公开(公告)号：WO2011068998A2

公开(公告)日：2011-06-09

申请号：PCT/US2010/058771

申请日：2010-12-02

Applicant: DUKE UNIVERSITY ,  RAMANUJAM, Nirmala ,  PHELPS, Janelle, E.

Inventor： RAMANUJAM, Nirmala ,  PHELPS, Janelle, E.

IPC: G01N33/49 ,  G01N21/47 ,  G01N33/72 ,  A61B5/1455

CPC classification number: G01N21/49 ,  A61B5/0059 ,  A61B5/0077 ,  G01N21/314 ,  G01N21/4738 ,  G01N2201/129

Abstract: Systems and methods for determining hemoglobin concentration utilizing diffuse reflectance at isosbestic wavelengths are provided. In one embodiment, the method includes emitting light onto a tissue mass, measuring diffuse reflectance from the tissue mass, and calculating a ratio of diffuse reflectance intensities at two isosbestic wavelengths using the measured diffuse reflectance. The method also includes determining a hemoglobin concentration associated with the tissue mass by applying the calculated ratio to a predefined analytical expression associated with the two isosbestic wavelengths.

Abstract translation: 提供了利用等吸收波长漫反射确定血红蛋白浓度的系统和方法。 在一个实施例中，该方法包括将光发射到组织块上，测量来自组织块的漫反射率，并且使用测量的漫反射率计算两个等吸收波长处的漫反射强度的比率。 该方法还包括通过将计算的比率应用于与两个等吸收波长相关联的预定义的分析表达式来确定与组织质量相关联的血红蛋白浓度。

公开(公告)号：WO2010042249A2

公开(公告)日：2010-04-15

申请号：PCT/US2009/041732

申请日：2009-04-24

Applicant: DUKE UNIVERSITY ,  YU, Bing ,  RAMANUJAM, Nirmala ,  LO, Justin, Y.

Inventor： YU, Bing ,  RAMANUJAM, Nirmala ,  LO, Justin, Y.

IPC: A61B5/00 ,  A61B5/1455

CPC classification number: A61B5/0059

Abstract: A diffuse reflectance spectroscopy system for quantifying electromagnetic absorption and scattering in a tissue is provided. Also provided are optical probes and methods for imaging a tissue mass. In some embodiments, the methods include the steps of contacting a tissue mass with an optical probe, wherein the optical probe includes at least one entity for emitting light that interacts with a tissue mass and then is remitted to a collecting entity, for collecting the light that has interacted with the tissue mass, wherein the collecting entity comprises a detector comprising one or more photodiodes; measuring turbid spectral data of the tissue mass using the optical probe; converting the turbid spectral data to at least one of absorption and scattering spectral data via a Monte Carlo algorithm or a diffusion algorithm; and quantifying tissue compositions and scatterer size in a tissue mass using the at least one of absorption and scattering spectral data.

Abstract translation: 提供了用于量化组织中的电磁吸收和散射的漫反射光谱系统。 还提供了用于成像组织块的光学探针和方法。 在一些实施例中，所述方法包括使组织块与光学探针接触的步骤，其中所述光学探针包括用于发射与组织块相互作用的光的至少一个实体，然后被传送到收集实体，用于收集光 其与所述组织块相互作用，其中所述收集实体包括检测器，所述检测器包括一个或多个光电二极管; 使用光学探针测量组织块的混浊光谱数据; 通过蒙特卡罗算法或扩散算法将混浊光谱数据转换成吸收和散射光谱数据中的至少一个; 以及使用吸收和散射光谱数据中的至少一个来定量组织块中的组织组成和散射体大小。

公开(公告)号：WO2009043050A2

公开(公告)日：2009-04-02

申请号：PCT/US2008/078194

申请日：2008-09-29

Applicant: DUKE UNIVERSITY ,  RAMANUJAM, Nirmala ,  YU, Bing ,  BROWN, Quincy, J. ,  SHANG, Allan, B.

Inventor： RAMANUJAM, Nirmala ,  YU, Bing ,  BROWN, Quincy, J. ,  SHANG, Allan, B.

IPC: A61B8/00 ,  G01N21/27

CPC classification number: G01N21/4795 ,  A61B5/0059 ,  G01N21/6456 ,  G01N21/6486 ,  G01N2021/4747 ,  G01N2021/6484

Abstract: The subject matter described herein relates to an optical assay system having a multi-probe imaging array that orients a plurality of probes with respect to one another and to a sample. According to one aspect, the subject matter described herein includes a multi-probe imaging array for contacting biological samples and simultaneously illuminating a plurality of locations on the biological sample and collecting the reflected radiation from the locations. The multi-probe imaging array can be used for the rapid imaging of biological samples, for example, during surgery.

