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    BOTTLENECK SEQUENCING
    1.
    发明申请
    BOTTLENECK SEQUENCING 审中-公开
    BOTTLENECK测序

    公开(公告)号：WO2017132438A1

    公开(公告)日：2017-08-03

    申请号：PCT/US2017/015229

    申请日：2017-01-27

    Applicant: THE JOHNS HOPKINS UNIVERSITY

    Inventor： VOGELSTEIN, Bert ,  KINZLER, Kenneth ,  HOANG, Margaret ,  PAPADOPOULOS, Nickolas

    IPC: C12Q1/68 ,  C40B20/04

    CPC classification number: C12Q1/6869 ,  C12Q1/6844 ,  C40B20/04 ,  C12Q2525/151 ,  C12Q2525/191 ,  C12Q2565/514

    Abstract: Bottleneck Sequencing System (BotSeqS) is a next-generation sequencing method that simultaneously quantifies rare somatic point mutations across the mitochondrial and nuclear genomes. BotSeqS combines molecular barcoding with a simple dilution step immediately prior to library amplification. BotSeqS can be used to show age and tissue-dependent accumulations of rare mutations and demonstrate that somatic mutational burden in normal tissues can vary by several orders of magnitude, depending on biologic and environmental factors. BotSeqS has been used to show major differences between the mutational patterns of the mitochondrial and nuclear genomes in normal tissues. Lastly, BotSeqS has shown that the mutation spectra of normal tissues were different from each other, but similar to those of the cancers that arose in them.

    Abstract translation: 瓶颈测序系统（BotSeqS）是一种新一代测序方法，可同时量化线粒体和核基因组中的罕见体细胞点突变。 BotSeqS在文库扩增之前将分子条形码与简单稀释步骤结合在一起。 BotSeqS可以用于显示年龄和组织依赖性罕见突变的累积，并且证明正常组织中的体细胞突变负荷可以根据生物和环境因素而变化几个数量级。 BotSeqS已被用于显示正常组织中线粒体和核基因组的突变模式之间的主要差异。 最后，BotSeqS已经表明，正常组织的突变谱彼此不同，但与它们中出现的癌症的突变谱相似。

    DIGITAL ANALYSIS OF MOLECULAR ANALYTES USING ELECTRICAL METHODS
    3.
    发明申请
    DIGITAL ANALYSIS OF MOLECULAR ANALYTES USING ELECTRICAL METHODS 审中-公开
    使用电气方法的分子分析的数字分析

    公开(公告)号：WO2015027112A1

    公开(公告)日：2015-02-26

    申请号：PCT/US2014/052186

    申请日：2014-08-21

    Applicant: APTON BIOSYSTEMS, INC.

    Inventor： STAKER, Bryan, P. ,  LIU, Niandong ,  STAKER, Bart, Lee ,  MCLAUGHLIN, Michael, David

    IPC: C40B20/04

    CPC classification number: G01N33/58 ,  C12Q1/6804 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B20/04 ,  G01N27/4145 ,  G01N2458/10 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2525/197 ,  C12Q2525/204 ,  C12Q2565/607

    Abstract: Electrical detection methods are used to identify and further characterize single-molecule target analytes such as proteins and nucleic acids. A composition including a probe region and a tail region is contacted with a target analyte. The probe region specifically binds to the target analyte. The tail region is coupled to the probe region, and includes a nucleic acid template for polynucleotide synthesis. When conditions are such that polynucleotide synthesis occurs along the tail region, one hydrogen ion is released for every nucleotide that is incorporated into the tail region. A transistor such as an ISFET detects and measures changes in ion concentration, and these measurements can be used to identify the tail region and thus characterize the corresponding target analyte.

