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公开(公告)号：WO2006040358A3
公开(公告)日：2007-03-01
申请号：PCT/EP2005055316
申请日：2005-10-17
申请人： DSM IP ASSETS BV , SAGT CORNELIS MARIA JACOBUS , WINDE DE JOHANNES HENDRIK , WENZEL THIBAUT JOSE , VONK BRENDA
IPC分类号： C12N9/80
CPC分类号： C12N9/80
摘要： The present invention relates to novel functional amdS genes from A.niger that can be used as dominant and bi-directional selection marker gene in the transformation of organisms. The present invention further relates to the production of a compound of interest in a fungal host cell transformed with the amdS genes of the invention. Preferred fungal host cells are filamentous fungal cells. The amdS genes of the invention provide means for identification of functional homologues in other Aspergillus species.
摘要翻译： 本发明涉及可以用作生物体转化中的显性和双向选择标记基因的来自黑曲霉的新型功能性amdS基因。 本发明还涉及在用本发明的amdS基因转化的真菌宿主细胞中生产感兴趣的化合物。 优选的真菌宿主细胞是丝状真菌细胞。 本发明的amdS基因提供了用于鉴定其他曲霉属中功能同系物的方法。
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公开(公告)号：WO2006097539A3
公开(公告)日：2006-11-30
申请号：PCT/EP2006060864
申请日：2006-03-20
申请人： DSM IP ASSETS BV , MEIMA ROELF BERNHARD , WENZEL THIBAUT JOSE , SAGT CORNELIS MARIA JACOBUS , DE WINDE JOHANNES HENDRIK , KUIPERS OSCAR PAUL , ROOIJEN VAN RUTGER JAN , DEKKER MARJOLEIN CONELIA , VONK BRENDA
发明人： MEIMA ROELF BERNHARD , WENZEL THIBAUT JOSE , SAGT CORNELIS MARIA JACOBUS , DE WINDE JOHANNES HENDRIK , KUIPERS OSCAR PAUL , ROOIJEN VAN RUTGER JAN , DEKKER MARJOLEIN CONELIA , VONK BRENDA
IPC分类号： C12N15/09
CPC分类号： C12N15/1086 , C07K14/32 , C07K14/38 , C12N15/80
摘要： DSM IP Assets B.V. 24403WO IMPROVED METHOD OF EXPRESSION CLONING IN A HOST CELL 5 ABSTRACT The invention relates to a promoter DNA sequence highly suited in an improved expression cloning method for isolation of DNA sequences comprising a DNA sequence encoding a protein of interest in a host cell and to the improved expression cloning method wherein use is made of this promoter. The isolated DNA sequences are useful in processes for 10 producing a protein of interest.
摘要翻译： 在宿主细胞中5摘要本发明涉及非常适合于改进的表达克隆方法，其包括在宿主细胞中编码目的蛋白的DNA序列的DNA序列的分离的启动子DNA序列的表达克隆DSM IP资产BV 24403WO改进方法 并涉及其中使用该启动子的改进的表达克隆方法。 分离的DNA序列可用于产生感兴趣的蛋白质的过程。
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3.发明申请USE OF OXALATE DEFICIENT ASPERGILLUS NIGER STRAINS FOR PRODUCING A POLYPEPTIDE 审中-公开草酸缺陷型黑曲霉菌产生多肽的应用
公开(公告)号：WO2004070022A3
公开(公告)日：2004-10-28
申请号：PCT/EP2004001173
申请日：2004-02-05
摘要： The invention relates to oxalate deficient A. niger strains for the production of a given enzyme, wherein the oxalate deficient strain produces at least the same amount of enzyme as the wild type strain it originates from under the same culture conditions. Preferably, the oxalate deficient A. niger strain produces more enzyme than the wild type strain it originates from under the same culture conditions. More preferably, the oxalate deficient A. niger strain is such that when the strain has been transformed with an expression construct comprising a gene coding for an enzyme, said strain produces at least the amount of the enzyme the wild type strain it originates from would produce under the same culture conditions, when the wild type strain has also been transformed with the same expression construct as the oxalate deficient strain. The invention also relates to method for obtaining such oxalate deficient A. niger strain. The present invention further relates to method for producing an enzyme, wherein an oxalate deficient A. niger strain that produces at least the same amount of enzyme as the wild type strain it originates from under the same culture conditions is used.
