公开(公告)号：WO2013006148A1

公开(公告)日：2013-01-10

申请号：PCT/US2011/001192

申请日：2011-07-07

Applicant: ROTH, Geoffrey, N. ,  ROTH, Jonathan, N.

Inventor： ROTH, Geoffrey, N. ,  ROTH, Jonathan, N.

IPC: G01N33/52 ,  C12Q1/04 ,  C12Q1/25 ,  G01N33/58

CPC classification number: C12Q1/04 ,  C12Q2334/50 ,  G01N33/52 ,  G01N33/582 ,  G01N33/583

Abstract: A method of detection of cells, microorganisms, or molecules by the use of various combinations of fluorogens and a chromogens which yield fluorophores and chromophores when cleaved by specific enzymes and which can be viewed by UV and visible light. Included is the method of application of a family of compounds producing both insoluble fluorophores and chromophores identified as dual enzyme substrates.

Abstract translation: 通过使用氟化物和发色团的各种组合检测细胞，微生物或分子的方法，当由特异性酶切割时产生荧光团和发色团，并且可以通过紫外线和可见光观察。 包括产生两种不溶性荧光团和鉴定为双酶底物的发色团的化合物家族的应用方法。

公开(公告)号：WO02000922A1

公开(公告)日：2002-01-03

申请号：PCT/FR2001/002033

申请日：2001-06-27

IPC: C12Q1/04

CPC classification number: C12Q1/04 ,  C12Q2334/50 ,  G01N2333/28 ,  G01N2333/924 ,  Y02A50/52

Abstract: The invention concerns a culture medium for isolating Vibrio bacteria, characterised in that it comprises, in a Vibrio culture medium, at least a chromogenic agent selected among the beta -glucosidase substrates and the beta -galactosidase substrates.

Abstract translation: 本发明涉及一种用于分离弧菌细菌的培养基，其特征在于，其在维管培养基中包含至少一种选自β-葡糖苷酶底物和 β-半乳糖苷酶底物。

公开(公告)号：WO1997036001A1

公开(公告)日：1997-10-02

申请号：PCT/US1997004671

申请日：1997-03-21

Applicant: RCR SCIENTIFIC, INC.

Inventor： RCR SCIENTIFIC, INC. ,  ROTH, Jonathan, N. ,  BONTRAGER, Gordon, L.

IPC: C12Q01/04

CPC classification number: C12Q1/04 ,  C12Q1/10 ,  C12Q2334/50 ,  C12Q2334/52 ,  G01N2333/24 ,  Y10S435/849 ,  Y10S435/879

Abstract: A test method and medium for quantitatively identifying and differentiating biological materials in a test sample containing a plurality of different biological materials. A first biological material in the sample has enzyme specificity for a first chromogenic substrate, a second biological material has enzyme specificity for a second chromogenic substrate, and a third biological material has enzyme specificity for one of the chromogenic substrates. The chromogenic substrates form respective first and second colored water insoluble compounds upon reaction with specific enzymes from the biological materials. The first and second biological materials are capable of fermenting a sugar, and the third biological material does not ferment the sugar. The test medium is adjusted to a pH conducive for color change of a pH indicator upon acidification of a portion of the test medium due to fermentation of the sugar, which color change results in the formation of a colored zone around the water insoluble compounds of the sugar-fermenting biological materials, the colored zone being distinguishable from the colors of the water insoluble compounds.

Abstract translation: 一种用于在含有多种不同生物材料的测试样品中定量鉴定和分化生物材料的测试方法和介质。 样品中的第一生物材料对于第一显色底物具有酶特异性，第二生物材料对于第二显色底物具有酶特异性，并且第三生物材料对于其中一个显色底物具有酶特异性。 当与生物材料的特定酶反应时，显色底物形成相应的第一和第二有色水不溶性化合物。 第一和第二生物材料能够发酵糖，第三生物材料不发酵糖。 将测试培养基调节至pH，由于糖的发酵而使一部分测试培养基酸化，导致pH值指示剂的颜色变化，这种颜色变化导致在水不溶性化合物周围形成着色区 糖发酵生物材料，着色区域与水不溶性化合物的颜色不同。

公开(公告)号：WO1995020674A1

公开(公告)日：1995-08-03

申请号：PCT/US1995000778

申请日：1995-01-20

Applicant: MINNESOTA MINING AND MANUFACTURING COMPANY

Inventor： MINNESOTA MINING AND MANUFACTURING COMPANY ,  LUND, Marlys, E.