Abstract translation: 这里描述的主题涉及具有多探针成像阵列的光学测定系统，该多探针成像阵列将多个探针相对于彼此和样本定向。 根据一个方面，本文描述的主题包括用于接触生物样本并且同时照射生物样本上的多个位置并从这些位置收集反射的辐射的多探针成像阵列。 多探头成像阵列可用于生物样本的快速成像，例如在手术过程中。

公开(公告)号：WO2010042249A4

公开(公告)日：2010-08-05

申请号：PCT/US2009041732

申请日：2009-04-24

Applicant: UNIV DUKE ,  YU BING ,  RAMANUJAM NIRMALA ,  LO JUSTIN Y

Inventor： YU BING ,  RAMANUJAM NIRMALA ,  LO JUSTIN Y

IPC: A61B5/00 ,  A61B5/1455

CPC classification number: A61B5/0059

Abstract: A diffuse reflectance spectroscopy system for quantifying electromagnetic absorption and scattering in a tissue is provided. Also provided are optical probes and methods for imaging a tissue mass. In some embodiments, the methods include the steps of contacting a tissue mass with an optical probe, wherein the optical probe includes at least one entity for emitting light that interacts with a tissue mass and then is remitted to a collecting entity, for collecting the light that has interacted with the tissue mass, wherein the collecting entity comprises a detector comprising one or more photodiodes; measuring turbid spectral data of the tissue mass using the optical probe; converting the turbid spectral data to at least one of absorption and scattering spectral data via a Monte Carlo algorithm or a diffusion algorithm; and quantifying tissue compositions and scatterer size in a tissue mass using the at least one of absorption and scattering spectral data.

Abstract translation: 提供了用于量化组织中的电磁吸收和散射的漫反射光谱系统。 还提供了用于成像组织块的光学探针和方法。 在一些实施例中，所述方法包括使组织块与光学探针接触的步骤，其中所述光学探针包括至少一个用于发射光的实体，所述光与组织块相互作用，然后汇集到收集实体，用于收集光 其已经与组织块相互作用，其中收集实体包括包含一个或多个光电二极管的检测器; 使用光学探针测量组织块的浑浊光谱数据; 通过蒙特卡罗算法或扩散算法将浑浊光谱数据转换为吸收光谱数据和散射光谱数据中的至少一个; 以及使用吸收光谱数据和散射光谱数据中的所述至少一个量化组织组成和散射体大小。

公开(公告)号：WO2007109126A3

公开(公告)日：2008-03-27

申请号：PCT/US2007006624

申请日：2007-03-16

Applicant: UNIV DUKE ,  PALMER GREGORY M ,  RAMANUJAM NIRMALA

Inventor： PALMER GREGORY M ,  RAMANUJAM NIRMALA

IPC: A61B5/00 ,  F21V9/16 ,  G01T1/10

CPC classification number: G01J3/4406 ,  A61B5/0071 ,  A61B5/0084 ,  G01N21/49 ,  G01N21/6486 ,  G01N2021/6417

Abstract: The subject matter described herein includes a method for modeling fluorescence in turbid media and methods and systems for using the model to determine intrinsic fluorescence of turbid media. According to one aspect, a method for modeling fluorescence of a turbid medium and for using the model to determine intrinsic fluorescence in the turbid medium is provided. The method includes illuminating a turbid medium of interest with an electromagnetic radiation source using a probe of a particular geometry and detecting measured fluorescence for the turbid medium using the probe. At least one set of Monte Carlo simulations is run to determine an escape energy probability map and an absorbed energy density map for the turbid medium. An indication of the intrinsic fluorescence of the turbid medium is determined using the escape probability density map and the absorbed energy density map in a manner that accounts for the geometry of the probe.

