摘要:
Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described.
摘要:
The description relates to, inter alia, recombinant microorganisms, engineered metabolic pathways, chemical catalysts, and products produced through the use of the described methods and materials. The products produced include functionalized alpha substituted C4 dicarboxylic acids, as well as functionalized malonyl-CoA, malonic semialdehyde, and acrylic acids, and their salts, esters and lactones.
摘要:
Transgenic plant species engineered to inhibit aflatoxin production in Aspergillus species, methods of producing such transgenic plant species that can inhibit aflatoxin production, and compositions for such transgenic plant production. The transgenic plants harbor an RNAi cassette expressing dsRNA for an enzyme, such as aflC, that is required for aflatoxin biosynthesis. The dsRNA may include at least one, at least two, at least three, or more than three sections of the target enzyme gene.
摘要:
The present invention provides hybrid polypeptides having cellobiohydrolase activity. The present invention also provides polynucleotides encoding the hybrid polypeptides; nucleic acid constructs, vectors and host cells comprising the polynucleotides; and processes of using the hybrid polypeptides.
摘要:
The present invention provides an isolated fungal cell that is capable of producing one or more biomass-degrading enzymes and that exhibits increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. Also provided is a fermentation processes for producing one or more biomass-degrading enzymes comprising a fungal cells exhibiting increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. The biomass-degrading enzymes produced by the isolate fungal cell and fermentation processes of the present invention may be used in a process to produce soluble sugars from biomass.
摘要:
The present invention provides a microorganism capable of fermenting arabinose to a desired product such as ethanol. In some embodiments, the organism is also capable of fermenting xylose. In some embodiments, the organism is capable of fermenting arabinose and xylose, and expresses one or more cellulases.
摘要:
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要:
The invention relates to the production of biofuels, proteins, peptides and other value-added compounds from crude carbon sources. The inventors identified genes encoding novel pentose transporters, in particular transporters of L-arabinose and/or D-xylose. Regulation of the Aspergillus niger genes by xlnR and araR was instrumental in the identification of these genes and their substrate specificities. Provided are novel pentose transporters and their encoding nucleic acids. Also provided are host cells (over)expressing a transporter, and industrial applications thereof, for instance in biofuel production.
摘要翻译:公开的是:能够调节甘露聚糖酶或纤维素酶基因的转录或表达的转录调节因子,如下所述; 和别的。 具体公开的是选自以下蛋白质(a),(b)和(c)的蛋白质:(a)包含SEQ ID NO:2所示氨基酸序列的蛋白质; (b)包含在SEQ ID NO:2所示的氨基酸序列中缺失,取代或添加一个或几个氨基酸残基(例如1至5个氨基酸残基)而产生的氨基酸序列的蛋白质,其能够 调节甘露聚糖酶或纤维素酶基因的转录; 和(c)蛋白质,其包含与SEQ ID NO:2所示的氨基酸序列具有70%或更高序列同一性的氨基酸序列,并且能够调节甘露聚糖酶或纤维素酶的基因的转录,或部分 蛋白质的片段。 还具体公开了编码蛋白质的基因等。