IPC: C12Q01/04

CPC classification number: C12Q1/04 ,  C12M25/06 ,  C12M41/36 ,  C12Q1/14 ,  C12Q2334/50 ,  C12Q2334/52 ,  G01N2333/31 ,  G01N2333/315 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/882

Abstract: The present invention exploits the herein first reported, empirical observation that even though staphylococci produce the enzyme, beta -glucosidase, this genus of bacteria is not able to produce a metabolite that will enzymatically react with 5-bromo-4-chloro-3-indolyl- beta -D-glucopyranoside, a substrate commonly used to detect the presence of beta -glucosidase produced by other bacteria. In view of this unexpected observation, one embodiment of the present invention includes a selective medium containing inhibitors to enhance staphylococci growth as well as a first glucopyranoside substrate, such as 5-bromo-4-chloro-3-indolyl- beta -D-glucopyranoside, and a second phosphatase substrate, such as 6-chloro-3-indolylphosphate or 5-bromo-6-chloro-3-indolylphosphate. In this embodiment, staphylococci in a sample will produce metabolites that will react with the phosphate substrate in the medium to produce colonies having red to red-violet color while other bacteria in the sample will produce beta -glucosidase that will react with the glucopyranosidase substrate in the medium and produce colonies having a blue color. In a particularly preferred embodiment of this invention, a thin film culture plate device, such as a PETRIFILM culture plate device, is prepared using a dry culture medium containing selected inhibitors, a first glucopyranoside substrate and a second phosphate substrate. When the thin film culture plate device is inoculated with a sample and then incubated for a sufficient period of time, staphylococci in the sample will produce colonies on the thin film device having a red color and other bacteria in the sample will produce colonies on the device having a blue color.

Abstract translation: 本发明利用了本文首先报道的经验观察，即使葡萄球菌产生酶β-葡萄糖苷酶，这种细菌不能产生将与5-溴-4-氯-3-吲哚基酶反应的代谢物 β-D-吡喃葡萄糖苷，通常用于检测由其他细菌产生的β-葡糖苷酶的存在的底物。 鉴于这种意想不到的观察，本发明的一个实施方案包括含有抑制剂以增强葡萄球菌生长的选择性培养基以及第一吡喃葡萄糖苷底物，例如5-溴-4-氯-3-吲哚基-β-D-吡喃葡萄糖苷 ，和第二磷酸酶底物，例如磷酸6-氯-3-吲哚酯或5-溴-6-氯-3-吲哚磷酸酯。 在该实施方案中，样品中的葡萄球菌将产生将在培养基中与磷酸盐底物反应的代谢物，以产生具有红色至红 - 紫色的菌落，而样品中的其他细菌将产生将与吡喃葡萄糖苷酶底物反应的β-葡糖苷酶 培养基并产生具有蓝色的菌落。 在本发明的一个特别优选的实施方案中，使用含有选择的抑制剂，第一吡喃葡萄糖苷底物和第二磷酸盐底物的干培养基，制备诸如PETRIFILM培养板装置的薄膜培养板装置。 当薄膜培养板装置接种样品然后孵育足够的时间时，样品中的葡萄球菌将在具有红色的薄膜装置上产生菌落，并且样品中的其它细菌将在装置上产生菌落 有蓝色。

公开(公告)号：WO1994008043A1

公开(公告)日：1994-04-14

申请号：PCT/FR1993000988

申请日：1993-10-06

Applicant: RAMBACH, Alain

IPC: C12Q01/04

CPC classification number: C12Q1/045 ,  C12Q1/10 ,  C12Q2334/50 ,  C12Q2334/52 ,  G01N2333/245

Abstract: The invention describes a culture medium for the enumeration of (E. coli) which comprises, in addition to a culture medium for (E. coli), a combination of a chromogenic compound derived from indolylglucuronic acid as a substrate of the GUS enzyme and a derivative of alkyl, alkenyl or aryl glucuronic acid, serving as a chromogenic agent. A method for the enumeration of (E. coli) is also disclosed.

