公开(公告)号：WO2017035347A1

公开(公告)日：2017-03-02

申请号：PCT/US2016/048687

申请日：2016-08-25

Applicant: THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  FELDMAN, David ,  SAZER, Daniel ,  BLAINEY, Paul

Inventor： FELDMAN, David ,  SAZER, Daniel ,  BLAINEY, Paul

IPC: C12P19/34 ,  C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/6844 ,  B01L3/502761 ,  G01N21/253 ,  G01N21/6452 ,  G01N21/65 ,  C12Q2563/155 ,  C12Q2563/159 ,  C12Q2565/514

Abstract: Embodiments disclosed herein provide reagents and methods for high-throughput screening of nucleic acid sequence variations in nucleic acid containing specimens. Nucleic acid specimens to be screened are loaded into separate discrete volumes. Optically encoded particles are used to deliver primers to amplify one or more sequences comprising the nucleic acid sequence variation. The optically encoded particles may be delivered to the discrete volumes randomly resulting in a random combination of optically encoded particles in each well, or a unique combination of optically encoded particles may be specifically assigned to each discrete volume. The observable combination of optically encoded particles may then be used to identify each discrete volume.

Abstract translation: 本文公开的实施方案提供了用于高通量筛选含核酸标本中核酸序列变异的试剂和方法。 要筛选的核酸标本被加载到单独的离散体积中。 光学编码的颗粒用于递送引物以扩增包含核酸序列变异的一个或多个序列。 光学编码的颗粒可以随机地输送到离散的体积，导致每个孔中光学编码的颗粒的随机组合，或者光学编码的颗粒的独特组合可以具体地分配给每个离散的体积。 然后可以使用光学编码的颗粒的可观察组合来识别每个离散体积。