公开(公告)号：WO2022060728A1

公开(公告)日：2022-03-24

申请号：PCT/US2021/050262

申请日：2021-09-14

Applicant: PALAMEDRIX, INC.

Inventor： GOPINATH, Ashwin ,  ROTHEMUND, Paul ,  SHETTY, Rishabh ,  BOWEN, Shane

IPC: B82Y5/00 ,  B82Y40/00 ,  C12Q1/6834 ,  G01N33/487 ,  G01N33/543

Abstract: Provided herein are structures and methods for detecting one or more analyte molecules present in a sample. In some embodiments, the one or more analyte molecules are detected using one or more supramolecular structures. In some embodiments, the one or more supramolecular structures are specifically designed to minimize cross-reactivity with each other. In some embodiments, the supramolecular structures are bi-stable, wherein the supramolecular structures shift from an unstable state to a stable state through interaction with one or more analyte molecules from the sample. In some embodiments, the stable state supramolecular structures are configured to provide a signal for analyte molecule detection and quantification. In some embodiments, the signal correlates to a DNA signal, such that detection and quantification of an analyte molecule comprises converting the presence of the analyte molecule into a DNA signal.

公开(公告)号：WO2023049355A1

公开(公告)日：2023-03-30

申请号：PCT/US2022/044548

申请日：2022-09-23

Applicant: PALAMEDRIX, INC.

Inventor： GOPINATH, Ashwin

IPC: C12Q1/6804 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68

Abstract: Provided herein in various embodiments, is a method for detecting and/or quantifying an analyte molecule present in a sample without employing a sequencing operation. As discussed, monofunctional particles are used to perform the detection and/or quantification of the analyte of interest. In one embodiment the monofunctional particles include a supramolecular structure (e.g., a nucleic acid origami structure) that comprises a core structure composed of one or more core molecules, a single affinity binder linked to the supramolecular structure at a first location, and one or more unique identifiers also attached to the supramolecular structure and which convey information about the affinity binder present on a respective monofunctional particle.

公开(公告)号：WO2022251514A1

公开(公告)日：2022-12-01

申请号：PCT/US2022/031155

申请日：2022-05-26

Applicant: PALAMEDRIX, INC.

Inventor： GOPINATH, Ashwin ,  ROTHEMUND, Paul ,  SHETTY, Rishabh ,  BOWEN, Shane ,  GALIMIDI, Rachel ,  YUAN, Dajun

IPC: G01N33/543 ,  C12Q1/68

Abstract: Provided herein are structures and methods for detecting one or more analyte molecules present in a sample. In some embodiments, the one or more analyte molecules form a complex in solution with a supramolecular structure. The supramolecular structures of the complex may be detectable such that binding of the analyte molecule to a binding site of an array is detectable via one or more features of the supramolecular structure. A binding site of an array includes capture molecules to capture bound complexes to facilitate detection.

公开(公告)号：WO2022212479A2

公开(公告)日：2022-10-06

申请号：PCT/US2022/022515

申请日：2022-03-30

Applicant: PALAMEDRIX, INC.

Inventor： BOWEN, Shane ,  ROTHEMUND, Paul ,  GOPINATH, Ashwin

IPC: C12N15/10 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N33/53 ,  C12Q1/6804 ,  G01N33/54386 ,  G16B50/20

Abstract: Provided herein are structures and methods for detecting one or more analyte molecules present in a sample. In some embodiments, the one or more analyte molecules are detected using one or more supramolecular structures. In some embodiments, the supramolecular structures are bi-stable, wherein the supramolecular structures shift from an unstable state to a stable state through interaction with one or more analyte molecules from the sample. In some embodiments, the stable state supramolecular structures are configured to provide a signal for analyte molecule detection and quantification.

公开(公告)号：WO2022182643A1

公开(公告)日：2022-09-01

申请号：PCT/US2022/017281

申请日：2022-02-22

Applicant: PALAMEDRIX, INC.

Inventor： GOPINATH, Ashwin ,  ROTHEMUND, Paul ,  SHETTY, Rishabh ,  BOWEN, Shane ,  GALIMIDI, Rachel

IPC: G01N33/543 ,  C12Q1/68

Abstract: Provided herein are structures and methods for detecting one or more analyte molecules present in a sample. In some embodiments, the one or more analyte molecules are detected using one or more supramolecular structures. In some embodiments, the supramolecular structures facilitate binding of a single detector molecule. In some embodiments, the stable state supramolecular structures are configured to provide a signal for analyte molecule detection and quantification. In some embodiments, the signal correlates to a DNA signal, such that detection and quantification of an analyte molecule comprises converting the presence of the analyte molecule into a DNA signal.

公开(公告)号：WO2022182635A1

公开(公告)日：2022-09-01

申请号：PCT/US2022/017256

申请日：2022-02-22

Applicant: PALAMEDRIX, INC.

Inventor： GOPINATH, Ashwin ,  ROTHEMUND, Paul ,  SHETTY, Rishabh ,  BOWEN, Shane ,  GALIMIDI, Rachel

IPC: C12Q1/6834 ,  C12Q1/6837 ,  G01N33/50 ,  C08G83/00

Abstract: Provided herein are structures and methods for detecting one or more analyte molecules present in a sample. In some embodiments, the one or more analyte molecules are detected using one or more supramolecular structures. In some embodiments, the supramolecular structures are configured to form a linkage with a particular capture barcode, which is configured to form a linkage with a particular captur molecule. In some embodiments the capture molecule is configured to interact with a particular analyte molecule. In some embodiments, the locations of supramolecular structures are mapped on a substrate having a plurality of binding locations, according to the capture barcode and/or another barcode linked with the supramolecular structures. In some embodiments, the linkage between the analyte molecules and supramolecular structures enable a signal to be generated. In some embodiments, the signal generated enables the identification and quantification of the analyte molecules in the sample based on the mapped location of the supramolecular structures on the substrate.

公开(公告)号：WO2022115756A2

公开(公告)日：2022-06-02

申请号：PCT/US2021/061130

申请日：2021-11-30

Applicant: PALAMEDRIX, INC.

Inventor： GOPINATH, Ashwin ,  ROTHEMUND, Paul ,  SHETTY, Rishabh ,  BOWEN, Shane

IPC: C12Q1/6825 ,  G01N33/53 ,  G01N27/4145 ,  G01N33/5308

Abstract: Provided herein are structures and methods for detecting one or more analyte molecules 44 present in a sample. In some embodiments, the one or more analyte molecules 44 are detected using one or more supramolecular structures 40 that are coupled to a substrate, e.g., a solid support. In some embodiments, the supramolecular structures 40 are bi-stable, wherein the supramolecular structures 40 transition from an unstable state to a stable state through interaction with one or more analyte molecules 44 from the sample. In some embodiments, the stable state supramolecular structures 40 are configured to provide a signal for analyte molecule detection and quantification.