Abstract translation: 本发明描述了一种用于计数（大肠杆菌）的培养基，除了用于（大肠杆菌）的培养基之外，还包含衍生自作为GUS酶底物的吲哚基葡萄糖醛酸的显色化合物和 作为显色剂的烷基，链烯基或芳基葡糖醛酸的衍生物。 还公开了（大肠杆菌）的计数方法。

公开(公告)号：WO2008104681A2

公开(公告)日：2008-09-04

申请号：PCT/FR2008/050185

申请日：2008-02-07

Applicant: BIOMÉRIEUX ,  MONGET, Daniel ,  ORENGA, Sylvain ,  PEYRET, Michel ,  ROGER-DALBERT, Céline

Inventor： MONGET, Daniel ,  ORENGA, Sylvain ,  PEYRET, Michel ,  ROGER-DALBERT, Céline

IPC: C12Q1/04

CPC classification number: C12Q1/045 ,  C12Q1/10 ,  C12Q1/34 ,  C12Q2334/50 ,  G01N2333/245

Abstract: L'invention concerne un procédé de détection et/ou d'identification d' E. coli dans un échantillon urinaire selon lequel a) on ensemence l'échantillon urinaire susceptible de contenir E. coli sur un milieu de détection comprenant un premier substrat choisi parmi un substrat de beta-glucuronidase, beta-galactosidase, alpha-galactosidase, d'une enzyme d'acidification du Lactose, beta-ribosidase, phosphatase, L-Alanine-aminopeptidase, L-Leucine-aminopeptidase, un deuxième substrat, différent dudit premier substrat, choisi parmi un substrat de beta-glucuronidase, beta-galactosidase, alpha-galactosidase, d'une enzyme d'acidification du Lactose, beta-ribosidase, phosphatase, L-Alanine-aminopeptidase, L-Leucine-aminopeptidase pour obtenir des colonies de bactéries; b) on identifie les colonies réagissant avec le premier substrat et/ou le deuxième substrat comme étant des colonies d' E. coli .

Abstract translation: 本发明涉及一种用于检测和/或鉴定尿液样本中的大肠杆菌的方法，其包括：将可能含有大肠杆菌的尿液样本接种在检测培养基上，所述检测培养基包含选自β-葡糖醛酸糖苷酶，β 用于酸乳化乳糖，β-核糖苷酶，磷酸酶，L-丙氨酸 - 氨基肽酶和L-白血病 - 氨基肽酶的酶，以及与第一底物不同的第二底物，并选自 β-葡糖苷酸酶，β-半乳糖苷酶和α-半乳糖苷酶底物，用于酸乳酸，β-核糖苷酶，磷酸酶，L-丙氨酸 - 氨基肽酶和L-白细胞 - 氨基肽酶的酶用于获得细菌菌落; 和b）鉴定作为大肠杆菌菌落与第一底物和/或第二底物反应的菌落。

公开(公告)号：WO2007000530A3

公开(公告)日：2007-05-18

申请号：PCT/FR2006050446

申请日：2006-05-16

Applicant: BIOMERIEUX SA ,  MONGET DANIEL ,  ROBICHON DENIS

Inventor： MONGET DANIEL ,  ROBICHON DENIS

IPC: C12Q1/04

CPC classification number: C12Q1/045 ,  C12Q2334/50 ,  G01N2333/28 ,  G01N2333/924 ,  Y02A50/451 ,  Y10S435/909

Abstract: The invention relates to a reaction medium for Vibrio group bacteria cholerae (cholerae/vulnificus and mimicus) and Vibrio parahaemolyticus comprising: a substrate enabling the detection of a ß-galactosidase enzymatic activity; a sugar, and; a colored indicator. The invention also relates to the use of this medium for isolating and identifying Vibrio group cholerae (cholerae/vulnificus and mimicus) and Vibrio parahaemol

Abstract translation: 本发明涉及用于弧菌族霍乱弧菌（霍乱弧菌/创伤和模拟物）和副溶血性弧菌的反应介质，其包含：能够检测β-半乳糖苷酶酶活性的底物; 一个糖，和; 一个彩色指示器。 本发明还涉及该培养基用于分离和鉴定霍乱弧菌群（霍乱弧菌/创伤和模拟物）和副溶血性弧菌

公开(公告)号：WO2006107583A1

公开(公告)日：2006-10-12

申请号：PCT/US2006/010177

申请日：2006-03-21

Applicant: MICROLOGY LABORATORIES, LLC ,  ROTH, Geoffrey, N. ,  ROTH, Jonathan, N.