摘要翻译： 本发明涉及用于生产给定酶的草酸盐缺陷型黑曲霉菌株，其中所述草酸盐缺陷型菌株在相同培养条件下产生与源自其的野生型菌株至少相同量的酶。 优选地，草酸缺陷的黑曲霉菌株产生比它在相同培养条件下来源的野生型菌株更多的酶。 更优选地，草酸缺陷的黑曲霉菌株是这样的，即当该菌株已经用包含编码酶的基因的表达构建体转化时，所述菌株产生至少一定量的源自其的野生型菌株的酶将产生 在相同的培养条件下，当野生型菌株也已经用与草酸盐缺陷菌株相同的表达构建体转化时。 本发明还涉及获得这种草酸盐缺陷的黑曲霉菌株的方法。 本发明进一步涉及生产酶的方法，其中使用在相同培养条件下产生与源自其的野生型菌株至少相同量的酶的草酸盐缺陷型黑曲霉菌株。
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公开(公告)号：WO2008017483A3
公开(公告)日：2008-04-10
申请号：PCT/EP2007007052
申请日：2007-08-09
CPC分类号： C12N15/75 , A23K10/16 , A23K10/18 , A23K20/189 , A61K38/00 , C07K14/32 , C07K14/33 , C07K14/38 , C12N9/16 , C12Y301/03001 , C12Y301/03008 , C12Y301/03026 , C12Y302/01031
摘要： This invention discloses novel bacterial spore systems. It has now been found surprisingly that under certain conditions bacterial spore systems can be used in the food and feed industry, preferably in animal feeding and as biohybrid material. More precisely applicant has found the following: Genetically modified or genetically engineered viable spore systems expressing bioactive polypeptides, for example bacteriocins and/or enzymatically active feed enzymes, at the spore surface, have a great potential use in animal feeding. Further, it has been found that genetically modified or "genetically engineered inert spore systems expressing affinity ligands or immobilized enzymes at the surface have a great potential use in biocatalysis and in the construction of biocatalytic films. Especially the resistance to harsh chemicals, desiccation, strong pressure, or high temperatures allows the spores to be a potentially valuable tool for the display of bioactive molecules, like biocatalytic enzymes or bioactive feed enzymes that must survive harsh conditions to deliver their full potential. Finally, applicant has found that instead of translational fusions to spore structural genes as it is known from the prior art described above, passenger bioactive polypeptides, as for example enzymes, bacteriocins, affinity ligands, can also be fused to spore-specific surface enzymes, for example to spore specific enzymes as mentioned herein above.
摘要翻译： 本发明公开了新的细菌孢子系统。 现在令人惊讶地发现，在某些条件下，细菌孢子系统可以用于食品和饲料工业，优选用于动物饲养和作为生物混杂材料。 更确切地说，申请人已经发现：在孢子表面表达生物活性多肽，例如细菌素和/或酶活性饲料酶的基因修饰或基因工程活的孢子系统在动物饲养中具有很大的潜在用途。 此外，已经发现基因修饰或“基因工程惰性孢子系统在表面表达亲和配体或固定化酶在生物催化和生物催化膜的构建中具有很大的潜在用途，特别是耐受苛刻的化学品，干燥，强 压力或高温使得孢子成为展示生物活性分子的潜在有价值的工具，如生物催化酶或生物活性饲料酶，其必须在苛刻的条件下存活以充分发挥其潜力。最后，申请人发现，代替平移融合 孢子结构基因，如从上述现有技术中已知的那样，乘客生物活性多肽（例如酶，细菌素，亲和配体）也可以与孢子特异性表面酶融合，例如芽孢如上所述的特异性酶。
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公开(公告)号：WO2005100573A9
公开(公告)日：2007-11-22
申请号：PCT/EP2005051696
申请日：2005-04-18
CPC分类号： C07K14/38
摘要： The present invention relates to isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding polypeptides. The present invention also relates to methods for expressing a gene and/or producing a polypeptide using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.
摘要翻译： 本发明涉及分离的真菌启动子DNA序列，DNA构建体，载体和包含这些启动子的真菌宿主细胞，其与编码多肽的编码序列有效结合。 本发明还涉及使用分离的新启动子来表达基因和/或产生多肽的方法。 本发明还涉及使用本发明的新启动子改变内源基因的转录水平和/或调节的方法。
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公开(公告)号：WO2005100573A2
公开(公告)日：2005-10-27
申请号：PCT/EP2005051696
申请日：2005-04-18
CPC分类号： C07K14/38
摘要： The present invention relates to isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding polypeptides. The present invention also relates to methods for expressing a gene and/or producing a polypeptide using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.
摘要翻译： 本发明涉及分离的真菌启动子DNA序列，DNA构建体，载体和包含这些启动子的真菌宿主细胞，其与编码多肽的编码序列有效结合。 本发明还涉及使用分离的新启动子来表达基因和/或产生多肽的方法。 本发明还涉及使用本发明的新启动子改变内源基因的转录水平和/或调节的方法。
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公开(公告)号：WO2005067735A2
公开(公告)日：2005-07-28
申请号：PCT/EP2005000442
申请日：2005-01-13
申请人： DSM IP ASSETS BV , MUTSAERS JOHANNA HENRICA GERDI , WENZEL THIBAUT JOSE , DE BOER LEX , DIJK VAN ALBERTUS ALARD , ROOIJEN VAN RUTGER JAN
发明人： MUTSAERS JOHANNA HENRICA GERDI , WENZEL THIBAUT JOSE , DE BOER LEX , DIJK VAN ALBERTUS ALARD , ROOIJEN VAN RUTGER JAN
IPC分类号： A23L5/40 , A21D8/04 , A23C19/032 , A23L5/49 , A23L1/27
CPC分类号： A23L1/27 , A21D8/042 , A23C19/0328 , A23L5/40 , A23V2002/00 , C11D3/386 , C11D3/40 , A23V2200/046
摘要： The present invention relates to a process for the production of a food product whereby an intermediate form of said food product comprises a pigment, which process comprises adding at least one enzyme that is effective in directly converting said pigment into a form which results in increasing the whiteness of at least part of the food product compared to the food product for which said enzyme is not added during its production. The invention also relates to food products obtained from the process of the invention.
摘要翻译： 本发明涉及生产食品的方法，其中所述食品的中间形式包含颜料，该方法包括加入至少一种有效直接将所述颜料转化成导致增加 与在其生产期间不添加所述酶的食品相比，至少部分食品的白度。 本发明还涉及从本发明的方法获得的食品。
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