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公开(公告)号：WO2018046689A1
公开(公告)日：2018-03-15
申请号：PCT/EP2017/072637
申请日：2017-09-08
发明人： HUNG, Tran Quang , CHIN, Wai Hoe , SUN, Yi , WOLFF, Anders , BANG, Dang Duong , MATTEUCCI, Marco
IPC分类号： G01N21/64
CPC分类号： G01N21/6452 , G01N21/6428 , G01N21/6458
摘要： The invention relates to an optical chip comprising at least one, preferably a plurality of, solid optical element(s), where the optical element comprises an upper planar detection surface to which a moiety for capturing and/or detecting an analyte may be linked. The invention further provides methods of using the optical chip.
摘要翻译： 本发明涉及包括至少一个，优选多个固体光学元件的光学芯片，其中光学元件包括上部平面检测表面，用于捕获和/ 或检测分析物可能被链接。 本发明还提供了使用光学芯片的方法。
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公开(公告)号：WO2018031687A1
公开(公告)日：2018-02-15
申请号：PCT/US2017/046153
申请日：2017-08-09
CPC分类号： G01N21/6452 , G01N21/0332 , G01N21/6456 , G01N21/6458 , G01N21/6486 , G01N2021/6419 , G01N2201/0627
摘要： Various embodiments are directed to a fluorescence reader for assessing a fluorescence intensity of one or more substance samples. The fluorescence reader may comprise a sample support structure for accepting sample wells containing corresponding samples therein, an illumination structure positioned below the sample support structure and configured to illuminate one or more samples positioned within the sample wells, and an imaging structure positioned above the sample support structure and configured to detect a fluorescence emitted by the one or more samples. The illumination structure may comprise one or more reflective perimeter walls, together with the support structure, defining an enclosed illumination cavity, and one or more light sources positioned within the enclosed illumination cavity and configured to emit light causing the one or more samples to fluoresce.
摘要翻译： 各种实施方案涉及用于评估一种或多种物质样品的荧光强度的荧光读取器。 荧光读取器可以包括用于接受其中包含相应样品的样品孔的样品支撑结构，定位在样品支撑结构下方并且被配置为照射位于样品阱内的一个或多个样品的照明结构以及定位在样品支架上方的成像结构 结构并且被配置为检测由一个或多个样品发射的荧光。 照明结构可以包括与支撑结构一起限定封闭照明腔的一个或多个反射周壁以及定位在封闭照射腔内并且被配置为发射使一个或多个样本发荧光的光的一个或多个光源。
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3.发明申请MICROSCOPE SYSTEM WITH TRANSILLUMINATION-BASED AUTOFOCUSING FOR PHOTOLUMINESCENCE IMAGING 审中-公开自动聚焦荧光显微镜系统用于光致发光成像
公开(公告)号：WO2017070380A1
公开(公告)日：2017-04-27
申请号：PCT/US2016/057962
申请日：2016-10-20
CPC分类号： G02B21/244 , G01N21/6452 , G01N21/6458 , G02B7/38 , G02B21/086 , G02B21/16 , G02B21/26 , G02B21/367
摘要： Microscope system for, and methods of, imaging a sample including biological cells. In an exemplary method, light transmitted through the sample may be detected for a first set of focal positions to collect a first stack of images. Values of a focus metric may be calculated for the first stack of images. A candidate focal position may be determined based on the values. Photoluminescence may be detected from the sample for a second set of focal positions to collect a second stack of images. The second set of focal positions may define a smaller range than the first set of focal positions. At least one focal position of the second set of focal positions may be based on the candidate focal position. In other words, the candidate focal position may serve as a guide for finding a suitable photoluminescence focal position.
摘要翻译： 对含有生物细胞的样品进行成像的显微镜系统和方法。 在示例性方法中，可以针对第一组焦点位置检测透过样本的光，以收集第一叠图像。 可以为第一叠图像计算焦点度量的值。 候选焦点位置可以基于这些值来确定。 可从样品中检测第二组焦点位置的光致发光以收集第二叠图像。 第二组焦点位置可以定义比第一组焦点位置更小的范围。 第二组焦点位置的至少一个焦点位置可以基于候选焦点位置。 换句话说，候选焦点位置可以作为寻找合适的光致发光焦点位置的指导。
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公开(公告)号：WO2017045107A1
公开(公告)日：2017-03-23
申请号：PCT/CN2015/089549
申请日：2015-09-14
发明人： CAO, Peiyan , LIU, Yurun
CPC分类号： G01N21/6454 , B01J2219/00603 , B01J2219/00702 , C12Q1/6825 , G01N21/6428 , G01N21/6452 , G01N2021/6471
摘要： Disclosed herein is an apparatus (200, 500, 510, 520, 600, 700, 800, 900, 1000, 1100) comprising: a probe carrier (300, 310, 400, 410) comprising: a substrate (301, 401) comprising with holes (302, 402) through a thickness of the substrate (301, 401) and a transparent window (303) across an opening of each of the holes (302, 402), wherein the transparent window (303) closes the opening, wherein one or more locations on the transparent window (303) are configured to have probes (357, 457) attached thereto, wherein interaction between the probes (357, 457) and an analyte generates a signal; an optical system (285) comprising a plurality of collimators (295); a sensor (251) configured to detect the signal; wherein the collimators (295) can essentially prevent light from passing if a deviation of a propagation direction of the light from an optical axis of the collimators (295) is greater than a threshold. Because the probe carrier (300, 310, 400, 410) is separate and independent from the microarray (255), the probe carrier (300, 310, 400, 410) may be assembled with a pre-existing microarray (255) prior to its use, and be detached from the microarray (255) and disposed after its use.