Abstract translation: 本文描述的主题包括用于对混浊介质中的荧光进行建模的方法以及使用该模型确定混浊介质的固有荧光的方法和系统。 根据一个方面，提供了一种用于对浑浊介质的荧光进行建模和使用该模型来确定混浊介质中的固有荧光的方法。 该方法包括使用特定几何形状的探针用电磁辐射源照射感兴趣的混浊介质，并使用探针检测浊度介质的测量荧光。 运行至少一组蒙特卡罗模拟来确定浑浊介质的逃逸能量概率图和吸收的能量密度图。 使用逃避概率密度图和吸收的能量密度图以考虑探针的几何形状的方式确定混浊介质的固有荧光的指示。

公开(公告)号：WO2005087092A1

公开(公告)日：2005-09-22

申请号：PCT/US2005/005781

申请日：2005-02-22

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION ,  RAMANUJAM, Nirmala ,  ZHU, Changfang ,  LUBAWY, Carmalyn

Inventor： RAMANUJAM, Nirmala ,  ZHU, Changfang ,  LUBAWY, Carmalyn

IPC: A61B5/00

CPC classification number: A61B5/0075 ,  A61B5/0071 ,  A61B5/0084 ,  A61B5/0086 ,  A61B5/0091 ,  A61B5/4312 ,  A61B5/6848 ,  A61B10/0275 ,  A61B90/36 ,  A61B2017/00061 ,  A61B2090/3614

Abstract: A needle biopsy includes the step of inserting an optical spectroscopy in the needle and gathering optical information through a window formed in the side of the needle at its distal end. The optical probe (16) includes an illumination optical fiber (12) which conveys light to the tissues adjacent the side window (27) and a detection optical fiber (20) which collects light from the same tissues and conveys it to an optical spectroscopy instrument (18). Based on the results of the optical spectroscopy measurement, the optical probe may be withdrawn from the needle and a cutter advanced to acquire a sample of the tissues adjacent the sidewindow.

Abstract translation: 针穿刺活检包括将光学光谱插入针中并通过在其侧端形成的窗口收集光学信息的步骤。 光探头（16）包括向邻近侧窗（27）的组织传送光的照明光纤（12）和从相同组织收集光并将其传送到光谱仪器的检测光纤（20） （18）。 基于光谱测量的结果，可以从针中取出光学探针，并且切割器前进以获取邻近侧窗的组织的样品。

公开(公告)号：WO1997048331A1

公开(公告)日：1997-12-24

申请号：PCT/US1997010729

申请日：1997-06-19

Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM ,  RICHARDS-KORTUM, Rebecca ,  RAMANUJAM, Nirmala ,  MAHADEVAN-JANSEN, Anita ,  MITCHELL, Michele, Follen

Inventor： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

IPC: A61B05/00

CPC classification number: G01N21/6486 ,  A61B5/0059 ,  A61B5/7264 ,  G01N21/645 ,  G01N2021/6419 ,  G01N2021/6484

Abstract: The present invention involves the use of fluorescence spectroscopy in the diagnosis of cervical cancer and precancer. Using multiple illumination wavelengths, it is possible to (i) differentiate normal or inflamed tissue from squamous intraepithelial lesions (SILs) and (ii) to differentiate high grade SILs from non-high grade SILs. The detection may be performed in vitro or in vivo. Multivariate statistical analysis was employed to reduce the number of fluorescence excitation-emission wavelength pairs needed to re-develop algorithms that demonstrate a minimum decrease in classification accuracy. Fluorescence at excitation-emission wavelength pairs was used to redevelop and test screening and diagnostic algorithms that have a similar classification accuracy to those that employ fluorescence emission spectra at three excitation wavelengths. Both the full-parameter and reduced-parameter screening algorithms discriminate between SILs and non-SILs with a similar specificity and a substantially improved sensitivity relative to Pap smear screening and differentiate high grade SILs from non-high grade SILs.

Abstract translation: 本发明涉及在宫颈癌和癌前期诊断中使用荧光光谱。 使用多个照明波长，可以（i）区分正常或发炎的组织与鳞状上皮内病变（SIL）和（ii）区分高等级SIL和非高等级SILs。 检测可以在体外或体内进行。 采用多元统计分析方法，减少重新开发算法所需的荧光激发 - 发射波长对数，证明其分类精度最小。 激发 - 发射波长对的荧光被用于重建和测试筛选和诊断算法，其具有与使用三个激发波长的荧光发射光谱相似的分类精度。 全参数和简化参数筛选算法都区分SIL和非SILs，具有相似的特异性，并且相对于Pap涂片筛选具有显着改善的敏感性，并且区分高等级SIL和非高级SILs。

公开(公告)号：WO2009132360A3

公开(公告)日：2010-02-18

申请号：PCT/US2009041857

申请日：2009-04-27

Applicant: UNIV DUKE ,  YU BING ,  RAMANUJAM NIRMALA

Inventor： YU BING ,  RAMANUJAM NIRMALA

IPC: G01B11/00 ,  G01N21/00

CPC classification number: G01J3/02 ,  G01J3/0218 ,  G01J3/28 ,  G01J2003/2866 ,  G01N21/274 ,  G01N21/474 ,  G01N21/49 ,  G01N2201/082

