公开(公告)号：WO2015066080A2

公开(公告)日：2015-05-07

申请号：PCT/US2014/062729

申请日：2014-10-28

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  SCHWARZ, Richard, I

Inventor： SCHWARZ, Richard, I

IPC: C07K14/775

CPC classification number: C07F9/5722 ,  B01D61/145 ,  B01D61/16 ,  C07K14/465 ,  C07K14/47 ,  C07K14/4705 ,  C07K16/18

Abstract: Herein is described a cell density signaling protein and its tissue-specific lipid cofactor. Constructs, compositions and methods for isolation, purification and use of the protein and the tissue-specific lipid cofactor are described.

Abstract translation: 本文描述了细胞密度信号蛋白及其组织特异性脂质辅因子。 描述了分离，纯化和使用蛋白质和组织特异性脂质辅因子的构建体，组合物和方法。

公开(公告)号：WO2015066080A3

公开(公告)日：2015-06-25

申请号：PCT/US2014062729

申请日：2014-10-28

Applicant: UNIV CALIFORNIA ,  SCHWARZ RICHARD I

Inventor： SCHWARZ RICHARD I

IPC: B01D15/00

CPC classification number: C07F9/5722 ,  B01D61/145 ,  B01D61/16 ,  C07K14/465 ,  C07K14/47 ,  C07K14/4705 ,  C07K16/18

Abstract: Herein we describe, a candidate gene for the diffusible cell density signal is put through the classic test to validate its activity: ectopic expression in another cell type from another species, purification of the recombinant factor, and testing back on PAT cells to see if it acts like the original diffusible cell density signal. This classic approach is complicated by the finding that the cell type producing this protein binds a unique tissue-specific lipid cofactor and this composite molecule imparts a tissue-specific response. Previously, this mechanism was shown to rely on a diffusible factor with an affinity for the cell layer.

Abstract translation: 在本文中，我们描述了扩散细胞密度信号的候选基因通过经典测试来验证其活性：来自另一物种的另一种细胞类型的异位表达，重组因子的纯化，以及在PAT细胞上测试 像原始扩散细胞密度信号一样。 这种经典方法通过发现产生该蛋白的细胞类型结合独特的组织特异性脂质辅因子的发现而变得复杂，并且该复合分子赋予组织特异性应答。 以前，这种机制显示依赖于对细胞层具有亲和性的扩散因子。