公开(公告)号：WO2010096562A2

公开(公告)日：2010-08-26

申请号：PCT/US2010/024592

申请日：2010-02-18

Applicant: MASCOMA CORPORATION ,  MCBRIDE, John ,  MELLON, Mark ,  RAJGHARIA, Vineet ,  BREVNOVA, Elena, E. ,  WISWALL, Erin ,  HOGSETT, David, A. ,  LAGRANGE, Danie ,  ROSE, Shaunita ,  VAN ZYL, Emile

Inventor： MCBRIDE, John ,  MELLON, Mark ,  RAJGHARIA, Vineet ,  BREVNOVA, Elena, E. ,  WISWALL, Erin ,  HOGSETT, David, A. ,  LAGRANGE, Danie ,  ROSE, Shaunita ,  VAN ZYL, Emile

IPC: C12P7/10

CPC classification number: C12P7/10 ,  C07K14/33 ,  C07K2319/035 ,  C07K2319/70 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N15/81 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01037 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02P20/52

Abstract: The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

Abstract translation: 本发明涉及在微生物中异源纤维素酶体的工程和表达，以促进生物质转化为有用的产物。 在一些实施方案中，本发明涉及形成纤维素酶的核的骨架蛋白的表达。 纤维素酶或其他生物降解酶可以通过泊松特蛋白结构域 - 凝聚素结构域相互作用而非共价连接至支架蛋白。

公开(公告)号：WO2010060056A3

公开(公告)日：2010-07-15

申请号：PCT/US2009065571

申请日：2009-11-23

Applicant: MASCOMA CORP ,  MCBRIDE JOHN ,  BREVNOVA ELENA ,  GHANDI CHHAYAL ,  MELLON MARK ,  FROEHLICH ALAN ,  DELEAULT KRISTEN ,  RAJGHARIA VINEET ,  FLATT JIM ,  VAN ZYL EMILE ,  DEN HAAN RIAAN ,  LAGRANGE DANIE ,  ROSE SHAUNITA ,  PENTTILA MERJA ,  ILMEN MARJA ,  SIIKA-AHO MATTI ,  UUSITALO JAANA ,  HAU HEIDI HANSON ,  RICE CHARLES ,  VILLARI JEFF ,  STONEHOUSE EMILY A ,  GILBERT ALAN ,  KEATING JEFFREY D ,  XU HAOWEN ,  WILLES DEIDRE ,  SHIKHARE INDRANEEL ,  THORNGREN NAOMI ,  WARNER ANNE K ,  MURPHY DAN

Inventor： MCBRIDE JOHN ,  BREVNOVA ELENA ,  GHANDI CHHAYAL ,  MELLON MARK ,  FROEHLICH ALAN ,  DELEAULT KRISTEN ,  RAJGHARIA VINEET ,  FLATT JIM ,  VAN ZYL EMILE ,  DEN HAAN RIAAN ,  LAGRANGE DANIE ,  ROSE SHAUNITA ,  PENTTILA MERJA ,  ILMEN MARJA ,  SIIKA-AHO MATTI ,  UUSITALO JAANA ,  HAU HEIDI HANSON ,  RICE CHARLES ,  VILLARI JEFF ,  STONEHOUSE EMILY A ,  GILBERT ALAN ,  KEATING JEFFREY D ,  XU HAOWEN ,  WILLES DEIDRE ,  SHIKHARE INDRANEEL ,  THORNGREN NAOMI ,  WARNER ANNE K ,  MURPHY DAN

IPC: C12N15/09 ,  C12N15/81 ,  C12P21/00

CPC classification number: C12P7/10 ,  C12N9/2437 ,  C12N9/2445 ,  C12N15/80 ,  C12P7/14 ,  C12P39/00 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

Abstract: Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes

Abstract translation: 包含表达异源纤维素酶的Kluveryomyces的宿主细胞从纤维素产生乙醇此外，表达不同杂种纤维素酶的多个宿主细胞可以共同共培养并用于从纤维素生产乙醇可以使用酵母菌株的重组酵母菌株和共培养物 自己生产乙醇，也可以与外部添加的纤维素酶组合使用，以提高发酵和发酵过程的效率

公开(公告)号：WO2010060056A2

公开(公告)日：2010-05-27

申请号：PCT/US2009/065571

申请日：2009-11-23

Applicant: MASCOMA CORPORATION ,  MCBRIDE, John ,  BREVNOVA, Elena ,  GHANDI, Chhayal ,  MELLON, Mark ,  FROEHLICH, Alan ,  DELEAULT, Kristen ,  RAJGHARIA, Vineet ,  FLATT, Jim ,  VAN ZYL, Emile ,  DEN HAAN, Riaan ,  LAGRANGE, Danie ,  ROSE, Shaunita ,  PENTTILA, Merja ,  ILMEN, Marja ,  SIIKA-AHO, Matti ,  UUSITALO, Jaana ,  HAU, Heidi Hanson ,  RICE, Charles ,  VILLARI, Jeff ,  STONEHOUSE, Emily A. ,  GILBERT, Alan ,  KEATING, Jeffrey D. ,  XU, Haowen ,  WILLES, Deidre ,  SHIKHARE, Indraneel ,  THORNGREN, Naomi ,  WARNER, Anne K. ,  MURPHY, Dan

