公开(公告)号：WO2021222610A2

公开(公告)日：2021-11-04

申请号：PCT/US2021/029953

申请日：2021-04-29

Applicant: RAPID PATHOGEN SCREENING, INC.

Inventor： SAMBURSKY, Robert P.

IPC: G01N33/558 ,  G01N33/549 ,  G01N2333/11 ,  G01N2333/135 ,  G01N2333/165 ,  G01N2333/4737 ,  G01N2469/20 ,  G01N33/54389 ,  G01N33/56911 ,  G01N33/56983

Abstract: A multi-tiered, diagnostic strategy incorporating: a rapid host immune response assay as an initial screening test; a rapid immunoglobulin M, immunoglobulin G and/or immunoglobulin A test; and molecular confirmatory testing, for example via real-time reverse transcription polymerase chain reaction is used for confirmation of SARS-CoV-2. The rapid host immune response assay provides information for early screening and triage of patients with symptoms. The rapid immunoglobulin testing is used to assess which patients would benefit from quarantine or further testing and provide information on population exposure/herd immunity and determines if the patient's body is responding to a viral or bacterial infection. The strategy provides both a comprehensive screening and diagnostic testing strategy. The ability to triage patients within minutes rather than hours or days is a critical component to pandemic response.

公开(公告)号：WO2017070419A1

公开(公告)日：2017-04-27

申请号：PCT/US2016/058025

申请日：2016-10-21

Applicant: RAPID PATHOGEN SCREENING, INC.

Inventor： SAMBURSKY, Robert P.

IPC: C12Q1/04 ,  G01N33/569 ,  G01N33/68

CPC classification number: C12Q1/689 ,  C12Q1/70 ,  G01N33/56911 ,  G01N33/56983 ,  G01N2333/47 ,  G01N2333/4737 ,  G01N2333/585

Abstract: Detecting a presence of MxA in combination with either the bacterial biomarker C- reactive protein or the bacterial biomarker procalcitonin increases the specificity of the bacterial biomarker with a concurrent improvement in sensitivity.

Abstract translation: 检测MxA与细菌生物标志物C反应蛋白或细菌生物标志物降钙素原组合的存在增加了细菌生物标志物的特异性，同时提高了灵敏度。

公开(公告)号：WO2011069031A3

公开(公告)日：2012-04-19

申请号：PCT/US2010058827

申请日：2010-12-03

Applicant: RAPID PATHOGEN SCREENING INC ,  SAMBURSKY ROBERT P ,  BABU UMA MAHESH ,  VANDINE ROBERT W ,  KANAUJIA GANGA V ,  ORSINI THOMAS

Inventor： SAMBURSKY ROBERT P ,  BABU UMA MAHESH ,  VANDINE ROBERT W ,  KANAUJIA GANGA V ,  ORSINI THOMAS

IPC: G01N33/538 ,  G01N33/531

CPC classification number: G01N33/558 ,  C12Q1/6825 ,  G01N33/54386 ,  C12Q2565/625 ,  C12Q2565/629

Abstract: A sample compressor applies pressure to a sample collector and a sample application zone of a test strip to transfer a sample from the sample collector and a binding partner of an analyte to the sample application zone in a lateral flow device. At least one of the binding partners of the analyte is not located on the test strip prior to use of the lateral flow device. The test strip may be a universal test strip with no molecule that specifically binds the analyte is located on the test strip. The sample compressor may be a universal sample compressor also with no molecule that specifically binds the analyte on the sample compressor. The lateral flow device may also include one or more enhancement elements, where the enhancement elements bind to the analyte sandwich to increase a detection signal in the test zone.

Abstract translation: 样品压缩机向样品收集器和测试条的样品施加区域施加压力，以将样品从样品收集器和分析物的结合配对物转移到侧向流动装置中的样品施加区域。 在使用侧流装置之前，分析物的至少一个结合配偶体不位于测试条上。 测试条可以是通用测试条，没有特异性结合分析物位于测试条上的分子。 样品压缩机可以是通用样品压缩机，也不具有特异性结合样品压缩机上的分析物的分子。 侧向流动装置还可以包括一个或多个增强元件，其中增强元件结合到分析物夹心物以增加测试区域中的检测信号。

公开(公告)号：WO2011069029A3

公开(公告)日：2011-10-13

申请号：PCT/US2010058822

申请日：2010-12-03

Applicant: RAPID PATHOGEN SCREENING INC ,  BABU UMA MAHESH ,  SAMBURSKY ROBERT P ,  VANDINE ROBERT W

Inventor： BABU UMA MAHESH ,  SAMBURSKY ROBERT P ,  VANDINE ROBERT W

IPC: C12Q1/68 ,  G01N33/50

CPC classification number: G01N33/558 ,  C12Q1/6825 ,  G01N33/54386 ,  C12Q2565/625 ,  C12Q2565/629

Abstract: Assays and methods including mobile tagged single stranded nucleic acid reagents pre-loaded on an analysis device, which are preferably tagged, but not labeled and are complementary to a strand (preferably the anti-sense strand in double stranded DNA targets) of the target nucleic acid. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. In addition, the analysis device includes a detection zone including one or more test zones that have an immobilized tag that binds to the tag on the mobile nucleic acid reagent.

