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    INDUCTION OF XA27 BY THE AVRXA27 GENE IN RICE CONFERS BROAD-SPECTRUM RESISTANCE TO XANTHOMONAS ORYZAE PV. ORYZAE AND ENHANCED RESISTANCE TO XANTHOMONAS ORYZAE PV. ORYZICOLA
    1.
    发明申请
    INDUCTION OF XA27 BY THE AVRXA27 GENE IN RICE CONFERS BROAD-SPECTRUM RESISTANCE TO XANTHOMONAS ORYZAE PV. ORYZAE AND ENHANCED RESISTANCE TO XANTHOMONAS ORYZAE PV. ORYZICOLA 审中-公开
    通过AVRXA27基因对XANTHOMONAS ORYZAE PV的广谱抗性的影响，诱导XA27。 ORYZAE和增强电阻对XANTHOMONAS ORYZAE PV。 空心稗

    公开(公告)号：WO2008094127A1

    公开(公告)日：2008-08-07

    申请号：PCT/SG2008/000005

    申请日：2008-01-08

    申请人： TEMASEK LIFE SCIENCES LABORATORY LIMITED ,  YIN, Zhongchao ,  TIAN, Dongsheng

    发明人： YIN, Zhongchao ,  TIAN, Dongsheng

    IPC分类号： A01H5/00

    CPC分类号： C12N15/8281

    摘要： The present invention generally provides a method to generate broad-spectrum resistance to bacterial blight disease in plants. More specifically, the present invention provides a method to generate broad-spectrum resistance to Xanthomonas oryzae pv. oryzae , the causal agent of bacterial blight disease of rice, and enhanced resistance to Xanthomonas oryzae pv. oryzicola , the causal agent of bacterial leaf streak of rice. Xa27 , an inducible bacterial blight R gene in rice, was induced by the cognate avrXa27 gene expressed in host. Rice plants carrying the avrXa27 transgene and wild-type Xa27 gene conferred resistance to incompatible and compatible pathogens, and enhanced resistance to X. oryzae pv. oryzicola strain L8. The Xa27 -mediated enhanced resistance to X. oryzae pv. oryzicola was also observed in the interaction between IRBB27 and L8 harboring pHM1avrXa27. This was further verified by the fact that the Xa27 gene in IRBB27 was induced by the avrXa27 gene in bacteria. The method can be used to engineer broad-spectrum resistance of rice to bacterial blight and enhanced resistance to bacterial leaf streak. Slight modification of this technique can be applied to control bacterial diseases in other crops.

    摘要翻译： 本发明通常提供了一种在植物中产生广谱抗病菌性疾病的方法。 更具体地说，本发明提供了产生对黄单胞菌属（Xanthomonas oryzae）pv的广谱抗性的方法。 稻谷细菌性枯萎病的致病因子和米黄单胞菌的抗性增强。 稻瘟病菌是水稻细菌条纹病原体。 Xa27是水稻中诱导性的细菌性枯萎病R基因，由宿主中表达的同源avrXa27基因诱导。 携带avrXa27转基因和野生型Xa27基因的水稻植物赋予对不相容和相容的病原体的抗性，并增强了对米曲霉的抵抗力。 米曲霉菌株L8。 Xa27介导的对米曲霉的增强的抗性 在含有pHM1avrXa27的IRBB27和L8之间的相互作用中也观察到稻瘟病菌。 通过细菌中的avrXa27基因诱导IRBB27中的Xa27基因的事实进一步证实了这一点。 该方法可用于将稻米的广谱抗性设计成细菌性枯萎病，提高抗细菌性条纹的抗性。 该技术的轻微修改可用于控制其他作物的细菌性疾病。