Inventor： MCBRIDE, John ,  BREVNOVA, Elena ,  GHANDI, Chhayal ,  MELLON, Mark ,  FROEHLICH, Alan ,  DELEAULT, Kristen ,  RAJGHARIA, Vineet ,  FLATT, Jim ,  VAN ZYL, Emile ,  DEN HAAN, Riaan ,  LAGRANGE, Danie ,  ROSE, Shaunita ,  PENTTILA, Merja ,  ILMEN, Marja ,  SIIKA-AHO, Matti ,  UUSITALO, Jaana ,  HAU, Heidi Hanson ,  RICE, Charles ,  VILLARI, Jeff ,  STONEHOUSE, Emily A. ,  GILBERT, Alan ,  KEATING, Jeffrey D. ,  XU, Haowen ,  WILLES, Deidre ,  SHIKHARE, Indraneel ,  THORNGREN, Naomi ,  WARNER, Anne K. ,  MURPHY, Dan

IPC: C12P7/06

CPC classification number: C12P7/10 ,  C12N9/2437 ,  C12N9/2445 ,  C12N15/80 ,  C12P7/14 ,  C12P39/00 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthemore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Abstract translation: 本发明涉及纤维素分解宿主细胞。 表达异源纤维素酶的本发明的宿主细胞能够从纤维素中产生乙醇。 根据本发明，表达异源纤维素酶组合的宿主细胞可用于从纤维素生产乙醇。 此外，表达不同杂种纤维素酶的多个宿主细胞可以共同共培养并用于从纤维素生产乙醇。 此外，本发明首次证明了Kluveryomyces从纤维素中生产乙醇的能力。 本发明的酵母菌株的酵母菌株和共培养物可以独立地用于生产乙醇，也可以与外部添加的纤维素酶组合使用，以提高糖化和发酵过程的效率。

公开(公告)号：WO2008155665A2

公开(公告)日：2008-12-24

申请号：PCT/IB2008002398

申请日：2008-05-09

Applicant: UNIV STELLENBOSCH ,  VAN ZYL WILLEM HEBER ,  VAN ROOYEN RONEL ,  ROSE SHAUNITA HELLOUISE

Inventor： VAN ZYL WILLEM HEBER ,  VAN ROOYEN RONEL ,  ROSE SHAUNITA HELLOUISE

CPC classification number: C12P7/10 ,  C12N1/36 ,  C12N9/2445 ,  C12Y302/01021 ,  Y02E50/16 ,  Y02P20/52

Abstract: The present invention relates to methods for improving a host cell's ability to utilize the disaccharide cellobiose. In some embodiments, a transformed cell expresses intracellular ß- glucosidase. In other embodiments, a transformed host cell is able to grow on media wherein cellobiose is the sole carbon source. In other embodiments, selection methods are provided which improve a host cell's ability to grow on cellobiose-containing media.

Abstract translation: 本发明涉及提高宿主细胞利用二糖纤维二糖的能力的方法。 在一些实施方案中，转化的细胞表达细胞内β-葡糖苷酶。 在其它实施方案中，转化的宿主细胞能够在纤维二糖是唯一碳源的培养基上生长。 在其它实施方案中，提供了提高宿主细胞在含纤维二糖的培养基上生长能力的选择方法。

公开(公告)号：WO2010096562A3

公开(公告)日：2010-10-14

申请号：PCT/US2010024592

申请日：2010-02-18

Applicant: MASCOMA CORP ,  MCBRIDE JOHN ,  MELLON MARK ,  RAJGHARIA VINEET ,  BREVNOVA ELENA E ,  WISWALL ERIN ,  HOGSETT DAVID A ,  LAGRANGE DANIE ,  ROSE SHAUNITA ,  VAN ZYL EMILE

Inventor： MCBRIDE JOHN ,  MELLON MARK ,  RAJGHARIA VINEET ,  BREVNOVA ELENA E ,  WISWALL ERIN ,  HOGSETT DAVID A ,  LAGRANGE DANIE ,  ROSE SHAUNITA ,  VAN ZYL EMILE

IPC: C12P7/10 ,  C07K14/33 ,  C12N9/42 ,  C12N15/56 ,  C12N15/62

CPC classification number: C12P7/10 ,  C07K14/33 ,  C07K2319/035 ,  C07K2319/70 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N15/81 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01037 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02P20/52

Abstract: The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

Abstract translation: 本发明涉及在微生物中工程化和表达异源纤维素体以促进生物质向有用产物的转化。 在一些实施方案中，本发明涉及形成细胞体的细胞核的支架蛋白的表达。 凭借dockerin结构域 - 粘连素结构域相互作用，纤维素酶或其他生物质降解酶可以非共价连接到支架蛋白。

公开(公告)号：WO2008155665A3

公开(公告)日：2008-12-24

申请号：PCT/IB2008/002398

申请日：2008-05-09

Applicant: UNIVERSITY OF STELLENBOSCH ,  VAN ZYL, Willem, Heber ,  VAN ROOYEN, Ronel ,  ROSE, Shaunita, Hellouise

Inventor： VAN ZYL, Willem, Heber ,  VAN ROOYEN, Ronel ,  ROSE, Shaunita, Hellouise

IPC: C12N9/42 ,  C12N1/15 ,  C12P7/10 ,  C12N1/36

Abstract: The present invention relates to methods for improving a host cell's ability to utilize the disaccharide cellobiose. In some embodiments, a transformed cell expresses intracellular β- glucosidase. In other embodiments, a transformed host cell is able to grow on media wherein cellobiose is the sole carbon source. In other embodiments, selection methods are provided which improve a host cell's ability to grow on cellobiose-containing media.