公开(公告)号：WO2010141731A1

公开(公告)日：2010-12-09

申请号：PCT/US2010/037273

申请日：2010-06-03

Applicant: AAT BIOQUEST, INC. ,  DIWU, Zhenjun ,  LIAO, Jinfang

Inventor： DIWU, Zhenjun ,  LIAO, Jinfang

IPC: C09B44/10

CPC classification number: C09B29/0085 ,  A61K49/0017

Abstract: Azo heterocyclic compounds that are substantially non-fluorescent or only weakly fluorescent useful as energy acceptors. The chemically reactive compounds possess utility for labeling a wide variety of substances, including biomolecules with the resulted conjugates being highly useful for a variety of energy-transfer assays and applications. The biological conjugates of the disclosure exhibit little or no observable fluorescence and their fluorescence is significantly increased upon a biological stimulation.

Abstract translation: 基本上是非荧光的或仅用作能量受体的弱荧光的偶氮杂环化合物。 化学反应性化合物具有用于标记多种物质（包括生物分子）的效用，所得到的缀合物对于各种能量转移测定和应用是非常有用的。 本公开的生物共轭物显示很少或不可观察到的荧光，并且在生物刺激下其荧光显着增加。

公开(公告)号：WO2012012595A2

公开(公告)日：2012-01-26

申请号：PCT/US2011/044776

申请日：2011-07-21

Applicant: BECTON, DICKINSON AND COMPANY ,  AAT BIOQUEST, INC. ,  DIWU, Zhenjun ,  MENG, Qinglin ,  LIAO, Jinfang ,  GUO, Haitao ,  DUBROVSKY, Timothy ,  ABRAMS, Barnaby

Inventor： DIWU, Zhenjun ,  MENG, Qinglin ,  LIAO, Jinfang ,  GUO, Haitao ,  DUBROVSKY, Timothy ,  ABRAMS, Barnaby

IPC: C09B11/24 ,  C09B23/00 ,  C09B49/06 ,  G01N33/533

CPC classification number: C09B23/083 ,  C09B11/08 ,  C09B11/24 ,  C09B11/26 ,  C09B57/00 ,  C09B57/10 ,  G01N33/533

Abstract: Chemically reactive dyes that are intramolecularly crosslinked with a water-soluble bridge, their bioconjugates and their uses are described. Reactive fluorescent dyes that have a water-soluble bridge are superior to those of conjugates of spectrally non-crosslinked dyes or the dyes that are crosslinked with a hydrophobic bridge. The invention includes reactive fluorescent dyes, their biological conjugates and uses.

Abstract translation: 描述了与水溶性桥分子内交联的化学活性染料，它们的生物共轭物及其用途。 具有水溶性桥的反应性荧光染料优于光谱非交联染料的共轭物或与疏水桥交联的染料的那些。 本发明包括反应性荧光染料，它们的生物缀合物和用途。

公开(公告)号：WO2003099780A3

公开(公告)日：2003-12-04

申请号：PCT/US2003/016530

申请日：2003-05-23

Applicant: MOLECULAR DEVICES CORPORATION ,  DIWU, Zhenjun ,  LIAO, Jinfang ,  WANG, Zhiqiang

Inventor： DIWU, Zhenjun ,  LIAO, Jinfang ,  WANG, Zhiqiang

IPC: C12Q1/37

Abstract: Systems, including compositions, kits, and methods, suitable for performing enzyme assays, such as protease assays. The composition may include derivatives of rhodamine 110 that are substitued at the xanthylium nitrogens by a peptide moiety and a morpholine-4-carbonyl moiety, respectively. These compositions may be luminogenic substrates for a large variety of protease enzymes, with utility in a variety of sensitive protease enzyme assays.

公开(公告)号：WO2003099780A2

公开(公告)日：2003-12-04

申请号：PCT/US2003/016530

申请日：2003-05-23

Applicant: MOLECULAR DEVICES CORPORATION ,  DIWU, Zhenjun ,  LIAO, Jinfang ,  WANG, Zhiqiang

Inventor： DIWU, Zhenjun ,  LIAO, Jinfang ,  WANG, Zhiqiang

IPC: C07D

CPC classification number: C07K5/021 ,  C07D311/82 ,  C07D493/10 ,  C07K7/02 ,  C12Q1/37

Abstract: Systems, including compositions, kits, and methods, suitable for performing enzyme assays, such as protease assays. The composition may include derivatives of rhodamine 110 that are substitued at the xanthylium nitrogens by a peptide moiety and a morpholine-4-carbonyl moiety, respectively. These compositions may be luminogenic substrates for a large variety of protease enzymes, with utility in a variety of sensitive protease enzyme assays.

Abstract translation: 适用于进行酶测定的系统，包括组合物，试剂盒和方法，例如蛋白酶测定。 组合物可以包括分别由肽部分和吗啉-4-羰基部分在xanthylium氮取代的罗丹明110的衍生物。 这些组合物可以是用于各种蛋白酶的发光底物，可用于各种灵敏的蛋白酶测定。