公开(公告)号：WO2019126209A1

公开(公告)日：2019-06-27

申请号：PCT/US2018/066291

申请日：2018-12-18

Applicant: CELLULAR RESEARCH, INC.

Inventor： LAM, Gretchen, Yinbon ,  SHUM, Eleen

IPC: C12Q1/6869 ,  C12Q1/6804

CPC classification number: C12Q1/6869 ,  C12Q1/6804 ,  C12Q2525/179 ,  C12Q2563/131 ,  C12Q2563/179

Abstract: Disclosed herein are methods, compositions, and kits for barcoding nucleic acids. For example, particles for barcoding are provided, where the particle comprises: a first plurality of oligonucleotide barcodes each comprising a target-binding region, and a second plurality of oligonucleotides each comprising a target-specific target-binding region.

公开(公告)号：WO2019023776A1

公开(公告)日：2019-02-07

申请号：PCT/BR2018/050261

申请日：2018-07-27

Applicant: NATURA COSMÉTICOS S.A.

Inventor： RODRIGUES DE PAULA, Leonardo

IPC: G01N33/53 ,  A61K8/00 ,  A61Q19/00 ,  G01N21/64 ,  C12Q1/6804

CPC classification number: A61K8/00 ,  A61Q19/00 ,  C12Q1/6804 ,  G01N21/64 ,  G01N33/53

Abstract: A presente invenção trata de um método para caracterização de hidratação bioativa (ou ativa ou dinâmica ou biológica)a partir do emprego de técnicas para avaliação de expressão gênica e proteica que permite identificar novos ingredientes ou combinação de ingredientes eficientes no estímulo dos cinco mecanismos endógenos da pele associados à dita hidratação.

公开(公告)号：WO2018076018A1

公开(公告)日：2018-04-26

申请号：PCT/US2017/057916

申请日：2017-10-23

Applicant: EXOSOME DIAGNOSTICS, INC. ,  SKOG, Johan ,  CHAKRABORTTY, Sudipto ,  CHAN, Dalin ,  VALENTINO, Michael ,  TADIGOTLA, Vasisht ,  KITCHEN, Robert ,  GRIMM, Dominik ,  YU, Wei

Inventor： SKOG, Johan ,  CHAKRABORTTY, Sudipto ,  CHAN, Dalin ,  VALENTINO, Michael ,  TADIGOTLA, Vasisht ,  KITCHEN, Robert ,  GRIMM, Dominik ,  YU, Wei

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6804 ,  C12Q1/6869 ,  C12Q2521/107 ,  C12Q2525/191 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/161

Abstract: The invention provides a series of steps that prepare nucleic acids (RNA and/or DNA) isolated from extracellular vesicles for sequencing. This enables a wide diversity of RNAs and/or DNAs, to be efficiently detected. These can then be used to identify various attributes such as gene expression, alternative splicing, and the detection of both somatic and germline mutations including single nucleotide variants (SNV) and structural variations (insertions/deletions, fusions, inversions).

Abstract translation: 本发明提供了一系列制备从细胞外囊泡分离的核酸（RNA和/或DNA）用于测序的步骤。 这使得RNA和/或DNA的多样性得以有效检测。 然后这些可用于鉴定各种属性，如基因表达，选择性剪接，体细胞突变和种系突变（包括单核苷酸变体（SNV）和结构变异（插入/缺失，融合，倒位）的检测）。

公开(公告)号：WO2017153378A1

公开(公告)日：2017-09-14

申请号：PCT/EP2017/055269

申请日：2017-03-07

Applicant: UNIVERSITÉ DE TECHNOLOGIE DE TROYES

Inventor： BRUYANT, Aurélien ,  VAILLANT, Julien ,  WU, Tzu-Heng ,  LIN, Chii-Wann

IPC: G01B9/02 ,  G01N21/552 ,  G01N21/45 ,  G01D5/26 ,  G01N33/68

CPC classification number: G01B9/02051 ,  C12Q1/6804 ,  C12Q1/6816 ,  G01B9/02004 ,  G01N21/45 ,  G01N21/553 ,  G01N21/554 ,  G01N33/68 ,  C12Q2525/205 ,  C12Q2565/601

Abstract: The invention relates to a compact and portable interferometer(4)comprising an optical source (40) generating a first beam (B); an optical chip comprising a first reflective layer (42) and a second reflective layer (45) separated by a dielectric spacer layer (41), the first reflective layer splitting the first beam into a reference beam (BR) and a second beam (B2), the second reflective layer reflecting the second beam into a signal beam (BS) overlapping the reference beam to create an interference signal(I); a controlled phase modulation means; and a photo-detector(43)receiving the interference signal. The invention also relates to abio-chemical sensor comprising an interferometer (4) wherein the second reflective layer is an optically-resonant layer chemically functionalized. The invention eventually relates to a measurement device comprising said interferometer or said bio-chemical sensor and a signal analyzing unit processing information from the photo-detector.

