公开(公告)号：WO2009070822A3

公开(公告)日：2009-06-11

申请号：PCT/AT2008/000433

申请日：2008-12-05

Applicant: ANGEWANDTE BIOKATALYSE KOMPETENZZENTRUM GMBH ,  TECHNISCHE UNIVERSITÄT GRAZ ,  JANESCH, Bettina ,  GLIEDER, Anton ,  HARTNER, Franz ,  SCHROER, Kirsten ,  LUEF, Klaus Peter ,  PSCHEIDT, Beate

Inventor： JANESCH, Bettina ,  GLIEDER, Anton ,  HARTNER, Franz ,  SCHROER, Kirsten ,  LUEF, Klaus Peter ,  PSCHEIDT, Beate

IPC: C12N15/63 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12P7/16 ,  C12R1/84

Abstract: The present invention relates to a recombinant Pichia pastoris cell comprising at least one mutation in the dihydroxyacetone synthase gene 2 (DAS2) resulting in a reduced dihydroxyacetone synthase activity compared to a wild-type Pichia pastoris cell, wherein the DAS2 gene comprises a nucleotide sequence having at least 80% identity with SEQ ID No. 1.

公开(公告)号：WO2009070822A2

公开(公告)日：2009-06-11

申请号：PCT/AT2008000433

申请日：2008-12-05

Applicant: ANGEWANDTE BIOKATALYSE KOMPETE ,  UNIV GRAZ TECH ,  JANESCH BETTINA ,  GLIEDER ANTON ,  HARTNER FRANZ ,  SCHROER KIRSTEN ,  LUEF KLAUS PETER ,  PSCHEIDT BEATE

Inventor： JANESCH BETTINA ,  GLIEDER ANTON ,  HARTNER FRANZ ,  SCHROER KIRSTEN ,  LUEF KLAUS PETER ,  PSCHEIDT BEATE

IPC: C12N15/63 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12P7/16 ,  C12R1/84

CPC classification number: C12R1/84 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12P7/18

Abstract: The present invention relates to a recombinant Pichia pastoris cell comprising at least one mutation in the dihydroxyacetone synthase gene 2 (DAS2) resulting in a reduced dihydroxyacetone synthase activity compared to a wild-type Pichia pastoris cell, wherein the DAS2 gene comprises a nucleotide sequence having at least 80% identity with SEQ ID No. 1.

Abstract translation: 本发明涉及与野生型巴斯德毕赤酵母细胞相比，二羟基丙酮合成酶基因2（DAS2）中至少有一个突变的重组巴斯德毕赤酵母细胞，其具有降低的二羟基丙酮合成酶活性，其中DAS2基因包含具有 与SEQ ID No.1一致至少80％。