    Abstract translation: 电检测方法用于鉴定和进一步表征单分子靶分析物，如蛋白质和核酸。 包含探针区域和尾区域的组合物与目标分析物接触。 探针区域特异性结合目标分析物。 尾区域耦合到探针区域，并且包括用于多核苷酸合成的核酸模板。 当条件使得沿着尾部区域发生多核苷酸合成时，对于结合到尾部区域的每个核苷酸释放一个氢离子。 诸如ISFET的晶体管检测并测量离子浓度的变化，并且可以使用这些测量来识别尾部区域，从而表征相应的目标分析物。

    MOLECULAR CODE SYSTEMS
    4.
    发明申请
    MOLECULAR CODE SYSTEMS 审中-公开
    分子代码系统

    公开(公告)号：WO2013143014A1

    公开(公告)日：2013-10-03

    申请号：PCT/CH2013/000052

    申请日：2013-03-26

    Applicant: ETH ZURICH

    Inventor： GRASS, Robert N. ,  STARK, Wendelin Jan

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  B01J2219/00572 ,  B01L3/54 ,  C12Q1/68 ,  C40B20/04 ,  C40B50/16 ,  C12Q2527/125 ,  C12Q2563/149 ,  C12Q2563/185

    Abstract: The present invention relates to a method for protecting and. recovering nucleic acids. This method may be applied in a verification method of products using molecular code systems or it may be applied in a storage method for nucleic acids. The invention further provides specific particles, their use in secure marking; products suitable for such verification method, to processes for manufacturing such products and methods for reading the information.

    Abstract translation: 本发明涉及一种保护和 回收核酸。 该方法可以应用于使用分子代码系统的产品的验证方法，或者可以应用于核酸的储存方法中。 本发明还提供特定的颗粒，它们在安全标记中的用途; 适用于这种验证方法的产品，用于制造这些产品的方法和用于阅读信息的方法。

    TWO-PRIMER SEQUENCING FOR HIGH-THROUGHPUT EXPRESSION ANALYSIS
    6.
    发明申请
    TWO-PRIMER SEQUENCING FOR HIGH-THROUGHPUT EXPRESSION ANALYSIS 审中-公开
    用于高通量表达分析的两个引导序列

    公开(公告)号：WO2009082750A1

    公开(公告)日：2009-07-02

    申请号：PCT/US2008/088139

    申请日：2008-12-23

    Applicant: HELICOS BIOSCIENCES CORPORATION ,  NICKERSON, Elizabeth ,  CAUSEY, Marie Sutherlin

    Inventor： NICKERSON, Elizabeth ,  CAUSEY, Marie Sutherlin

    IPC: C12Q1/68

    CPC classification number: C40B70/00 ,  B01J2219/0054 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  C12Q1/6869 ,  C40B20/04 ,  C40B80/00 ,  C12Q2525/197 ,  C12Q2525/161 ,  C12Q2525/155

    Abstract: The disclosure provides a method of sequencing a nucleic acid molecule that contains two or more target regions to be sequenced (such as, for example, barcodes). The invention is advantageous for sequencing by synthesis two or more target regions whose combined lengths plus the length of any intermediate sequence exceeds the available read length on a given sequencing platform. The methods of the invention utilize nucleic acid constructs containing at least the following elements: a complement of a first universal primer, a first target sequence, an optional polynucleotide spacer, a complement of a second universal primer, and a second target sequence. A first round of sequencing by synthesis is performed to sequence the first target sequence by elongating the first universal primer. Once the sequence of the first target region is obtained, and before the complement of the second primer is reached, the first round of sequencing is terminated. Thereafter, a second round of sequencing by synthesis is initiated--this time, by elongating the second universal primer, thereby sequencing the second target region.

    Abstract translation: 本公开提供了对含有待测序的两个或多个靶区域（例如条形码）的核酸分子进行测序的方法。 本发明有利于通过合成对组合长度加上任何中间序列的长度超过给定测序平台上的可用读取长度的两个或多个靶区进行排序。 本发明的方法利用至少含有以下元件的核酸构建体：第一通用引物的补体，第一靶序列，任选的多核苷酸间隔物，第二通用引物的补体和第二靶序列。 进行通过合成的第一轮测序以通过延长第一通用引物来对第一靶序列进行序列化。 一旦获得了第一个靶区的序列，并且在第二个引物的补体之前，第一轮测序终止。 此后，开始第二轮合成测序 - 这次通过延长第二通用引物，从而对第二目标区进行测序。