Abstract: Systems and methods for performing optical spectroscopy using a self- calibrating fiber optic probe are disclosed. One self-calibrating fiber optic probe includes a sensing channel for transmitting illumination light to a specimen and for collecting spectral data of the specimen. The spectral data includes the illumination light diffusely reflected from the specimen at one or more wavelengths. The self-calibrating fiber optic probe may also include a calibration channel for transmitting calibration light. The calibration light and the illumination light are generated simultaneously from a common light source. The calibration channel collects calibration spectral data associated with the calibration light contemporaneously with the collection of the spectral data of the specimen.

Abstract translation: 公开了使用自校准光纤探针执行光谱的系统和方法。 一个自校准光纤探针包括用于将照明光传输到样本并用于收集样本的光谱数据的感测通道。 光谱数据包括在一个或多个波长处从样本漫反射的照明光。 自校准光纤探针还可以包括用于传送校准光的校准通道。 校准光和照明光从公共光源同时产生。 校准通道与样品的光谱数据的收集同时收集与校准光相关联的校准光谱数据。

公开(公告)号：WO2009132360A2

公开(公告)日：2009-10-29

申请号：PCT/US2009/041857

申请日：2009-04-27

Applicant: DUKE UNIVERSITY ,  YU, Bing ,  RAMANUJAM, Nirmala

Inventor： YU, Bing ,  RAMANUJAM, Nirmala

IPC: G01N13/10 ,  G01B11/00 ,  G01N21/00 ,  G12B21/02

CPC classification number: G01J3/02 ,  G01J3/0218 ,  G01J3/28 ,  G01J2003/2866 ,  G01N21/274 ,  G01N21/474 ,  G01N21/49 ,  G01N2201/082

Abstract: Systems and methods for performing optical spectroscopy using a self- calibrating fiber optic probe are disclosed. One self-calibrating fiber optic probe includes a sensing channel for transmitting illumination light to a specimen and for collecting spectral data of the specimen. The spectral data includes the illumination light diffusely reflected from the specimen at one or more wavelengths. The self-calibrating fiber optic probe may also include a calibration channel for transmitting calibration light. The calibration light and the illumination light are generated simultaneously from a common light source. The calibration channel collects calibration spectral data associated with the calibration light contemporaneously with the collection of the spectral data of the specimen.

Abstract translation: 公开了使用自校准光纤探针执行光谱的系统和方法。 一个自校准光纤探针包括用于将照明光传输到样本并用于收集样本的光谱数据的感测通道。 光谱数据包括在一个或多个波长处从样本漫反射的照明光。 自校准光纤探针还可以包括用于传送校准光的校准通道。 校准光和照明光从公共光源同时产生。 校准通道与样品的光谱数据的收集同时收集与校准光相关联的校准光谱数据。

公开(公告)号：WO2007126827A2

公开(公告)日：2007-11-08

申请号：PCT/US2007/007586

申请日：2007-03-29

Applicant: DUKE UNIVERSITY ,  RAMANUJAM, Nirmala ,  WILKE, Lee, G.

Inventor： RAMANUJAM, Nirmala ,  WILKE, Lee, G.

IPC: G01N33/53

CPC classification number: G01N21/4738

Abstract: The subject matter described herein includes an optical assay system for intraoperative assessment of tumor margins. According to one aspect, the subject matter described herein includes a biological sample containment and illumination apparatus for holding a biological sample for illumination by a plurality of electromagnetic radiation probes. The biological sample containment and illumination apparatus includes a plurality of frame members positioned with respect to each other to form an interior space for receiving a biological sample. At least one of the plurality of frame members includes a plurality of probe receiving locations for receiving a plurality of electromagnetic radiation probes. The probe receiving locations position the probes with respect to the biological sample to allow illumination of plural locations of the biological sample by the probes.

Abstract translation: 本文所述的主题包括用于术中评估肿瘤边缘的光学测定系统。 根据一个方面，本文所述的主题包括用于通过多个电磁辐射探针保持用于照明的生物样品的生物样品容纳物和照明装置。 生物样品容纳和照明装置包括相对于彼此定位的多个框架构件，以形成用于接收生物样品的内部空间。 多个框架构件中的至少一个包括用于接收多个电磁辐射探针的多个探针接收位置。 探针接收位置使探针相对于生物样品定位，以允许通过探针照射生物样品的多个位置。