公开(公告)号：WO1986005202A1

公开(公告)日：1986-09-12

申请号：PCT/US1986000260

申请日：1986-02-06

Applicant: VERAX CORPORATION

Inventor： VERAX CORPORATION ,  DEAN, Robert, C., Jr. ,  GRELA, Peter, V. ,  KARKARE, Subhash, B.

IPC: C12N05/00

CPC classification number: C12P7/06 ,  C07K14/59 ,  C12M23/58 ,  C12M25/20 ,  C12M27/02 ,  C12N2531/00 ,  Y02E50/17

Abstract: A fluidized bed reactor (100) and reaction process, particularly well-suited for culturing cells, for example, for tissue culture and fermentation processes, which involve the treatment of at least a portion of the fluid exiting the fluidized bed reactor (100) in a side loop (160) in a manner to alter its temperature or composition, e.g., oxygenation, with recirculation of this treated fluid to the reactor as a portion of the fluid causing bed fluidization.

Abstract translation: 流化床反应器（100）和反应方法，特别适用于培养细胞，例如用于组织培养和发酵过程，其涉及处理离开流化床反应器（100）的至少一部分流体 一种侧环（160）以改变其温度或组成（例如氧合）的方式，将该处理过的流体再循环到反应器中作为导致床流化的流体的一部分。

公开(公告)号：WO1986005811A1

公开(公告)日：1986-10-09

申请号：PCT/US1986000600

申请日：1986-03-26

Applicant: VERAX CORPORATION

Inventor： VERAX CORPORATION ,  DEAN, Robert, C. ,  PHILLIPS, Philip, G. ,  RUNSTADLER, Peter, W., Jr. ,  SILVER, Frederick, H. ,  BERG, Richard, A. ,  CAHN, Frederick

IPC: C12P21/00

CPC classification number: C12N1/00 ,  C12N5/0075 ,  C12N11/02 ,  C12N2533/54 ,  C12P1/00

Abstract: Weighted microsponges preferably having a highly crosslinked collagen matrix suitable for use in culturing organisms in motive reactor systems. The microsponges have an open to the surface pore structure, and pore sizes and volumes suitable for immobilizing a variety of bioactive materials. The microsponges also have an average particle size in the range of about 100 to about 1000 microns and a specific gravity above about 1.05. Also described is an in vitro method for culturing organisms immobilized on a microsponge comprising a highly crosslinked, porous collagen matrix prepared by crosslinking Type I, II or III type collagen using a carbodiimide or a succinimidyl active ester and/or severe dehydration conditions.

Abstract translation: 加重的微球优选具有适用于在动力反应器系统中培养生物体的高度交联的胶原基质。 微球具有开放的表面孔结构，并且适合于固定各种生物活性材料的孔径和体积。 微球也具有约100至约1000微米范围内的平均粒度和约1.05的比重。 还描述了用于培养固定在微球上的生物体的体外方法，其包括通过使用碳二亚胺或琥珀酰亚胺基活性酯交联I型，II型或III型胶原和/或严重脱水条件制备的高度交联的多孔胶原基质。