摘要翻译： 本文公开了一种包括：探针载体（300,310,400,410）的装置（200,500,110,520,600,700,800,900,1000,1100），包括：基底（301,401），包括 具有通过所述基板（301,401）的厚度的孔（302,402）和穿过每个所述孔（302,402）的开口的透明窗（303），其中所述透明窗（303）封闭所述开口， 其中所述透明窗口（303）上的一个或多个位置被配置为具有附接到其上的探针（357,457），其中所述探针（357,457）和分析物之间的相互作用产生信号; 包括多个准直器（295）的光学系统（285）; 被配置为检测所述信号的传感器（251） 其中如果所述光从所述准直器（295）的光轴偏离的传播方向的偏差大于阈值，则所述准直器（295）可以基本上防止光通过。 因为探针载体（300,310,400,410）是与微阵列（255）分开且独立的，所以探针载体（300,310,400,410）可以在预先存在的微阵列（255）之前组装， 其使用，并与微阵列（255）分离，并在使用后放置。
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5.发明申请
公开(公告)号：WO2016178975A1
公开(公告)日：2016-11-10
申请号：PCT/US2016/030038
申请日：2016-04-29
申请人： SURE OPTICS, INC.
IPC分类号： G02B3/00
CPC分类号： G02B27/58 , B01L3/5085 , B01L2300/0654 , B01L2300/0822 , G01N21/0303 , G01N21/6452 , G01N2021/6439 , G01N2021/6478 , G01N2021/6482 , G02B3/0056 , G02B21/06 , G02B21/16 , G02B21/34
摘要： An optical imaging system with microlens array with integral structure includes a microlens array having a back surface for depositing sample material to be imaged and one or more microlenses on a front surface. At least one of the one or more microlenses are aligned to the deposited sample material. A plate is attached to the microlens array. A microscope objective is positioned proximate to the plurality of microlenses.
摘要翻译： 具有整体结构的具有微透镜阵列的光学成像系统包括微透镜阵列，该微透镜阵列具有用于沉积待成像的样品材料的背面和在前表面上的一个或多个微透镜。 一个或多个微透镜中的至少一个与沉积的样品材料对准。 平板连接到微透镜阵列。 显微镜物镜靠近多个微透镜定位。
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公开(公告)号：WO2016160131A1
公开(公告)日：2016-10-06
申请号：PCT/US2016/017181
申请日：2016-02-09
申请人： MULTERRA BIO, INC.
IPC分类号： C12M1/34
CPC分类号： G01N33/5438 , B01L3/502715 , B01L3/502761 , B01L2300/0645 , C12Q1/6825 , C12Q1/6834 , G01N21/553 , G01N21/6452 , G01N27/3276 , G01N33/48721
摘要： The present disclosure provides apparatuses and methods for analyzing the presence of charged analytes and/or the binding force between charged analytes and a capture probe. The apparatuses and methods of the present disclosure can be operated in a multiplexed format to perform various assays of clinical significance for example.
摘要翻译： 本公开提供了用于分析带电分析物的存在和/或带电分析物与捕获探针之间的结合力的装置和方法。 本公开的装置和方法可以以多路复用格式操作，以执行例如临床意义的各种测定。
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公开(公告)号：WO2016127124A3
公开(公告)日：2016-09-29
申请号：PCT/US2016016882
申请日：2016-02-05
发明人： CHU YONG , MARKS JEFFREY , FREUDENTHAL JACOB , WESSEL THOMAS , WOO DAVID
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q1/686 , G01N21/274 , G01N21/278 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G01N2021/6471 , G01N2201/127 , G01N2201/13 , G01N2333/922
摘要： In one exemplary embodiment, a method (100) for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration (102) to determine reaction site positions in an image. The method further includes performing a pure dye calibration (108) to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration (110) to determine a filter normalization factor. The method includes performing an RNase P validation (112) to validate the instrument is capable of distinguishing between two different quantities of sample.
摘要翻译： 在一个示例性实施例中，提供了一种用于校准仪器的方法（100）。 该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。 该方法包括执行感兴趣区域（ROI）校准（102）以确定图像中的反应位置位置。 该方法还包括通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来执行纯染料校准（108）以确定在每个反应位点中使用的荧光染料的贡献。 该方法还包括执行仪器归一化校准（110）以确定滤波器归一化因子。 该方法包括执行RNase P验证（112）以验证仪器能够区分两种不同数量的样品。
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公开(公告)号：WO2016127080A1
公开(公告)日：2016-08-11
申请号：PCT/US2016/016817
申请日：2016-02-05
发明人： CHU, Yong , FREUDENTHAL, Jacob , MARKS, Jeffrey
CPC分类号： C12Q1/686 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N2021/6421 , G01N2021/6441 , G01N2201/127
摘要： A computer-implemented method (500) for calibrating a fluorescent dye is described. The method (500) comprises imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder comprises a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method further comprises identifying a peak channel for each dye on the sample holder (508), normalizing each channel to the peak channel for each dye (510), and producing a dye matrix (518) that comprises a set of dye reference values.
