公开(公告)号：WO2010075061A2

公开(公告)日：2010-07-01

申请号：PCT/US2009068005

申请日：2009-12-15

Applicant: JOHNSON & JOHNSON RES PTY LTD ,  MACPHERSON JANET LESLEY ,  PERITT DAVID ,  HENRICHSEN KEVIN ,  SYMONDS GEOFFREY PHILLIP ,  POND SUSAN ,  WONG PHILIP

Inventor： MACPHERSON JANET LESLEY ,  PERITT DAVID ,  HENRICHSEN KEVIN ,  SYMONDS GEOFFREY PHILLIP ,  POND SUSAN ,  WONG PHILIP

IPC: A61M1/34

CPC classification number: A61M1/3496 ,  A61M1/362 ,  A61M1/3626 ,  A61M1/3672 ,  A61M1/3689 ,  A61M1/3696 ,  A61M1/38 ,  A61M2202/0439 ,  B03C1/015 ,  B04B5/10 ,  B04B11/02 ,  A61M2202/0064

Abstract: Methods (300), devices, and systems of processing blood are described. The method (300) comprises the steps of: obtaining (312) blood from a patient coupled to a single blood processing device to form a closed loop between the patient and the blood processing device; collecting (314) bulk mononuclear blood cells from the blood by leukapheresis implemented using the blood processing device in the closed loop; and enriching (316) concurrently target cells separated from non-target cells in the bulk mononuclear blood cells using the blood processing device in the closed loop.

Abstract translation: 描述了方法（300），装置和血液处理系统。 该方法（300）包括以下步骤：从耦合到单个血液处理装置的患者获得（312）血液以在患者和血液处理装置之间形成闭环; 通过使用血液处理装置在闭环中实施的白细胞分离术从血液收集（314）大量单核血细胞; 以及使用闭环中的血液处理装置同时富集（316）在大批量单核血细胞中从非靶细胞分离的靶细胞。

公开(公告)号：WO1997042336A1

公开(公告)日：1997-11-13

申请号：PCT/AU1997000280

申请日：1997-05-06

Applicant: UNISEARCH LIMITED ,  MacKENZIE, Karen, Lee ,  SYMONDS, Geoffrey, Phillip

Inventor： UNISEARCH LIMITED

IPC: C12N15/86

CPC classification number: C12N15/86 ,  A61K48/00 ,  C12N2740/13043

Abstract: The present invention provides a recombinant retroviral vector which is replication-defective and includes a long terminal repeat (LTR) sequence for expression of a heterologous polynucleotide sequence, wherein the vector does not include a heterologous promoter element or a heterologous gene which imparts antibiotic or other resistance. The retroviral vector may be used to introduce foreign genes into mammalian cells and can be used for therapeutic purposes.

Abstract translation: 本发明提供了一种重组逆转录病毒载体，其是复制缺陷型的并且包括用于表达异源多核苷酸序列的长末端重复序列（LTR）序列，其中所述载体不包括赋予抗生素或其它的异源启动子元件或异源基因 抵抗性。 逆转录病毒载体可用于将外源基因引入哺乳动物细胞中，并可用于治疗目的。

公开(公告)号：WO2010075061A3

公开(公告)日：2010-07-01

申请号：PCT/US2009/068005

申请日：2009-12-15

Applicant: JOHNSON & JOHNSON RESEARCH PTY LIMITED ,  MACPHERSON, Janet, Lesley ,  PERITT, David ,  HENRICHSEN, Kevin ,  SYMONDS, Geoffrey, Phillip ,  POND, Susan ,  WONG, Philip

Inventor： MACPHERSON, Janet, Lesley ,  PERITT, David ,  HENRICHSEN, Kevin ,  SYMONDS, Geoffrey, Phillip ,  POND, Susan ,  WONG, Philip

IPC: A61M1/34

Abstract: Methods (300), devices, and systems of processing blood are described. The method (300) comprises the steps of: obtaining (312) blood from a patient coupled to a single blood processing device to form a closed loop between the patient and the blood processing device; collecting (314) bulk mononuclear blood cells from the blood by leukapheresis implemented using the blood processing device in the closed loop; and enriching (316) concurrently target cells separated from non-target cells in the bulk mononuclear blood cells using the blood processing device in the closed loop.