨杆菌CJXOL 0201 KCCM 10447，它是具有对寡霉素抗性的产氨棒杆菌KCCM10340的突变菌株。 为了获得具有增强的呼吸活性的突变菌株，本发明使用产氨杆菌KCCM 10340作为亲本菌株，并用UV辐射和N-甲基-N'-硝基-N-亚硝基胍（NTG）等突变衍生物按照 普通程序。 因此，本发明的产氨酸棒杆菌CJXOL 0201 KCCM 10447可以在相同的发酵期内增加ATP的再生活性，并且可以以高产率和浓度的速率在培养基中积累5'-戊酸。

公开(公告)号：WO2008048017A1

公开(公告)日：2008-04-24

申请号：PCT/KR2007/004970

申请日：2007-10-11

Applicant: CJ CHEILJEDANG CORPORATION ,  JIN, Chang hyun ,  OH, Ki-Hoon ,  CHO, Kwang Myung ,  PARK, Young Hoon

Inventor： JIN, Chang hyun ,  OH, Ki-Hoon ,  CHO, Kwang Myung ,  PARK, Young Hoon

IPC: C12N1/21 ,  C12P1/04 ,  C12P13/14

CPC classification number: C12P13/14 ,  C12N15/01

Abstract: Disclosed herein are mutant strains, KCCM- 10784P and KCCM- 10785P, which are obtained through gene manipulation of Corynebacterium glutamicum KFCC-11074, and a process of producing L-glutamic acid using the mutant strains. The mutant strains are capable of producing L-Glutamic acid at high yield.

Abstract translation: 本文公开了通过谷氨酸棒杆菌KFCC-11074的基因操作获得的突变菌株KCCM-10784P和KCCM-10785P，以及使用突变菌株产生L-谷氨酸的方法。 突变株能以高产率生产L-谷氨酸。

公开(公告)号：WO2006078132A1

公开(公告)日：2006-07-27

申请号：PCT/KR2006/000221

申请日：2006-01-20

Applicant: CJ CORP. ,  PARK, Young-hoon ,  KIM, Hyoung-suk ,  LEE, Jin-nam ,  OH, Ki-hoon ,  KIM, Jeong-hwan ,  OH, Yoon-suk ,  SIM, Jae-ick ,  CHOI, Kyung-oh

Inventor： PARK, Young-hoon ,  KIM, Hyoung-suk ,  LEE, Jin-nam ,  OH, Ki-hoon ,  KIM, Jeong-hwan ,  OH, Yoon-suk ,  SIM, Jae-ick ,  CHOI, Kyung-oh

IPC: C12N1/20

CPC classification number: C12N9/16 ,  C12N9/1077 ,  C12P19/32 ,  C12R1/19

Abstract: Provided are mutant strains derived from Escherichia sp. GPU1114 (Accession No. KCCM-10536), having cumulative inactivation of deoD, aphA , appA , and hprt genes, and methods of using the same.

Abstract translation: 提供源自大肠杆菌属的突变菌株。 具有deoD，aphA，appA和hprt基因的累积失活的GPU1114（登录号KCCM-10536）及其使用方法。

公开(公告)号：WO2004053110A1

公开(公告)日：2004-06-24

申请号：PCT/KR2003/002704

申请日：2003-12-10

Applicant: CJ CORPORATION ,  KWAG, Young-Hyeon ,  OH, Ki-Hoon ,  KIM, Jeong-Hwan ,  OH, Yoon-Suk ,  SIM, Jae-Ick ,  PARK, Young-Hoon ,  CHANG, Jea-Young

Inventor： KWAG, Young-Hyeon ,  OH, Ki-Hoon ,  KIM, Jeong-Hwan ,  OH, Yoon-Suk ,  SIM, Jae-Ick ,  PARK, Young-Hoon ,  CHANG, Jea-Young

IPC: C12N1/20

CPC classification number: C12P17/182 ,  C12R1/15

Abstract: The invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 producing 5'-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to osmotic pressure. In order to obtain mutant strain having enhanced respiratory activity, the present invention adopted Corynebacterium ammoniagenes KCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N'-nitro-n-nitrosoguanidine(NTG) according to ordinary procedure. Therefore, Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 of the present invention makes it possible to overcome growth-standing phase rapidly on early culture and for same period of fermentation, can accumulate 5'-xanthylic acid in culture medium at a high yield and concentration rate.

Abstract translation: 本发明涉及产生5'-戊酸的产氨棒杆菌CJXSP 0201 KCCM 10448。 更具体地，本发明涉及具有抗渗透压的耐氨基酸棒杆菌KCCM 10340的突变菌株的产氨棒杆菌CJXSP 0201 KCCM 10448。 为了获得具有增强的呼吸活性的突变菌株，本发明使用产氨杆菌KCCM 10340作为亲本菌株，并用UV辐射和N-甲基-N'-硝基-N-亚硝基胍（NTG）等突变衍生物按照 普通程序。 因此，本发明的产氨酸棒杆菌CJXSP 0201 KCCM 10448可以在早期培养中快速克服生长稳定期，并且在相同的发酵期内，可以以高产率和浓度的速率在培养基中积累5'-叶酸。