公开(公告)号：WO2012007445A1

公开(公告)日：2012-01-19

申请号：PCT/EP2011/061813

申请日：2011-07-12

Applicant: DSM IP Assets B.V. ,  DEKKER, Petrus, Jacobus, Theodorus ,  ZEEMAN, Marjan Petra

Inventor： DEKKER, Petrus, Jacobus, Theodorus ,  ZEEMAN, Marjan Petra

IPC: C12P19/12 ,  C12P41/00 ,  C13K5/00 ,  C12P19/02 ,  C12N9/90

CPC classification number: C12P19/12 ,  C12N9/90 ,  C12P41/00 ,  C12Y501/03003 ,  C13K5/00 ,  C13K13/002

Abstract: This invention describes the use of mutarotase (also known as aldose-1-epimerase) in a process for carbohydrate crystallization. Preferably the carbohydrate is a reducing sugar, more preferably the carbohydrate is lactose. The mutarotase can be obtained from an organism, preferably a micro-organism, more preferably from a fungus, even more preferably from a fungus of the genus Aspergillus, most preferably from Aspergillus niger. Carbohydrate crystallization can be performed from a pure, partly purified or a crude solution of carbohydrate. In general, carbohydrates are first concentrated into a supersaturated solution, after which temperature decreases and optional seeding with carbohydrate crystals initiate crystallization. Mutarotase is preferably added before or during the crystallization process.

Abstract translation: 本发明描述了在碳水化合物结晶的过程中使用灭活酶（也称为醛糖-1-差向异构酶）。 优选地，碳水化合物是还原糖，更优选碳水化合物是乳糖。 变性酶可以从生物体，优选微生物，更优选来自真菌，甚至更优选来自曲霉属的真菌，最优选来自黑曲霉获得。 碳水化合物结晶可以从纯的，部分纯化的或粗制的碳水化合物溶液中进行。 通常，碳水化合物首先被浓缩成过饱和溶液，之后温度降低，并且用碳水化合物晶体任选的接种引发结晶。 Mutarotase优选在结晶过程之前或期间加入。