Abstract translation: 本发明涉及一种紧凑且便携式的干涉仪（4），其包括产生第一光束（B）的光源（40）; 包括由电介质隔离层（41）分隔的第一反射层（42）和第二反射层（45）的光学芯片，所述第一反射层将所述第一光束分成参考光束（BR）和第二光束（B2 ），所述第二反射层将所述第二光束反射为与所述参考光束重叠的信号光束（BS）以产生干涉信号（I）; 受控相位调制装置; 和接收干涉信号的光电检测器（43）。 本发明还涉及包括干涉仪（4）的非化学传感器，其中第二反射层是化学官能化的光学谐振层。 本发明最终涉及一种包括所述干涉仪或所述生物化学传感器的测量装置和一个处理来自光电检测器的信息的信号分析单元。

公开(公告)号：WO2017100283A1

公开(公告)日：2017-06-15

申请号：PCT/US2016/065325

申请日：2016-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： HEIL, Korbinian ,  NOTKA, Frank ,  GEE, Kyle ,  DALLWIG, Jason ,  KUHN, Phillip ,  CORRY, Schuyler

IPC: C12Q1/68

CPC classification number: C12Q1/6804 ,  C12Q1/6834 ,  C12Q2563/107 ,  C12Q2563/149 ,  C12Q2565/537

Abstract: This disclosure relates to methods, compositions, and related apparatuses for detection and quantification of nucleic acid molecules associated with a solid surface. Methods can include detecting or quantifying at least one nucleic acid molecule associated with a surface by contacting the at least one nucleic acid molecule with a fluorophore, wherein the fluorophore emits increased fluorescence at a wavelength when in contact with nucleic acid molecule, and detecting or measuring fluorescence from the fluorophore at the wavelength. Compositions can comprise a fluorophore and a nucleic acid molecule, wherein the nucleic acid molecule is associated with a surface, and the fluorophore has the property of emitting increased fluorescence at a wavelength when in contact with a nucleic acid molecule.

Abstract translation: 本公开涉及用于检测和定量与固体表面相关的核酸分子的方法，组合物和相关装置。 方法可以包括通过使至少一种核酸分子与荧光团接触来检测或定量至少一种与表面相关的核酸分子，其中荧光团在与核酸分子接触时在波长处发射增加的荧光，并且检测或测量 荧光团在波长处的荧光。 组合物可以包含荧光团和核酸分子，其中核酸分子与表面结合，并且荧光团在与核酸分子接触时具有在波长处发射增加的荧光的性质。

公开(公告)号：WO2017100251A1

公开(公告)日：2017-06-15

申请号：PCT/US2016/065270

申请日：2016-12-07

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： AGASTI, Sarit ,  YIN, Peng ,  YAGHI, Omar ,  GOPALKRISHNAN, Nikhil

IPC: G01N33/533 ,  G01N33/58 ,  C12N15/115 ,  C12Q1/68

CPC classification number: G01N33/58 ,  C12Q1/6804 ,  C12Q1/6837 ,  G01N2458/10 ,  C12Q2525/161 ,  C12Q2563/179 ,  C12Q2565/514 ,  C12Q2537/125

Abstract: Embodiments of the present disclosure relate molecular (e.g., proteomic and genomic) profiling of one or more cells using quantitative-based nucleic acid detection methods.

Abstract translation: 本公开的实施方案涉及使用基于定量的核酸检测方法的一种或多种细胞的分子（例如，蛋白质组和基因组）分析。

公开(公告)号：WO2016196691A2

公开(公告)日：2016-12-08

申请号：PCT/US2016/035357

申请日：2016-06-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： HEATH, James R. ,  PENG, Songming

IPC: G01N33/569 ,  C12Q1/68

CPC classification number: G01N33/56972 ,  C12Q1/6804 ,  G01N15/14 ,  G01N33/574 ,  G01N33/6878 ,  G01N2015/008 ,  G01N2015/149 ,  G01N2333/70539 ,  G01N2458/10 ,  C12Q2563/131 ,  C12Q2563/143 ,  C12Q2563/155 ,  C12Q2565/514

Abstract: Compositions and methods for isolating patient -derived antigen-specific T cells include an antigen complex having a polynucleotide barcoded nanoparticle sorting agent complexed with a peptide-loaded streptavidin major histocompatability complex (MHC) tetramer, the barcoding technology allowing for high fidelity screening of a library of the antigen complexes to readily isolate and identify antigen-specific T cells.

Abstract translation: 用于分离患者衍生的抗原特异性T细胞的组合物和方法包括具有与加载肽的链霉亲和素主要组织相容性复合物（MHC）四聚体复合的多核苷酸条形码纳米颗粒分选试剂的抗原复合物，所述条形码技术允许文库的高保真度筛选 的抗原复合物以容易地分离和鉴定抗原特异性T细胞。

公开(公告)号：WO2016187234A1

公开(公告)日：2016-11-24

申请号：PCT/US2016/032936

申请日：2016-05-17

Applicant: KARIUS, INC.

