公开(公告)号：WO2002059575A2

公开(公告)日：2002-08-01

申请号：PCT/US2002/002144

申请日：2002-01-23

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY, PRINCETON UNIVERSITY ,  SOHN, Lydia, L. ,  SALEH, Omar, A.

Inventor： SOHN, Lydia, L. ,  SALEH, Omar, A.

IPC: G01N15/12

CPC classification number: B82Y30/00 ,  G01N15/1056 ,  G01N15/1227 ,  G01N2015/1254 ,  G01N2015/149

Abstract: A quantitative microchip Coulter counter is provided for use in detecting and measuring particles or macromolecules. The device comprises a conduit, a fluid handling system, and a measurement system. Preferably, the conduit is at least in part formed from an elastomeric material.

Abstract translation: 定量微芯片库尔特计数器用于检测和测量颗粒或大分子。 该装置包括导管，流体处理系统和测量系统。 优选地，导管至少部分地由弹性体材料形成。

公开(公告)号：WO2023086670A2

公开(公告)日：2023-05-19

申请号：PCT/US2022/049950

申请日：2022-11-15

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： MYHRVOLD, Cameron ,  KIM, Gaeun ,  ARIZTI-SANZ, Jon ,  MCNULTY, Ryan

IPC: C12Q1/689

Abstract: Described herein is a method of screening for a nuclease with an altered nuclease activity comprising: a) forming a first compartmentalizing reaction comprising a carrier and a variant nuclease template nucleic acid comprising a coding region for a variant nuclease, and amplifying said coding region for said variant nuclease, to obtain variant nuclease encoding nucleic acid; b) forming a second compartmentalizing reaction comprising said variant nuclease encoding nucleic acid, and performing an in vitro transcription and translation reaction to obtain variant nuclease polypeptides; and c) forming a third compartmentalizing reaction comprising said variant nuclease polypeptides, and assaying said variant nuclease polypeptides for the altered nuclease activity of said nuclease.

公开(公告)号：WO2023044422A1

公开(公告)日：2023-03-23

申请号：PCT/US2022/076556

申请日：2022-09-16

Applicant: TRUSTEES OF PRINCETON UNIVERSITY

Inventor： MCBRIDE, Carolyn S. ,  ZHAO, Zhilei

IPC: A01N31/02 ,  A01N35/02

Abstract: Mosquito attractant compositions that mimic the neural activity evoked by real human odor in the mosquito brain. The attractant compositions comprise 1-hexanol and linear long chain aldehydes that mimic human attractant odors and that differentiate humans from other animals. Methods for controlling malaria and dengue virus transmission as well as other diseases that are transmitted using mosquitos as vectors are included as are general methods and devices for attracting, killing, and controlling populations of mosquitos.

公开(公告)号：WO2023018877A1

公开(公告)日：2023-02-16

申请号：PCT/US2022/040073

申请日：2022-08-11

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： ACOSTA, Ellen ,  GITAI, Zemer

IPC: C12Q1/689

Abstract: A highly reliable and accurate method of assessing bacterial community viability has been developed that allows for the assessment of extremely low biomass samples, which cannot be done with traditional methods, such as qPCR. The method utilizes both PMA and droplet digital PCR (PMA-ddPCR), resulting in very accurate quantification of DNA even at very low abundances. Comparing DNA abundance in untreated samples to DNA abundance in PMA- treated samples allows the calculation of the overall viability of bacteria in any given sample. Further, PMA can be combined with traditional RNA gene sequencing (using gene-specific primers for a target species or strain) to accurately profile, e.g., the human skin microbiome, which has previously been done using traditional sequencing methods alone, but this method allows for a species-level understanding of the viable (and nonviable) components of any complex bacterial community.

公开(公告)号：WO2023017495A1

公开(公告)日：2023-02-16

申请号：PCT/IB2022/057592

申请日：2022-08-13

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY ,  INDIAN INSTITUTE OF SCIENCE

Inventor： RAJAN, Ananth Govind ,  MARTIREZ, John Mark P ,  CARTER, Emily A

IPC: C01B3/04 ,  C01B3/02

Abstract: The present disclosure relates to an efficient method of water splitting for large/industrial scale production of oxygen at the anode in an electrolyzer, while at the same time permitting hydrogen production at the cathode of the same electrolyzer. More particularly, the present disclosure provides optimal operating conditions such as temperature, pressure, and pH for a range of potentials for an electrochemical water splitting reactor to generate the maximum amount of oxygen at a given voltage while supplying the minimum possible amount of alkali.

