公开(公告)号：WO2015027112A1

公开(公告)日：2015-02-26

申请号：PCT/US2014/052186

申请日：2014-08-21

Applicant: APTON BIOSYSTEMS, INC.

Inventor： STAKER, Bryan, P. ,  LIU, Niandong ,  STAKER, Bart, Lee ,  MCLAUGHLIN, Michael, David

IPC: C40B20/04

CPC classification number: G01N33/58 ,  C12Q1/6804 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B20/04 ,  G01N27/4145 ,  G01N2458/10 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2525/197 ,  C12Q2525/204 ,  C12Q2565/607

Abstract: Electrical detection methods are used to identify and further characterize single-molecule target analytes such as proteins and nucleic acids. A composition including a probe region and a tail region is contacted with a target analyte. The probe region specifically binds to the target analyte. The tail region is coupled to the probe region, and includes a nucleic acid template for polynucleotide synthesis. When conditions are such that polynucleotide synthesis occurs along the tail region, one hydrogen ion is released for every nucleotide that is incorporated into the tail region. A transistor such as an ISFET detects and measures changes in ion concentration, and these measurements can be used to identify the tail region and thus characterize the corresponding target analyte.

Abstract translation: 电检测方法用于鉴定和进一步表征单分子靶分析物，如蛋白质和核酸。 包含探针区域和尾区域的组合物与目标分析物接触。 探针区域特异性结合目标分析物。 尾区域耦合到探针区域，并且包括用于多核苷酸合成的核酸模板。 当条件使得沿着尾部区域发生多核苷酸合成时，对于结合到尾部区域的每个核苷酸释放一个氢离子。 诸如ISFET的晶体管检测并测量离子浓度的变化，并且可以使用这些测量来识别尾部区域，从而表征相应的目标分析物。