Inventor： BLAUWKAMP, Timothy, A. ,  CHRISTIANS, Fred ,  VILFAN, Igor, D. ,  SMITH, Scott ,  KERTESZ, Michael

IPC: C12Q1/68 ,  G06F19/22 ,  C40B40/06 ,  G01N33/483

CPC classification number: C12Q1/6806 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6888 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2537/159

Abstract: This disclosure provides methods and compositions that are useful for enriching a particular population of nucleic acids (a "population of interest") within a complex mixture of nucleic acids. The population of interest may make up a minor portion of a complex mixture of nucleic acids. The methods and compositions provided herein are useful for detecting, predicting, diagnosing, or monitoring a disease or disorder, particularly a disease or disorder caused by a foreign microbe or pathogen.

Abstract translation: 本公开提供了可用于在核酸的复杂混合物中富集特定的核酸群体（“感兴趣群体”）的方法和组合物。 感兴趣的群体可能构成复杂的核酸混合物的一小部分。 本文提供的方法和组合物可用于检测，预测，诊断或监测疾病或病症，特别是由外来微生物或病原体引起的疾病或病症。

公开(公告)号：WO2016164419A1

公开(公告)日：2016-10-13

申请号：PCT/US2016/026157

申请日：2016-04-06

Applicant: CENTRILLION TECHNOLOGY HOLDINGS CORPORATION ,  ZHOU, Wei

Inventor： ZHOU, Wei ,  EDWARDS, Jeremy ,  COSTA, Justin

IPC: A61K33/24 ,  C12Q1/68

CPC classification number: C07K16/44 ,  C07K16/46 ,  C07K2317/92 ,  C07K2319/01 ,  C07K2319/20 ,  C12N15/1006 ,  C12N15/1065 ,  C12N15/1093 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6811 ,  C12Q2523/303 ,  C12Q2535/122 ,  C12Q2537/164 ,  C12Q2565/501 ,  C12Q2600/156

Abstract: Provided herein are methods and compositions for analyzing epigenetic modifications of genomes. The methods and compositions are suited for complete epigenome sequencing of any modification for which an antibody or an affinity binding agent has been developed. In one aspect, provided herein is a method for analyzing epigenetic modification of a genome. In some embodiments of aspects provided herein, the method further comprises sequencing a sequencing library to generate sequence reads, and assembling the sequence reads with aid of a positional barcode sequence information. In some embodiments of aspects provided herein, the method further comprises determining a location of at least two different epigenetic modifications of the nucleic acid. Another aspect of the present disclosure provides a kit for analyzing an epigenetic modification of a nucleic acid.

Abstract translation: 本文提供用于分析基因组的表观遗传修饰的方法和组合物。 所述方法和组合物适用于已经开发了抗体或亲和力结合剂的任何修饰的完整表观基因组测序。 一方面，本文提供了一种用于分析基因组的表观遗传修饰的方法。 在本文提供的方面的一些实施方案中，该方法还包括测序序列库以产生序列读取，以及借助位置条形码序列信息组装序列读取。 在本文提供的方面的一些实施方案中，所述方法还包括确定核酸的至少两种不同表观遗传修饰的位置。 本公开的另一方面提供了用于分析核酸的表观遗传修饰的试剂盒。

公开(公告)号：WO2016149253A1

公开(公告)日：2016-09-22

申请号：PCT/US2016/022441

申请日：2016-03-15

Applicant: DOTS TECHNOLOGY CORP.

Inventor： GILBOA-GEFFEN, Adi ,  BROWN, Renuka Babu ,  KEPLER, John H. ,  ROHATGI, Gaurav ,  WALKER, Philip Charles ,  BATES, Mark ,  PAGE, Christopher James

IPC: B01L3/00 ,  C12Q1/68 ,  G01N30/00 ,  G01N33/02

CPC classification number: B01L3/502 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0605 ,  B01L2200/0689 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/0609 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0694 ,  C12Q1/6804 ,  G01N1/08 ,  G01N1/286 ,  G01N33/02 ,  G01N33/5308 ,  G01N2001/2866 ,  C12Q2525/205 ,  C12Q2563/107 ,  C12Q2565/1015 ,  C12Q2565/107

Abstract: The present invention provides systems, devices and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The detection system includes a separate sample pickup, one or more disposables for receiving and processing a test sample and a detection device that can execute an allergen detection testing in minutes. The present detection system and device is compact and portable.

Abstract translation: 本发明提供用于检测样品中特定食品样品中一种或多种过敏原的存在和/或不存在的系统，装置和方法。 检测系统包括单独的样品拾取器，用于接收和处理测试样品的一个或多个一次性物品以及可以在几分钟内执行变应原检测测试的检测装置。 本检测系统和设备紧凑便携。