摘要:
A microfluidic routing device (1) for routing objects of interest (2) in a microfluidic flow, the device comprising a substrate (3); a first layer (4) provided on the substrate (3), in which the first layer (4) forms a bottom wall of a microfluidic channel (6), wherein at least two holes through the first layer (4) form respectively an inlet (11) and an outlet (12) for the microfluidic channel (6); a second layer (7) spaced away from the first layer (4), in which the second layer (7) forms a top wall of the microfluidic channel (6), wherein said second layer (7) is adapted for transmitting an optical signal from the microfluidic channel (6). The device comprises an actuator (13) for actuating said objects of interest (2) in a sorting junction of the microfluidic channel (6).
摘要:
Provided are methods and systems for reference object assisted droplet detection, indexing, and sorting in assays and diagnostics. Provided are compositions of reference objects and methods of usages. Provided are systems and exemplary modules and functions for the detection, counting, indexing, data processing, and sorting of reference objects and assay droplets in a microfluidic device. The reference objects may serve as a dynamic micro-scale positioning system for the indexing and sorting of individual assay droplets.
摘要:
Spatially distributed optical excitation and integrated waveguides are used for ultrasensitive particle detection based on individual electrokinetic velocities of particles. In some embodiments, chip-integrated systems are used to identify individual particles (e.g., individual molecules) based on their velocity as they move through an optically interrogated channel. Molecular species may be identified and quantified in a fully integrated setting, allowing for particle analysis including molecular analysis that can operate at low copy numbers down to the level of single-cell lysates. In some embodiments, the single-particle velocimetry-based identification and/or separation techniques are applied to various diagnostic assays, including nucleic acids, metabolites, macromolecules, organelles, cell, synthetic markers, small molecules, organic polymers, hormones, peptides, antibodies, lipids, carbohydrates, inorganic and organic microparticles and nanoparticles, whole viruses, and any combination thereof.
摘要:
A flowcell for a sequencing instrument. The flowcell includes a fluid inlet, a fluid outlet, a flow channel formed between an at least partially transparent cover and a base and fluidly connecting the fluid inlet to the fluid outlet, and a capture substrate provided in the flow channel. The capture substrate includes microretainers configured to each receive a single microspot having a microspot diameter, and microretainer is separated from adjacent microretainers by an interstitial gap distance that is equal to or greater than the microspot diameter. A particle separator may be fluidly connected to the flowcell. The particle separator may include a microfluidic channel having an array of micropillars to transfer a plurality of the microspots to a loading buffer that may be delivered to the flowcell.
摘要:
Aspects of the present disclosure include methods and systems for determining drop delay of a flow stream in a flow cytometer. Methods according to certain embodiments include obtaining a first frequency (f1) of drop perturbation of a flow stream subjected to an oscillating vibration, capturing one or more images of the flow stream in a detection field, obtaining a second frequency (f2) of drop perturbation of the flow stream based on one or more of the captured images and determining the drop delay of the flow stream based on the first frequency and the second frequency. Systems for practicing the subject methods having an imaging sensor for capturing one or more images of the flow stream and a processor configured to calculate drop delay using one or more of the captured images are also provided. Non-transitory computer readable storage mediums are also described.
摘要:
Die Erfindung betrifft ein Verfahren zum Überführen magnetisch markierter Partikel (36) von einem ersten in einen zweiten Medienstrom, bei dem die magnetisch markierten Partikel (36) zusammen mit dem ersten Medienstrom und der zweite Medienstrom in einen Durchflusskanal (12) eingeleitet werdenund unter Ausbildung einer Grenzfläche (22) laminar durch den Durchflusskanal (12) strömen,wobei die magnetisch markierten Partikel (36) mittels eines Magnetfeldgradienten in einer ersten Richtung (z) zu einem Kanalboden (14) des Durchflusskanals (12) hin abgelenkt werden. Die in der ersten Richtung abgelenkten magnetisch markierten Partikel (36) bewegen sich in Annäherung an den Kanalboden (14) in Strömungsrichtung weiter und werden mittels wenigstens einer an dem Kanalboden (14) angeordneten Barriere (32) in einer zweiten Richtung (x) quer zur Strömungsrichtung und quer zur Grenzfläche (22) so weit abgelenkt, dass sie aus dem ersten Medienstrom in den zweiten Medienstrom übergehen. Die Erfindung betrifft außerdem eine Vorrichtung zum Ausführen des Verfahrens.
摘要:
Compositions and methods for isolating patient -derived antigen-specific T cells include an antigen complex having a polynucleotide barcoded nanoparticle sorting agent complexed with a peptide-loaded streptavidin major histocompatability complex (MHC) tetramer, the barcoding technology allowing for high fidelity screening of a library of the antigen complexes to readily isolate and identify antigen-specific T cells.
摘要:
The present disclosure provides a method of optically marking and sorting adherent cells. In some aspects, the method comprises: providing a plurality of adherent cells attached to a substrate, each adherent cell of the plurality of adherent cells comprising an optical marker; selectively applying light energy to a subset of the plurality of adherent cells; detaching the plurality of adherent cells from the substrate; and sorting the plurality of adherent cells.