公开(公告)号：WO2004036196A1

公开(公告)日：2004-04-29

申请号：PCT/US2003/032081

申请日：2003-10-08

Applicant: APPLERA CORPORATION

Inventor： SAGATELYAN, Dmitry, M. ,  SLETTNES, Tor

IPC: G01N21/64

CPC classification number: G01N21/274 ,  G01J1/42 ,  G01N21/645 ,  G01N2201/1241 ,  H03F3/08 ,  H04N5/235 ,  H04N5/2353 ,  H04N5/2355 ,  H04N5/243 ,  H04N5/35581

Abstract: A photo-detector 122 generated signal 125 is measured as a sample set 192 comprising a long signal and a short signal. The short signal is scaled to the value of the long signal if the long signal exceeds a dynamic range 131 associated with the photo detector 122. In one embodiment, the short signal is obtained during a short time interval that is at the approximate middle of a long time interval such that the short and long intervals share a common median time value 194. Given such symmetry, an approximately linear signal 190 yields a proportionality parameter between the long and short signals thereby allowing the short signal to be scaled. The proportionality parameter facilitates determination of an integration independent component of the photo detector signal that should be removed from the measured long and short signals before scaling. A plurality of sample sets 260 can also be processed such that each sample set overlaps with its neighboring sample set, thereby increasing the effective number of sample sets.

Abstract translation: 光检测器122产生的信号125被测量为包括长信号和短信号的采样组192。 如果长信号超过与光电检测器122相关联的动态范围131，则短信号被缩放到长信号的值。在一个实施例中，在短时间间隔内获得短信号，该短信号在大约中间 长时间间隔使得短和长间隔共享公共中值时间值194.给定这样的对称性，近似线性信号190在长信号和短信号之间产生比例参数，从而允许缩短短信号。 比例参数有助于确定光电探测器信号的积分独立分量，该分量在缩放之前应从测量的长信号和短信号中去除。 还可以处理多个样本集合260，使得每个样本集合与其相邻样本集重叠，从而增加样本集合的有效数量。