公开(公告)号：WO2008118416A1

公开(公告)日：2008-10-02

申请号：PCT/US2008/003863

申请日：2008-03-25

Applicant: ROTH, Jonathan, N.

Inventor： ROTH, Jonathan, N.

IPC: C12N5/00

CPC classification number: C12N1/20

Abstract: The method provides a simple, accurate and inexpensive way to prepare culture media for conducting microbiological analysis with an absolute minimum of facilities, extraneous equipment and time consuming procedures. The invention introduces the use of a carrier piece that is suitable for carrying a reactant material for combining with growth media. The carrier piece may be formed using (non-)porous, (non-)absorbent material, including paper, plastic, gum, fabric, or acetate. The carrier piece carries a single one or a combination of various reactant materials that are released into the medium or medium/test sample mixture upon contact therewith. The reactant materials may include, among other things, nutrients, inhibitors, including antibiotics and bile salts, enzyme substrates, and/or a catalyst for a gelling agent contained in the growth medium. The growth medium, the carrier piece with the appropriate reactant material(s), and the test sample can be easily packaged and sold separately or as a kit and used anywhere.

Abstract translation: 该方法提供了一种简单，准确和便宜的方法，以绝对最小的设施，无关的设备和耗时的程序来制备用于进行微生物分析的培养基。 本发明介绍了适用于携带用于与生长介质组合的反应物材料的载体片的用途。 载体片可以使用（非）多孔（非）吸收材料，包括纸，塑料，树胶，织物或乙酸酯形成。 载体片承载与其接触时释放到介质或介质/测试样品混合物中的单一的或各种反应物的组合。 反应物料可包括营养物质，抑制剂，包括抗生素和胆汁盐，酶底物和/或包含在生长培养基中的胶凝剂的催化剂。 生长介质，具有适当反应物质的载体片和测试样品可以容易地单独或作为试剂盒包装和销售并在任何地方使用。

公开(公告)号：WO2006107583A1

公开(公告)日：2006-10-12

申请号：PCT/US2006/010177

申请日：2006-03-21

Applicant: MICROLOGY LABORATORIES, LLC ,  ROTH, Geoffrey, N. ,  ROTH, Jonathan, N.

Inventor： ROTH, Geoffrey, N. ,  ROTH, Jonathan, N.

IPC: C12Q1/04

CPC classification number: C12Q1/04 ,  C12Q1/045 ,  C12Q1/10 ,  C12Q2334/00 ,  C12Q2334/50 ,  G01N2333/245 ,  G01N2333/25 ,  G01N2333/255 ,  G01N2333/924 ,  Y02A50/451

Abstract: A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella in a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium. In one embodiment, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red- pink color, and Salmonella colonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.

Abstract translation: 用于在测试样品中检测，定量，鉴定和区分多达四（4）种分离的生物材料的测试介质和方法。 公开了一种测试介质，其允许在单个测试介质中产生特定酶的生物实体的环境光聚集体（其可以包括一般大肠杆菌，大肠杆菌，气单胞菌属和沙门氏菌）进行定量和鉴别。 公开了一类新的非致冷底物，其产生基本上黑色的非扩散性沉淀物。 该沉淀物不受来自测试介质中存在的其它显色底物的干扰。 在一个实施方案中，选择底物使得存在于测试培养基中的大肠杆菌菌落显示为基本上黑色，一般大肠菌群在测试培养基中显示为蓝紫色，测试培养基中存在的气单胞菌菌落显示为一般 红色粉红色和沙门氏菌菌落呈普通绿绿色。 还公开了用于其检测和定量的其它微生物和颜色可能性。 公开了一种制备包含抑制剂的测试介质的抑制剂和方法。

公开(公告)号：WO2008118416A8

公开(公告)日：2010-09-16

申请号：PCT/US2008003863

申请日：2008-03-25

Applicant: ROTH JONATHAN N ,  ROTH GEOFFREY N

Inventor： ROTH JONATHAN N ,  ROTH GEOFFREY N

IPC: C12N5/00

CPC classification number: C12N1/20

Abstract: The method provides a simple, accurate and inexpensive way to prepare culture media for conducting microbiological analysis with an absolute minimum of facilities, extraneous equipment and time consuming procedures. The invention introduces the use of a carrier piece that is suitable for carrying a reactant material for combining with growth media. The carrier piece may be formed using (non-)porous, (non-)absorbent material, including paper, plastic, gum, fabric, or acetate. The carrier piece carries a single one or a combination of various reactant materials that are released into the medium or medium/test sample mixture upon contact therewith. The reactant materials may include, among other things, nutrients, inhibitors, including antibiotics and bile salts, enzyme substrates, and/or a catalyst for a gelling agent contained in the growth medium. The growth medium, the carrier piece with the appropriate reactant material(s), and the test sample can be easily packaged and sold separately or as a kit and used anywhere.

Abstract translation: 该方法提供了一种简单，准确和便宜的方法，以绝对最小的设施，无关的设备和耗时的程序来制备用于进行微生物分析的培养基。 本发明介绍了适用于携带用于与生长介质组合的反应物材料的载体片的用途。 载体片可以使用（非）多孔（非）吸收材料，包括纸，塑料，树胶，织物或乙酸酯形成。 载体片承载与其接触时释放到介质或介质/测试样品混合物中的单一的或各种反应物的组合。 反应物料可包括营养物质，抑制剂，包括抗生素和胆汁盐，酶底物和/或包含在生长培养基中的胶凝剂的催化剂。 生长介质，具有适当反应物质的载体片和测试样品可以容易地单独或作为试剂盒包装和销售并在任何地方使用。

公开(公告)号：WO2013006148A1

公开(公告)日：2013-01-10

申请号：PCT/US2011/001192

申请日：2011-07-07

Applicant: ROTH, Geoffrey, N. ,  ROTH, Jonathan, N.

Inventor： ROTH, Geoffrey, N. ,  ROTH, Jonathan, N.

IPC: G01N33/52 ,  C12Q1/04 ,  C12Q1/25 ,  G01N33/58

CPC classification number: C12Q1/04 ,  C12Q2334/50 ,  G01N33/52 ,  G01N33/582 ,  G01N33/583

Abstract: A method of detection of cells, microorganisms, or molecules by the use of various combinations of fluorogens and a chromogens which yield fluorophores and chromophores when cleaved by specific enzymes and which can be viewed by UV and visible light. Included is the method of application of a family of compounds producing both insoluble fluorophores and chromophores identified as dual enzyme substrates.

Abstract translation: 通过使用氟化物和发色团的各种组合检测细胞，微生物或分子的方法，当由特异性酶切割时产生荧光团和发色团，并且可以通过紫外线和可见光观察。 包括产生两种不溶性荧光团和鉴定为双酶底物的发色团的化合物家族的应用方法。