Inventor： ROTH, Geoffrey, N. ,  ROTH, Jonathan, N.

IPC: C12Q1/04

CPC classification number: C12Q1/04 ,  C12Q1/045 ,  C12Q1/10 ,  C12Q2334/00 ,  C12Q2334/50 ,  G01N2333/245 ,  G01N2333/25 ,  G01N2333/255 ,  G01N2333/924 ,  Y02A50/451

Abstract: A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella in a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium. In one embodiment, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red- pink color, and Salmonella colonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.

Abstract translation: 用于在测试样品中检测，定量，鉴定和区分多达四（4）种分离的生物材料的测试介质和方法。 公开了一种测试介质，其允许在单个测试介质中产生特定酶的生物实体的环境光聚集体（其可以包括一般大肠杆菌，大肠杆菌，气单胞菌属和沙门氏菌）进行定量和鉴别。 公开了一类新的非致冷底物，其产生基本上黑色的非扩散性沉淀物。 该沉淀物不受来自测试介质中存在的其它显色底物的干扰。 在一个实施方案中，选择底物使得存在于测试培养基中的大肠杆菌菌落显示为基本上黑色，一般大肠菌群在测试培养基中显示为蓝紫色，测试培养基中存在的气单胞菌菌落显示为一般 红色粉红色和沙门氏菌菌落呈普通绿绿色。 还公开了用于其检测和定量的其它微生物和颜色可能性。 公开了一种制备包含抑制剂的测试介质的抑制剂和方法。

公开(公告)号：WO02053705A1

公开(公告)日：2002-07-11

申请号：PCT/FR2002/000024

申请日：2002-01-04

IPC: G01N21/77 ,  C12N1/20 ,  C12Q1/04 ,  G01N21/78

CPC classification number: C12Q1/04 ,  C12N1/20 ,  C12Q2334/22 ,  C12Q2334/50 ,  C12Q2334/52

Abstract: The invention concerns a culture medium for isolating bacteria of Listeria genus, in particular L. monocytogenes , characterised in that it comprises in a Listeria culture medium, at least a specific agent for identifying gamma -mannosidase for use in solid medium.

Abstract translation: 本发明涉及用于分离利斯特氏菌属细菌的培养基，特别是单核细胞增生李斯特氏菌，其特征在于其在利斯特氏菌培养基中包含用于鉴定用于固体培养基的γ-甘露糖苷酶的至少一种特异性试剂。

公开(公告)号：WO1996038584A1

公开(公告)日：1996-12-05

申请号：PCT/GB1996001296

申请日：1996-05-31

Applicant: CELSIS INTERNATIONAL PLC ,  GRANT, Peter, Leonard ,  FORSTER, Simon, James ,  THOMAS, Brian

Inventor： CELSIS INTERNATIONAL PLC

IPC: C12Q01/10

CPC classification number: C12Q1/045 ,  C12Q1/10 ,  C12Q2334/50 ,  C12Q2334/52

Abstract: In an assay for coliform or other microorganisms in a sample, which comprises contacting a mixture of the sample and a liquid growth medium with a material that undergoes a detectable change in the presence of the microorganisms to be assayed, the sample is contained in a well whose base is defined by a filter material, and the mixture is formed by placing the well on the growth medium. A preferred embodiment additionally comprises removing the sample and conducting a further assay adapted to distinguish between the microorganisms to be assayed and any other that may have given a false positive.

Abstract translation: 在样品中的大肠杆菌或其他微生物的测定中，其包括将样品和液体生长培养基的混合物与待测定的微生物存在下经历可检测变化的物质接触，将样品包含在孔中 其基底由过滤材料限定，并且通过将孔置于生长培养基上形成混合物。 优选的实施方案另外包括去除样品并进行适于区分要测定的微生物和可能具有假阳性的任何其它的另外的测定。