Abstract translation: 包括预加载在分析装置上的移动标记的单链核酸试剂的测定和方法，所述试剂优选标记但不标记且与靶核酸的链（优选双链DNA靶标中的反义链）互补 酸。 该测定还包括运行缓冲液，其包含非特异性结合双链核酸的染料或其他可检测标记。 此外，分析装置包括检测区域，该检测区域包括具有与移动核酸试剂上的标签结合的固定标签的一个或多个测试区域。

公开(公告)号：WO2011069029A2

公开(公告)日：2011-06-09

申请号：PCT/US2010/058822

申请日：2010-12-03

Applicant: RAPID PATHOGEN SCREENING, INC. ,  BABU, Uma, Mahesh ,  SAMBURSKY, Robert, P ,  VANDINE, Robert, W.

Inventor： BABU, Uma, Mahesh ,  SAMBURSKY, Robert, P ,  VANDINE, Robert, W.

IPC: C12Q1/68 ,  G01N33/50

CPC classification number: G01N33/558 ,  C12Q1/6825 ,  G01N33/54386 ,  Y02A50/51 ,  Y02A50/53 ,  Y02A50/54 ,  Y02A50/60 ,  C12Q2565/625 ,  C12Q2565/629

Abstract: Assays and methods including mobile tagged single stranded nucleic acid reagents pre-loaded on an analysis device, which are preferably tagged, but not labeled and are complementary to a strand (preferably the anti-sense strand in double stranded DNA targets) of the target nucleic acid. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. In addition, the analysis device includes a detection zone including one or more test zones that have an immobilized tag that binds to the tag on the mobile nucleic acid reagent.

Abstract translation: 测定和方法，包括预先加载在分析装置上的移动标记单链核酸试剂，其优选地被标记但未标记并与靶核酸的链（优选双链DNA靶的反义链）互补 酸。 该测定还包括运行缓冲液，其包括仅与双链核酸非特异性结合的染料或其它可检测标记。 此外，分析装置包括检测区域，该检测区域包括具有与移动核酸试剂上的标签结合的固定化标签的一个或多个测试区域。

公开(公告)号：WO2016060916A1

公开(公告)日：2016-04-21

申请号：PCT/US2015/054582

申请日：2015-10-08

Applicant: RAPID PATHOGEN SCREENING, INC.

Inventor： BABU, Uma Mahesh ,  VANDINE, Robert W. ,  SAMBURSKY, Robert P.

IPC: A61K38/17

CPC classification number: A61K38/1709 ,  A61F9/00836 ,  A61K9/0017 ,  A61K31/20 ,  A61K31/56 ,  A61K31/573 ,  A61K31/65 ,  A61K38/13 ,  A61K45/06 ,  A61K47/02 ,  A61K47/06 ,  A61K47/10 ,  A61K47/38

Abstract: Histatins are used to treat ocular surface diseases such as dry eye. For example, histatins are included in eye drops, eye gels, ointment, glue, or embedded in (polymer) contact lenses. In some embodiments, peptide fragments from at least two different histatins are used. In some other embodiments, an additional therapeutic (nonhistatin) agent is used in combination with the histatins. In some embodiments, histatin 5 or fragments of histatin 5 are used in combination with an additional therapeutic (nonhistatin) agent. Histatins may alternatively be included as preservatives in ophthalmic preparations.

Abstract translation: 组织蛋白酶用于治疗眼睛表面疾病如干眼症。 例如，组氨酸素包括在滴眼剂，眼凝胶，软膏，胶水或嵌入（聚合物）隐形眼镜中。 在一些实施方案中，使用来自至少两种不同组氨酸的肽片段。 在一些其它实施方案中，另外的治疗剂（非组胺药）与组氨素组合使用。 在一些实施方案中，组氨素5或组氨素5的片段与另外的治疗剂（非组胺药）组合使用。 可替代地，组氨酸可作为防腐剂包含在眼用制剂中。

公开(公告)号：WO2013191906A1

公开(公告)日：2013-12-27

申请号：PCT/US2013/044244

申请日：2013-06-05

Applicant: RAPID PATHOGEN SCREENING, INC.

Inventor： VANDINE, Robert W. ,  SAMBURSKY, Robert P. ,  BABU, Uma Mahesh ,  NAGATA, Kyosuke

IPC: C12N15/63 ,  C12N15/12 ,  C12Q1/68 ,  C12Q1/00

CPC classification number: A61K38/46 ,  C12N9/14 ,  C12N9/16 ,  C12Y301/04 ,  C12Y306/05 ,  G01N33/56983

Abstract: Full length MxA constructs and truncated MxA constructs produce human MxA protein in E. coli. The full length MxA and truncated MxA constructs are preferably E. coli codon-optimized to optimize the amount of protein made using the constructs. T5 or T7 promoters can each be used in combination with either the full length MxA or the truncated MxA constructs. In one preferred embodiment, the MxA protein produced by the full length MxA or truncated MxA constructs is used in a control prep or external control. In other preferred embodiments, the MxA protein is used as a therapeutic.

