公开(公告)号：WO2011122692A1

公开(公告)日：2011-10-06

申请号：PCT/JP2011/058474

申请日：2011-03-28

Applicant: CANON KABUSHIKI KAISHA ,  NAITO, Jumpei ,  SATOH, Shuya ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

Inventor： NAITO, Jumpei ,  SATOH, Shuya ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

IPC: G01N27/62 ,  G01N27/64

CPC classification number: G01N33/6851 ,  H01J49/00 ,  Y10T436/182

Abstract: The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.

Abstract translation: 本发明要解决的问题是提供一种用于在使用带电粒子的质谱法中治疗包含半胱氨酸残基的多肽以提高其检测灵敏度的方法。 提供了包含半胱氨酸残基的多肽的质谱法，其包括用氰化剂处理包含半胱氨酸残基的多肽，用碱处理氰化多肽，然后用带电粒子对碱处理的多肽进行解吸，用于质谱法 。