    SYSTEMS AND METHODS FOR ANALYZING NANOREPORTERS
    8.
    发明申请
    SYSTEMS AND METHODS FOR ANALYZING NANOREPORTERS 审中-公开
    用于分析纳米载体的系统和方法

    公开(公告)号：WO2007139766A2

    公开(公告)日：2007-12-06

    申请号：PCT/US2007/012130

    申请日：2007-05-21

    Applicant: NANOSTRING TECHNOLOGIES, INC. ,  HWANG, Jenq-Neng ,  MITTON, Jeffrey, D.

    Inventor： HWANG, Jenq-Neng ,  MITTON, Jeffrey, D.

    IPC: G06F19/00

    CPC classification number: B82Y30/00 ,  C40B20/04 ,  C40B70/00 ,  G06F19/16 ,  G06F19/20

    Abstract: Methods, computers, and computer program products for detecting the presence of a probe within a sample overlayed on a substrate are provided. The probe comprises a plurality of spatially arranged labels. A data storage module stores a plurality of light images, where each light image has light from the sample at a corresponding wavelength range in a plurality of different wavelength ranges. A label identification module identifies a plurality of labels in the plurality of light images that are proximate to each other on the substrate. A spatial order of the plurality of labels determines a string sequence of the plurality of labels. A probe identification module determines whether the string sequence of the plurality of labels comprises a valid reporter sequence.

    Abstract translation: 提供了用于检测覆盖在基底上的样品中探针存在的方法，计算机和计算机程序产品。 探针包括多个空间布置的标签。 数据存储模块存储多个光图像，其中每个光图像具有来自多个不同波长范围内相应波长范围的样本的光。 标签识别模块识别在基板上彼此靠近的多个光图像中的多个标签。 多个标签的空间顺序确定多个标签的字符串序列。 探针识别模块确定多个标签的字符串序列是否包含有效的报告序列。

    CREATION OF FUNCTIONALIZED MICROPARTICLE LIBRARIES BY OLIGONUCLEOTIDE LIGATION OR ELONGATION
    9.
    发明申请
    CREATION OF FUNCTIONALIZED MICROPARTICLE LIBRARIES BY OLIGONUCLEOTIDE LIGATION OR ELONGATION 审中-公开
    通过寡核苷酸结合或延伸产生功能化的微生物图谱

    公开(公告)号：WO2006130347A2

    公开(公告)日：2006-12-07

    申请号：PCT/US2006/019203

    申请日：2006-05-17

    Applicant: BIOARRAY SOLUTIONS, LTD. ,  SEUL, Michael ,  YANG, Jiacheng ,  BANERJEE, Sukanta

    Inventor： SEUL, Michael ,  YANG, Jiacheng ,  BANERJEE, Sukanta

    CPC classification number: B01J19/0046 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12N15/1093 ,  C40B20/04 ,  C40B50/14

    Abstract: Disclosed are methods of for constructing a bead-displayed library of oligonucleotide probes (or sequence-modified capture moieties such as protein-nucleic acid conjugates) by ligation of a capture probe, having an analyte-specific sequence, to an anchor probe that is attached, at its 5 '-end, (or possibly at the 3' end) to an encoded carrier such as a color-coded microparticle ("bead"). Such a library can also be constructed by elongation of an anchor probe, using a second probe as the elongation template, wherein the second probe has an anchor-specific subsequence and an analyte-specific subsequence.

    Abstract translation: 公开了通过将具有分析物特异性序列的捕获探针连接到所连接的锚定探针上来构建寡核苷酸探针（或序列修饰的捕获部分，例如蛋白质 - 核酸缀合物）的珠显示文库的方法 在5'端，（或可能在3'端）与经编码的载体（如着色微粒（“珠”））相连。 也可以使用第二探针作为延伸模板，通过锚定探针的伸长来构建这种文库，其中第二探针具有锚特异性亚序列和分析物特异性亚序列。

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