Abstract translation: 全长MxA构建体和截短的MxA构建体在大肠杆菌中产生人MxA蛋白。 全长MxA和截短的MxA构建体优选是经密码子优化的E.coli，以优化使用构建体制备的蛋白质的量。 T5或T7启动子各自可以与全长MxA或截短的MxA构建体组合使用。 在一个优选实施方案中，由全长MxA或截短的MxA构建体产生的MxA蛋白用于对照制备或外部对照。 在其它优选的实施方案中，MxA蛋白用作治疗剂。

公开(公告)号：WO2010033963A3

公开(公告)日：2010-06-24

申请号：PCT/US2009057775

申请日：2009-09-22

Applicant: RAPID PATHOGEN SCREENING INC ,  SAMBURSKY ROBERT P ,  BABU UMA MAHESH ,  VANDINE ROBERT W

Inventor： SAMBURSKY ROBERT P ,  BABU UMA MAHESH ,  VANDINE ROBERT W

IPC: G01N33/569 ,  C12Q1/04

CPC classification number: G01N33/558

Abstract: A lateral flow assay detects and differentiates between viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In one preferred embodiment, the bacterial marker is CRP. In another preferred embodiment, the viral marker is MxA. In some embodiments, it is unnecessary to lyse the cells in the sample prior to applying it to the device.

Abstract translation: 侧流测定检测并区分病毒和细菌感染。 结合的护理点诊断设备测试病毒感染的标记物和细菌感染的标记物，以有效协助病毒和细菌感染的快速分化。 在一个优选的实施方案中，细菌标志物是CRP。 在另一个优选实施方案中，病毒标记是MxA。 在一些实施方案中，在将其应用于装置之前不需要溶解样品中的细胞。

公开(公告)号：WO2010009203A3

公开(公告)日：2010-04-08

申请号：PCT/US2009050645

申请日：2009-07-15

Applicant: RAPID PATHOGEN SCREENING INC ,  SAMBURSKY ROBERT P ,  BABU UMA MAHESH ,  VANDINE ROBERT W

Inventor： SAMBURSKY ROBERT P ,  BABU UMA MAHESH ,  VANDINE ROBERT W

IPC: C12Q1/68 ,  C07H21/00 ,  C12N15/11

CPC classification number: G01N33/558 ,  C12Q1/6834 ,  C12Q2565/137

Abstract: Point-of-care binding assays include at least one target nucleic acid binding in a multiplex structure with at least one sequence in a partner nucleic acid associated with a label, due to complementary base pairings between at least one sequence in the target nucleic acid and at least one sequence in the partner nucleic acid. The assays overcome the inherent deficiencies of antibody-protein antigen assays. In a preferred embodiment, color tagged nucleic acid sequences are used to bind a complementary target nucleic acid. The tagged nucleic acid sequences are preferably made from deoxyribonucleotides, ribonucleotides, or peptide nucleotides.

Abstract translation: 由于靶核酸中的至少一个序列与靶标核酸之间的互补碱基配对，所以护理点结合测定法包括至少一个靶标核酸以多重结构与靶标相关的伴侣核酸中的至少一个序列结合 在配偶体核酸中至少有一个序列。 该测定法克服了抗体 - 蛋白质抗原测定的固有缺陷。 在一个优选的实施方案中，使用彩色标记的核酸序列结合互补的靶核酸。 标记的核酸序列优选由脱氧核糖核苷酸，核糖核苷酸或肽核苷酸制成。

公开(公告)号：WO2009152209A2

公开(公告)日：2009-12-17

申请号：PCT/US2009/046848

申请日：2009-06-10

Applicant: RAPID PATHOGEN SCREENING, INC. ,  BABU, Uma, Mahesh ,  VANDINE, Robert, W. ,  SAMBURSKY, Robert, P. ,  KANAUJIA, Ganga ,  RIVAS, Richard, Jr.

Inventor： BABU, Uma, Mahesh ,  VANDINE, Robert, W. ,  SAMBURSKY, Robert, P. ,  KANAUJIA, Ganga ,  RIVAS, Richard, Jr.

IPC: G01N33/533 ,  G01N33/543

CPC classification number: G01N33/558

Abstract: The sensitivity of visually read lateral flow immunoassay tests is enhanced by adding a small quantity of fluorescing dye or fluorescing latex bead conjugates to the initial conjugate material. When the visible spectrum test line is visibly present, the test result is observed and recorded. However, in the case where the result is indeterminate, a light of an appropriate spectrum, such as a UV, visible, or infrared spectrum, is cast on the test line to excite and fluoresce the fluorescing latex beads which are bound in the test line in true positive tests to enhance the visible color at the test line.

Abstract translation: 通过向初始缀合物材料中加入少量荧光染料或荧光胶乳珠缀合物，增强了视觉上的侧向流动免疫测定的灵敏度。 当可见光谱测试线可见时，观察并记录测试结果。 然而，在结果不确定的情况下，在测试线上投射诸如UV，可见或红外光谱的适当光谱的光，以激发和发出在测试线中结合的荧光胶乳珠 在真正的阳性测试中增强测试线上的可见颜色。