公开(公告)号：WO2012139586A1

公开(公告)日：2012-10-18

申请号：PCT/DK2012/050120

申请日：2012-04-11

Applicant: PROXEON BIOSYSTEMS A/S ,  VORM, Ole

Inventor： VORM, Ole

IPC: G01N30/60 ,  B01D15/22

CPC classification number: B01D15/22 ,  B29D23/00 ,  G01N30/60 ,  G01N30/6004 ,  G01N30/6039 ,  G01N2030/3061 ,  G01N2030/6013

Abstract: There is provided a capillary assembly suitable for connecting various components of an analytical measuring device, for example a liquid chromatograph or a capillary electrophoresis device, to each other. Specifically, this involves reinforcing the fragile tubing by the addition of PEEK or steel sleeves and/or embedding the tubing in an injection-molded resin such that the tubing is not exposed directly to operator handling and manipulation. Further functional improvement is obtained by including additional components inside the resin. Thereby a versatile and robust capillary assembly is achieved.

Abstract translation: 提供了一种适于将分析测量装置（例如液相色谱仪或毛细管电泳装置）的各种组件彼此连接的毛细管组件。 具体地说，这涉及通过添加PEEK或钢套筒来加强脆性管道，和/或将管道嵌入到注塑树脂中，使得管道不直接暴露于操作员处理和操作。 通过在树脂内部包含额外的组分来获得进一步的功能改进。 从而实现了多功能和坚固的毛细管组件。

公开(公告)号：WO2009147001A1

公开(公告)日：2009-12-10

申请号：PCT/EP2009/055733

申请日：2009-05-12

Applicant: PROXEON BIOSYSTEMS A/S ,  VORM, Ole ,  THEODORSEN, Soeren ,  AAGAARD NIELSEN, Peter

Inventor： VORM, Ole ,  THEODORSEN, Soeren ,  AAGAARD NIELSEN, Peter

IPC: B29C45/14 ,  B01D15/22 ,  G01N30/60

CPC classification number: G01N30/7266 ,  B01D15/22 ,  B29C45/14377 ,  B29C45/14426 ,  G01N30/6004 ,  G01N30/6047 ,  G01N30/6052 ,  G01N30/6091

Abstract: There is provided an integrated system for liquid separation, such as LC, CE, affinity chromatography, and ion exchange chromatography, comprising a column and end-fittings embedded in a plastic material, such as a thermoplastic polymer. The system may further comprise an electrospray emitter directly connected with the outlet of the column, wherein a substantial part of the emitter is covered with the polymer material. There is also provided a method by which a separation column along with the accompanying end fittings for connection with adjacent liquid conduits is embedded in a polymer matrix. This configuration e.g. ensures that the factory-made, correct attachment of the fittings to the column is preserved (since the matrix prevents further user intervention, accidental or otherwise).

Abstract translation: 提供了用于液体分离的集成系统，例如LC，CE，亲和层析和离子交换层析，其包括嵌入塑料材料（例如热塑性聚合物）中的柱和端接头。 该系统还可以包括与柱的出口直接连接的电喷射发射器，其中大部分发射器被聚合物材料覆盖。 还提供了一种方法，通过该方法将分离柱以及用于与相邻液体管道连接的伴随端部配件嵌入聚合物基质中。 这种配置例如 确保将配件的工厂制造的正确附件保留在柱子上（因为矩阵防止进一步的用户干预，意外或其他）。

公开(公告)号：WO2009010379A3

公开(公告)日：2009-10-22

申请号：PCT/EP2008058232

申请日：2008-06-27

Applicant: PROXEON BIOSYSTEMS AS ,  VORM OLE ,  GRAF THOMAS

Inventor： VORM OLE ,  GRAF THOMAS

IPC: H01J49/16

CPC classification number: H01J49/16 ,  H01J49/167

Abstract: The present invention provides a novel nanospray emitter that includes a fluid inlet, an outlet orifice, and a passage for fluid: communication between the fluid inlet and outlet orifice. In one aspect of this embodiment, the passage facilitating fluid communication between the inlet and outlet elements is comprised of an outer capillary of a rigid material, such as nickel, that houses an inner capillary made from a highly conductive and inert material, such as gold; alternatively the inner material may constitute an inner coating of the outer capillary.

Abstract translation: 本发明提供了一种新颖的纳米喷雾发射器，其包括流体入口，出口孔和用于流体的通道：流体入口和出口孔之间的连通。 在该实施例的一个方面，促进入口和出口元件之间的流体连通的通道包括刚性材料（例如镍）的外部毛细管，其容纳由高导电性和惰性材料制成的内部毛细管，例如金 ; 或者，内部材料可以构成外部毛细管的内部涂层。

公开(公告)号：WO2009098125A8

公开(公告)日：2009-10-01

申请号：PCT/EP2009050697

申请日：2009-01-22

Applicant: PROXEON BIOSYSTEMS AS ,  VORM OLE

Inventor： VORM OLE

IPC: B01D15/16 ,  G01N30/32 ,  G01N30/36

CPC classification number: G01N30/32 ,  B01D15/163 ,  G01N30/36 ,  G01N2030/324 ,  G01N2030/326

Abstract: A method for controlling the flow of liquid in a high performance liquid chromatography apparatus. The method includes operating a pump, measuring the liquid pressure downstream of the pump, measuring the liquid flow rate downstream of the pump, and controlling the operation of the pump. In the method, it is automatically determined whether the pump is controlled to achieve a desired pressure or controlled to achieve a desired flow rate. Fuzzy logic can be applied in the method to determine the switch between the control modes.