摘要翻译： 描述了用于校准荧光染料的计算机实现的方法（500）。 方法（500）包括在多个通道上成像加载到仪器中的样品保持器。 样品架包括多个反应位点和多于一种染料类型，每种染料占据多于一个反应位点。 该方法还包括鉴定样品保持器（508）上的每种染料的峰值通道，将每个通道标准化为每个染料（510）的峰值通道，以及产生包含一组染料参考值的染料基质（518）。
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公开(公告)号：WO2016123157A1
公开(公告)日：2016-08-04
申请号：PCT/US2016/015002
申请日：2016-01-26
发明人： KIM, Jinho , YANG, Changhuei
CPC分类号： G02B21/365 , G01N21/6452 , G01N21/6456 , G01N21/6458 , G01N2021/6478 , G01N2201/0446 , G02B21/0076 , G02B21/16 , H04N5/2258
摘要： An imaging device for bright field Fourier ptychographic imaging and fluorescence imaging comprises a first fluorescence illumination source configured to provide excitation light of a first range of wavelengths to a transparent well; an optical system having a pair of objectives positioned oppositely; and an image sensor configured to receive light propagated through the optical system from the transparent well. The image sensor is configured to acquire a sequence of uniquely illuminated intensity measurements of light passing through a sample in the well based on sequential illumination at different illumination angles from a variable illumination source, and further configured to acquire a first fluorescence image of the sample based on light emitted by the sample in response to excitation light of the first range of wavelengths.
摘要翻译： 一种用于明场傅立叶印刷成像和荧光成像的成像设备，包括：第一荧光照射源，被配置为将第一波长范围的激发光提供给透明孔; 具有相对放置的一对物镜的光学系统; 以及图像传感器，被配置为接收从透明井传播通过光学系统传播的光。 图像传感器被配置为基于来自可变照明源的不同照射角度的顺序照射来获取穿过阱中样本的光的唯一照射强度测量的序列，并且还被配置为获取基于样本的第一荧光图像 对由样品响应于第一波长范围的激发光而发射的光进行测量。
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公开(公告)号：WO2016123156A1
公开(公告)日：2016-08-04
申请号：PCT/US2016/015001
申请日：2016-01-26
发明人： KIM, Jinho , YANG, Changhuei
CPC分类号： G02B21/365 , G01N21/6452 , G01N21/6456 , G01N21/6458 , G01N2021/6478 , G01N2201/0446 , G02B21/0076 , G02B21/16 , H04N5/2258
摘要： In one aspect an imaging system includes: an illumination system including an array of light sources; an optical system including one or more lens arrays, each of the lens arrays including an array of lenses, each of the lenses in each of the one or more lens arrays in alignment with a corresponding set of light sources of the array of light sources; an imaging system including an array of image sensors, each of the image sensors in alignment with a corresponding lens or set of lenses of the one or more lens arrays, each of the image sensors configured to acquire image data based on the light received from the corresponding lens or set of lenses; a plate receiver system capable of receiving a multi-well plate including an array of wells, the plate receiver system configured to align each of the wells with a corresponding one of the image sensors; and a controller configured to control the illumination of the light sources and the acquisition of image data by the image sensors, the controller further configured to perform: an image acquisition process including a plurality of scans, each scan associated with a unique pattern of illumination, each of the image sensors configured to generate an image for a respective one of the wells during each scan; and an image reconstruction process during which the controller performs a fourier ptychographic operation to generate a reconstructed image for each of the wells based on the image data captured for the respective well during each of the scans.
摘要翻译： 在一个方面，成像系统包括：包括光源阵列的照明系统; 包括一个或多个透镜阵列的光学系统，每个透镜阵列包括透镜阵列，所述一个或多个透镜阵列中的每一个中的每个透镜与所述光源阵列的相应组的光源对准; 一种成像系统，包括图像传感器阵列，每个图像传感器与一个或多个透镜阵列的相应透镜或透镜组对准，每个图像传感器被配置为基于从所述一个或多个透镜阵列接收的光获取图像数据 相应的镜头或镜片组; 板接收器系统，其能够接收包括孔阵列的多孔板，所述板接收器系统被配置为使每个所述孔与所述图像传感器中的相应一个对准; 以及控制器，其被配置为控制所述光源的照明和由所述图像传感器获取图像数据，所述控制器还被配置为执行：包括多个扫描的图像获取处理，每个扫描与唯一的照明模式相关联， 每个图像传感器被配置为在每次扫描期间为相应的一个孔产生图像; 以及图像重建过程，在该图像重建过程期间，控制器基于在每个扫描期间针对相应的阱捕获的图像数据执行傅立叶ptychographic操作以生成每个孔的重建图像。
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