公开(公告)号：WO2023011514A1

公开(公告)日：2023-02-09

申请号：PCT/CN2022/109906

申请日：2022-08-03

Applicant: VERSITECH LIMITED ,  THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： LIU, Yuan ,  SHEN, Yanting ,  SHUM, Anderson Ho Cheung ,  NUNES, Janine, K. ,  STONE, Howard, A.

IPC: A61L26/00 ,  A61L24/00

Abstract: Disclosed is a fiber-based hydrogel containing an aqueous hydrogel containing biocompatible microfibers having a high aspect ratio length to diameter suspended in water, the biocompatible microfibers mechanically interlocked. By simply adjusting the fiber aspect ratio, the fiber-based hydrogels can tune a considerable range of mechanical properties and drug release rates for clinical use. The fiber-based hydrogel has favorable biocompatibility and accelerated tissue regeneration ability demonstrated by a mice excision skin model.

公开(公告)号：WO2022159642A2

公开(公告)日：2022-07-28

申请号：PCT/US2022/013213

申请日：2022-01-21

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： BRANGWYNNE, Clifford, P. ,  JUMPER, Chanelle ,  ACKERMAN, Paul ,  BRACHA, Dan

IPC: G01N33/50 ,  G01N33/68 ,  C12N15/85

Abstract: Provided herein are methods of identifying an inhibitory activity of an exogeneous agent that disrupts binding of a target peptide to a biomolecule using phase separation as in cellulo read-out. Provided herein also are kits for the methods, pharmaceutical compositions comprising the agents screened by the methods, methods of preparing the pharmaceutical composition, and methods of treating a disease or condition in a subject in need thereof by administering the pharmaceutical composition.

公开(公告)号：WO2022125620A1

公开(公告)日：2022-06-16

申请号：PCT/US2021/062338

申请日：2021-12-08

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY [US]/[US] ,  THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, A BODY CORPORATE AND POLITIC OF THE STATE OF ILLINOIS [US]/[US]

Inventor： SEYEDSAYAMDOST, Mohammad, R. ,  FEDERLE, Michael, J. ,  COVINGTON, Brett, C. ,  BUSHIN, Leah, B. ,  RUED, Britta, E. ,  AMBROSE, John

IPC: C07C259/06 ,  C07C313/04 ,  C07C317/28

Abstract: There is currently a high demand for new therapeutic antibiotics with new mechanisms of action. Disclosed are two small molecules, tryglysin A and B, with novel chemical structures and potent antibiotic activity against a narrow spectrum of bacteria. This narrow spectrum of activity indicates the tryglysins could be working through a novel antibiotic mechanism of action. Due to this narrow spectrum, the tryglysins could be used as highly targeted therapeutics to treat or prevent disease without disturbing other important, "beneficial" bacteria within the human microbiome, which is a great improvement over virtually all other clinically used antibiotics that are broad-spectrum. The tryglysins are potent against several streptococcal pathogens, including Streptococcus pneumonia, the leading cause of pneumonia, and Streptococcus mutans, the primary causative agent of tooth decay and gum disease. The tryglycins may allow the development of therapeutics for the treatment and/or prevention of disease caused by these streptococci.

公开(公告)号：WO2022103784A1

公开(公告)日：2022-05-19

申请号：PCT/US2021/058698

申请日：2021-11-10

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： BOELE, Henk-Jan ,  WANG, Samuel, S.-H.

IPC: A61B5/11 ,  A61B5/00 ,  A61B5/0205 ,  A61B5/0478 ,  G06F3/01

Abstract: A system and method for neurobehavioral testing, including eyeblink conditioning and prepulse inhibition, without air puffs, that utilizes an electronic device with a light source, a camera, and a speaker, and makes assessments based on the degree to which an eyelid is closed after a user is exposed to conditional and unconditional stimuli.

公开(公告)号：WO2022094146A1

公开(公告)日：2022-05-05

申请号：PCT/US2021/057142

申请日：2021-10-28

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY ,  ZARZHITSKY, Shlomo

Inventor： HECHT, Michael

IPC: C07K14/62 ,  C12N15/11 ,  C12P21/02 ,  C12N9/64 ,  C12N15/81

Abstract: The disclosure provides, in various embodiments, methods of converting a precursor protein ( e.g., a prohormone) to a mature protein (e.g, a hormone), and methods of obtaining a target protein (e.g, from a fusion protein), using a KEX2 protease. In various embodiments, the disclosure further provides fusion proteins, polynucleotides, vectors, host cells and kits that are useful for performing the methods of the disclosure.