Abstract translation: 一种控制高效液相色谱仪中液体流动的方法。 该方法包括操作泵，测量泵下游的液体压力，测量泵下游的液体流速，并控制泵的运行。 在该方法中，自动确定泵是否被控制以实现期望的压力或被控制以实现期望的流量。 模糊逻辑可以用于确定控制模式之间切换的方法。

公开(公告)号：WO2009010379A2

公开(公告)日：2009-01-22

申请号：PCT/EP2008/058232

申请日：2008-06-27

Applicant: Proxeon Biosystems A/S ,  VORM, Ole ,  GRAF, Thomas

Inventor： VORM, Ole ,  GRAF, Thomas

IPC: H01J49/16

CPC classification number: H01J49/16 ,  H01J49/167

Abstract: The present invention provides a novel nanospray emitter that includes a fluid inlet, an outlet orifice, and a passage for fluid: communication between the fluid inlet and outlet orifice. In one aspect of this embodiment, the passage facilitating fluid communication between the inlet and outlet elements is comprised of an outer capillary of a rigid material, such as nickel, that houses an inner capillary made from a highly conductive and inert material, such as gold; alternatively the inner material may constitute an inner coating of the outer capillary.

Abstract translation: 本发明提供了一种新颖的纳流喷雾发射器，其包括流体入口，出口孔和用于流体的通道：流体入口和出口孔之间的连通。 在该实施例的一个方面，促进入口和出口元件之间的流体连通的通道包括诸如镍的刚性材料的外部毛细管，其容纳由高导电性和惰性材料例如金制成的内部毛细管 ; 或者内部材料可以构成外部毛细管的内部涂层。

公开(公告)号：WO0190194A3

公开(公告)日：2002-06-20

申请号：PCT/EP0105943

申请日：2001-05-23

Applicant: ROCHE DIAGNOSTICS GMBH ,  HOFFMANN LA ROCHE ,  HOESEL WOLFGANG ,  PETER JOCHEN ,  VORM OLE ,  KROGH THOMAS N

Inventor： HOESEL WOLFGANG ,  PETER JOCHEN ,  VORM OLE ,  KROGH THOMAS N

IPC: G01N33/577 ,  C07K5/10 ,  C07K7/06 ,  C07K16/18 ,  C07K16/40 ,  C12N15/02 ,  C12P21/08 ,  G01N33/574 ,  C07K16/30 ,  C07K14/705

CPC classification number: G01N33/57434 ,  C07K16/40 ,  G01N2333/8132

Abstract: The invention relates to antibodies against particular forms of enzymatically-inactive precursors of the prostate specific antigen (PSA), the production and use thereof in immunoassays for the determination of the [-5,-6,-7]-proPSA and application of the measured values from said assays, for an improved diagnosis in the field of prostate carcinomas.

Abstract translation: 本发明涉及针对前列腺特异性抗原的无酶活性的前体的特定形式（PSA），它们的制备，它们在免疫测定使用用于测定抗体[-5，-6，-7] proPSA，并从这些测定中使用的测定值的 在前列腺癌领域改进的诊断。

公开(公告)号：WO2010057826A1

公开(公告)日：2010-05-27

申请号：PCT/EP2009/065041

申请日：2009-11-12

Applicant: PROXEON BIOSYSTEMS A/S ,  VORM, Ole

Inventor： VORM, Ole

IPC: G01N30/08 ,  G01N30/30

CPC classification number: G01N30/08 ,  G01N30/30 ,  G01N2030/085 ,  G01N2030/122 ,  G01N2030/3015 ,  G01N2030/303 ,  G01N2030/3076

Abstract: A method is provided for thermally focusing an analyte on a pre-column for liquid chromatography, comprising the steps of enriching the sample on said pre-column at a temperature lower than the operating temperature of a separation column, injecting the sample onto the separation column by heating the pre-column to a temperature higher than the temperature of the separation column and eluting the analyte from the separation column. Optionally, the sample may be subjected to a temperature gradient on the separation column or the separation column may be heated to remove non-eluted sample after the separation is completed.

Abstract translation: 提供了一种用于将分析物热聚焦在用于液相色谱的预柱上的方法，包括以下步骤：在低于分离塔的操作温度的温度下将样品在所述预柱上富集，将样品注入到分离柱 通过将预柱加热到高于分离塔的温度的温度，并从分离塔中洗脱分析物。 任选地，样品可以在分离塔上经受温度梯度，或者可以在分离完成之后加热分离柱以除去未洗脱的样品。

公开(公告)号：WO2009098125A1

公开(公告)日：2009-08-13

申请号：PCT/EP2009/050697

申请日：2009-01-22

Applicant: PROSEON BIOSYSTEMS A/S ,  VORM, Ole

Inventor： VORM, Ole

IPC: B01D15/16 ,  G01N30/32 ,  G01N30/36

CPC classification number: G01N30/32 ,  B01D15/163 ,  G01N30/36 ,  G01N2030/324 ,  G01N2030/326

Abstract: A method for controlling the flow of liquid in a high performance liquid chromatography apparatus. The method includes operating a pump, measuring the liquid pressure downstream of the pump, measuring the liquid flow rate downstream of the pump, and controlling the operation of the pump. In the method, it is automatically determined whether the pump is controlled to achieve a desired pressure or controlled to achieve a desired flow rate. Fuzzy logic can be applied in the method to determine the switch between the control modes.

Abstract translation: 用于控制高效液相色谱装置中的液体流动的方法。 该方法包括操作泵，测量泵下游的液体压力，测量泵下游的液体流量，以及控制泵的操作。 在该方法中，自动确定泵是否被控制为实现期望的压力或被控制以实现期望的流量。 模糊逻辑可应用于确定控制模式之间切换的方法。